Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells
Curcuma longa (tumeric) is listed in several therapeutic regimens used in the management of cancer. The present study investigated the potential cytotoxic effects of ethanol extract of Curcuma longa (turmeric) leaves on HepG2 (a human hepatocellular carcinoma cell line), and MCF-7 (a human mammary...
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| Format: | Article |
| Language: | English |
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Fountain University Osogbo
2022-12-01
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| Series: | Fountain Journal of Natural and Applied Sciences (FUJNAS) |
| Subjects: | |
| Online Access: | https://fountainjournals.com/index.php/FUJNAS/article/view/411 |
| _version_ | 1797665731831136256 |
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| author | Ridwan Lawal A B. James Rafiu Shaibu O. P. Okoye S. A. Odetunde A. C. Ajibare |
| author_facet | Ridwan Lawal A B. James Rafiu Shaibu O. P. Okoye S. A. Odetunde A. C. Ajibare |
| author_sort | Ridwan Lawal |
| collection | DOAJ |
| description |
Curcuma longa (tumeric) is listed in several therapeutic regimens used in the management of cancer. The present study investigated the potential cytotoxic effects of ethanol extract of Curcuma longa (turmeric) leaves on HepG2 (a human hepatocellular carcinoma cell line), and MCF-7 (a human mammary gland adenocarcinoma cell line) cells. The HepG2 and MCF-7 cells were exposed to different concentrations; 2, 0.2, 0.02, 0.002, 0.0002, 0.00002, 0.000002 μg/ml, of the ethanol extract of C. longa leaves. The Water-Soluble tetrazolium salt (WST-1) assay was used to estimate the cell viability of the cells after which the percentage proliferation was computed. The results of this study indicated that C. longa had cytotoxic effect on the different cell lines, caused a decrease in proliferation and it is more selective to breast cancer cell lines based on the results of WST-1 analysis on the HepG2 and MCF-7 cells. These results suggest that C. longa leaf possesses cytotoxic properties, which can be exploited to develop novel cytotoxic drugs for cancer management.
Keywords: Curcuma longa, turmeric, HepG2, MCF-7, WST-1, proliferation, cytotoxic, cancer
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| first_indexed | 2024-03-11T19:48:50Z |
| format | Article |
| id | doaj.art-c9d0febece7e4594babc23a1b6e50978 |
| institution | Directory Open Access Journal |
| issn | 2350-1863 2354-337X |
| language | English |
| last_indexed | 2024-03-11T19:48:50Z |
| publishDate | 2022-12-01 |
| publisher | Fountain University Osogbo |
| record_format | Article |
| series | Fountain Journal of Natural and Applied Sciences (FUJNAS) |
| spelling | doaj.art-c9d0febece7e4594babc23a1b6e509782023-10-05T16:54:50ZengFountain University OsogboFountain Journal of Natural and Applied Sciences (FUJNAS)2350-18632354-337X2022-12-01112Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cellsRidwan Lawal0A B. James1Rafiu Shaibu2O. P. Okoye3S. A. Odetunde4A. C. Ajibare5Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.Department of Biochemistry, Nigerian Institute of Medical Research, Yaba, Lagos, NigeriaDepartment of Chemistry, Faculty of Science, University of Lagos, Lagos, NigeriaDepartment of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria. Curcuma longa (tumeric) is listed in several therapeutic regimens used in the management of cancer. The present study investigated the potential cytotoxic effects of ethanol extract of Curcuma longa (turmeric) leaves on HepG2 (a human hepatocellular carcinoma cell line), and MCF-7 (a human mammary gland adenocarcinoma cell line) cells. The HepG2 and MCF-7 cells were exposed to different concentrations; 2, 0.2, 0.02, 0.002, 0.0002, 0.00002, 0.000002 μg/ml, of the ethanol extract of C. longa leaves. The Water-Soluble tetrazolium salt (WST-1) assay was used to estimate the cell viability of the cells after which the percentage proliferation was computed. The results of this study indicated that C. longa had cytotoxic effect on the different cell lines, caused a decrease in proliferation and it is more selective to breast cancer cell lines based on the results of WST-1 analysis on the HepG2 and MCF-7 cells. These results suggest that C. longa leaf possesses cytotoxic properties, which can be exploited to develop novel cytotoxic drugs for cancer management. Keywords: Curcuma longa, turmeric, HepG2, MCF-7, WST-1, proliferation, cytotoxic, cancer https://fountainjournals.com/index.php/FUJNAS/article/view/411Curcuma longacytotoxicHepG2MCF-7WST-1cancer |
| spellingShingle | Ridwan Lawal A B. James Rafiu Shaibu O. P. Okoye S. A. Odetunde A. C. Ajibare Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells Fountain Journal of Natural and Applied Sciences (FUJNAS) Curcuma longa cytotoxic HepG2 MCF-7 WST-1 cancer |
| title | Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells |
| title_full | Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells |
| title_fullStr | Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells |
| title_full_unstemmed | Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells |
| title_short | Cytotoxic effects of Curcuma longa leaves on MCF-7 and HepG2 cells |
| title_sort | cytotoxic effects of curcuma longa leaves on mcf 7 and hepg2 cells |
| topic | Curcuma longa cytotoxic HepG2 MCF-7 WST-1 cancer |
| url | https://fountainjournals.com/index.php/FUJNAS/article/view/411 |
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