Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function
Summary: The rapid transit from hypoxia to normoxia in the lung that follows the first breath in newborn mice coincides with alveolar macrophage (AM) differentiation. However, whether sensing of oxygen affects AM maturation and function has not been previously explored. We have generated mice whose...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-08-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718311240 |
| _version_ | 1819014765366738944 |
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| author | Helena M. Izquierdo Paola Brandi Manuel-José Gómez Ruth Conde-Garrosa Elena Priego Michel Enamorado Sarai Martínez-Cano Iria Sánchez Laura Conejero Daniel Jimenez-Carretero Silvia Martín-Puig Martin Guilliams David Sancho |
| author_facet | Helena M. Izquierdo Paola Brandi Manuel-José Gómez Ruth Conde-Garrosa Elena Priego Michel Enamorado Sarai Martínez-Cano Iria Sánchez Laura Conejero Daniel Jimenez-Carretero Silvia Martín-Puig Martin Guilliams David Sancho |
| author_sort | Helena M. Izquierdo |
| collection | DOAJ |
| description | Summary: The rapid transit from hypoxia to normoxia in the lung that follows the first breath in newborn mice coincides with alveolar macrophage (AM) differentiation. However, whether sensing of oxygen affects AM maturation and function has not been previously explored. We have generated mice whose AMs show a deficient ability to sense oxygen after birth by deleting Vhl, a negative regulator of HIF transcription factors, in the CD11c compartment (CD11cΔVhl mice). VHL-deficient AMs show an immature-like phenotype and an impaired self-renewal capacity in vivo that persists upon culture ex vivo. VHL-deficient phenotype is intrinsic in AMs derived from monocyte precursors in mixed bone marrow chimeras. Moreover, unlike control Vhlfl/fl, AMs from CD11cΔVhl mice do not reverse pulmonary alveolar proteinosis when transplanted into Csf2rb−/− mice, demonstrating that VHL contributes to AM-mediated surfactant clearance. Thus, our results suggest that optimal AM terminal differentiation, self-renewal, and homeostatic function requires their intact oxygen-sensing capacity. : Izquierdo et al. show that the absence of VHL in lung alveolar macrophages prevents their terminal maturation. VHL-deficient alveolar macrophages induce HIF-target gene expression and are partially desensitized to oxygen. Moreover, they have a decreased self-renewal and surfactant clearance capacity, suggesting that oxygen sensing contributes to alveolar macrophage functional maturation. Keywords: alveolar macrophage, oxygen, VHL, HIF, self-renewal, differentiation, surfactant, lung, glycolysis, lipid metabolism |
| first_indexed | 2024-12-21T02:21:02Z |
| format | Article |
| id | doaj.art-c9d11d4f274940618ebe34423d921468 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T02:21:02Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c9d11d4f274940618ebe34423d9214682022-12-21T19:19:09ZengElsevierCell Reports2211-12472018-08-0124717381746Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and FunctionHelena M. Izquierdo0Paola Brandi1Manuel-José Gómez2Ruth Conde-Garrosa3Elena Priego4Michel Enamorado5Sarai Martínez-Cano6Iria Sánchez7Laura Conejero8Daniel Jimenez-Carretero9Silvia Martín-Puig10Martin Guilliams11David Sancho12Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, SpainLaboratory of Myeloid Cell Ontogeny and Functional Specialisation, VIB-UGhent Centre for Inflammation Research, Ghent 9052, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent 9052, BelgiumCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, Spain; Corresponding authorSummary: The rapid transit from hypoxia to normoxia in the lung that follows the first breath in newborn mice coincides with alveolar macrophage (AM) differentiation. However, whether sensing of oxygen affects AM maturation and function has not been previously explored. We have generated mice whose AMs show a deficient ability to sense oxygen after birth by deleting Vhl, a negative regulator of HIF transcription factors, in the CD11c compartment (CD11cΔVhl mice). VHL-deficient AMs show an immature-like phenotype and an impaired self-renewal capacity in vivo that persists upon culture ex vivo. VHL-deficient phenotype is intrinsic in AMs derived from monocyte precursors in mixed bone marrow chimeras. Moreover, unlike control Vhlfl/fl, AMs from CD11cΔVhl mice do not reverse pulmonary alveolar proteinosis when transplanted into Csf2rb−/− mice, demonstrating that VHL contributes to AM-mediated surfactant clearance. Thus, our results suggest that optimal AM terminal differentiation, self-renewal, and homeostatic function requires their intact oxygen-sensing capacity. : Izquierdo et al. show that the absence of VHL in lung alveolar macrophages prevents their terminal maturation. VHL-deficient alveolar macrophages induce HIF-target gene expression and are partially desensitized to oxygen. Moreover, they have a decreased self-renewal and surfactant clearance capacity, suggesting that oxygen sensing contributes to alveolar macrophage functional maturation. Keywords: alveolar macrophage, oxygen, VHL, HIF, self-renewal, differentiation, surfactant, lung, glycolysis, lipid metabolismhttp://www.sciencedirect.com/science/article/pii/S2211124718311240 |
| spellingShingle | Helena M. Izquierdo Paola Brandi Manuel-José Gómez Ruth Conde-Garrosa Elena Priego Michel Enamorado Sarai Martínez-Cano Iria Sánchez Laura Conejero Daniel Jimenez-Carretero Silvia Martín-Puig Martin Guilliams David Sancho Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function Cell Reports |
| title | Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function |
| title_full | Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function |
| title_fullStr | Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function |
| title_full_unstemmed | Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function |
| title_short | Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function |
| title_sort | von hippel lindau protein is required for optimal alveolar macrophage terminal differentiation self renewal and function |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718311240 |
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