Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene
MicroRNA-155 (miR155) is overexpressed in various inflammatory diseases and cancer, in which bone resorption and osteolysis are frequently observed. However, the role of miR155 on osteogenesis and bone mass phenotype is still unknown. Here, we report a low bone mass phenotype in the long bone of Mir...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/77742 |
| _version_ | 1828064778092806144 |
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| author | Zhichao Zheng Lihong Wu Zhicong Li Ruoshu Tang Hongtao Li Yinyin Huang Tianqi Wang Shaofen Xu Haoyu Cheng Zhitong Ye Dong Xiao Xiaolin Lin Gang Wu Richard T Jaspers Janak L Pathak |
| author_facet | Zhichao Zheng Lihong Wu Zhicong Li Ruoshu Tang Hongtao Li Yinyin Huang Tianqi Wang Shaofen Xu Haoyu Cheng Zhitong Ye Dong Xiao Xiaolin Lin Gang Wu Richard T Jaspers Janak L Pathak |
| author_sort | Zhichao Zheng |
| collection | DOAJ |
| description | MicroRNA-155 (miR155) is overexpressed in various inflammatory diseases and cancer, in which bone resorption and osteolysis are frequently observed. However, the role of miR155 on osteogenesis and bone mass phenotype is still unknown. Here, we report a low bone mass phenotype in the long bone of Mir155-Tg mice compared with wild-type mice. In contrast, Mir155-KO mice showed a high bone mass phenotype and protective effect against inflammation-induced bone loss. Mir155-KO mice showed robust bone regeneration in the ectopic and orthotopic model, but Mir155-Tg mice showed compromised bone regeneration compared with the wild-type mice. Similarly, the osteogenic differentiation potential of bone marrow stromal stem cells (BMSCs) from Mir155-KO mice was robust and Mir155-Tg was compromised compared with that of wild-type mice. Moreover, Mir155 knockdown in BMSCs from wild-type mice showed higher osteogenic differentiation potential, supporting the results from Mir155-KO mice. TargetScan analysis predicted sphingosine 1-phosphate receptor-1 (S1pr1) as a target gene of Mir155, which was further confirmed by luciferase assay and Mir155 knockdown. S1pr1 overexpression in BMSCs robustly promoted osteogenic differentiation without affecting cell viability and proliferation. Furthermore, osteoclastogenic differentiation of Mir155-Tg bone marrow-derived macrophages was inhibited compared with that of wild-type mice. Thus, Mir155 showed a catabolic effect on osteogenesis and bone mass phenotype via interaction with the S1pr1 gene, suggesting inhibition of Mir155 as a potential strategy for bone regeneration and bone defect healing. |
| first_indexed | 2024-04-10T23:03:52Z |
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| id | doaj.art-c9d1c01966474a4094cd8d3dd4343316 |
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| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-10T23:03:52Z |
| publishDate | 2023-01-01 |
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| series | eLife |
| spelling | doaj.art-c9d1c01966474a4094cd8d3dd43433162023-01-13T16:37:56ZengeLife Sciences Publications LtdeLife2050-084X2023-01-011210.7554/eLife.77742Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 geneZhichao Zheng0Lihong Wu1https://orcid.org/0000-0002-4561-9400Zhicong Li2Ruoshu Tang3Hongtao Li4Yinyin Huang5Tianqi Wang6Shaofen Xu7Haoyu Cheng8Zhitong Ye9Dong Xiao10Xiaolin Lin11Gang Wu12Richard T Jaspers13https://orcid.org/0000-0002-6951-0952Janak L Pathak14https://orcid.org/0000-0003-2576-443XAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China; Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, NetherlandsAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Cancer Immunotherapy Research and Guangzhou Key Laboratory of Tumour Immunology Research, Cancer Research Institute, School of Basic Medical Science, Southern Medical University, Guangzhou, China; Institute of Comparative Medicine & Laboratory Animal Center, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Cancer Immunotherapy Research and Guangzhou Key Laboratory of Tumour Immunology Research, Cancer Research Institute, School of Basic Medical Science, Southern Medical University, Guangzhou, China; Institute of Comparative Medicine & Laboratory Animal Center, Southern Medical University, Guangzhou, ChinaDepartment of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Amsterdam Movement Science, Vrije Universiteit Amsterdam, Amsterdam, Netherlands; Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China; Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, NetherlandsAffiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, ChinaMicroRNA-155 (miR155) is overexpressed in various inflammatory diseases and cancer, in which bone resorption and osteolysis are frequently observed. However, the role of miR155 on osteogenesis and bone mass phenotype is still unknown. Here, we report a low bone mass phenotype in the long bone of Mir155-Tg mice compared with wild-type mice. In contrast, Mir155-KO mice showed a high bone mass phenotype and protective effect against inflammation-induced bone loss. Mir155-KO mice showed robust bone regeneration in the ectopic and orthotopic model, but Mir155-Tg mice showed compromised bone regeneration compared with the wild-type mice. Similarly, the osteogenic differentiation potential of bone marrow stromal stem cells (BMSCs) from Mir155-KO mice was robust and Mir155-Tg was compromised compared with that of wild-type mice. Moreover, Mir155 knockdown in BMSCs from wild-type mice showed higher osteogenic differentiation potential, supporting the results from Mir155-KO mice. TargetScan analysis predicted sphingosine 1-phosphate receptor-1 (S1pr1) as a target gene of Mir155, which was further confirmed by luciferase assay and Mir155 knockdown. S1pr1 overexpression in BMSCs robustly promoted osteogenic differentiation without affecting cell viability and proliferation. Furthermore, osteoclastogenic differentiation of Mir155-Tg bone marrow-derived macrophages was inhibited compared with that of wild-type mice. Thus, Mir155 showed a catabolic effect on osteogenesis and bone mass phenotype via interaction with the S1pr1 gene, suggesting inhibition of Mir155 as a potential strategy for bone regeneration and bone defect healing.https://elifesciences.org/articles/77742miR155bone massosteogenesisS1PR1cell viability |
| spellingShingle | Zhichao Zheng Lihong Wu Zhicong Li Ruoshu Tang Hongtao Li Yinyin Huang Tianqi Wang Shaofen Xu Haoyu Cheng Zhitong Ye Dong Xiao Xiaolin Lin Gang Wu Richard T Jaspers Janak L Pathak Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene eLife miR155 bone mass osteogenesis S1PR1 cell viability |
| title | Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene |
| title_full | Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene |
| title_fullStr | Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene |
| title_full_unstemmed | Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene |
| title_short | Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene |
| title_sort | mir155 regulates osteogenesis and bone mass phenotype via targeting s1pr1 gene |
| topic | miR155 bone mass osteogenesis S1PR1 cell viability |
| url | https://elifesciences.org/articles/77742 |
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