Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
Inflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment in...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9127 |
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| author | Inês Silva Priscila Mendes Sofia Guerra Rui Pinto Vanessa Mateus |
| author_facet | Inês Silva Priscila Mendes Sofia Guerra Rui Pinto Vanessa Mateus |
| author_sort | Inês Silva |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment includes drugs that induce and keep the patient in remission, not reversing the underlying pathogenic mechanism. These therapies, in the long term, may cause various side effects and complications, which has increased the need to investigate new, more effective, and safer pharmacological approaches. In preclinical studies, topiramate has demonstrated a potential anti-inflammatory effect by inhibiting the production of several pro-inflammatory cytokines. This study aimed to investigate the effect of topiramate in a chronic TNBS-induced colitis model in rodents. Experimental colitis was induced by four intrarectal administrations of 1% TNBS in female CD-1 mice. Topiramate 10 and 20 mg were administered intraperitoneally for 14 days. Several parameters were evaluated, such as bodyweight, alkaline phosphatase (ALP), fecal hemoglobin, fecal calprotectin, tumor necrosis factor (TNF)-α, and interleukin (IL)-10. Topiramate reduces TNBS-induced colonic damage in a model of chronic experimental colitis and normalizes the stool consistency and anus appearance. Additionally, topiramate significantly reduced the concentration of ALP, fecal hemoglobin, fecal calprotectin, TNF-α, and IL-10, demonstrating it to be a promising pharmacological approach for the treatment of IBD in the future. |
| first_indexed | 2024-03-09T04:20:23Z |
| format | Article |
| id | doaj.art-c9d1c266af664dc9b2dfe656902f79be |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:20:23Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c9d1c266af664dc9b2dfe656902f79be2023-12-03T13:48:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316912710.3390/ijms23169127Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced ColitisInês Silva0Priscila Mendes1Sofia Guerra2Rui Pinto3Vanessa Mateus4H&TRC–Health and Technology Research Center, ESTeSL–Lisbon School of Health and Technology, Instituto Politécnico de Lisboa, 1990-096 Lisbon, PortugalH&TRC–Health and Technology Research Center, ESTeSL–Lisbon School of Health and Technology, Instituto Politécnico de Lisboa, 1990-096 Lisbon, PortugalH&TRC–Health and Technology Research Center, ESTeSL–Lisbon School of Health and Technology, Instituto Politécnico de Lisboa, 1990-096 Lisbon, PortugaliMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, 1990-096 Lisbon, PortugalH&TRC–Health and Technology Research Center, ESTeSL–Lisbon School of Health and Technology, Instituto Politécnico de Lisboa, 1990-096 Lisbon, PortugalInflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment includes drugs that induce and keep the patient in remission, not reversing the underlying pathogenic mechanism. These therapies, in the long term, may cause various side effects and complications, which has increased the need to investigate new, more effective, and safer pharmacological approaches. In preclinical studies, topiramate has demonstrated a potential anti-inflammatory effect by inhibiting the production of several pro-inflammatory cytokines. This study aimed to investigate the effect of topiramate in a chronic TNBS-induced colitis model in rodents. Experimental colitis was induced by four intrarectal administrations of 1% TNBS in female CD-1 mice. Topiramate 10 and 20 mg were administered intraperitoneally for 14 days. Several parameters were evaluated, such as bodyweight, alkaline phosphatase (ALP), fecal hemoglobin, fecal calprotectin, tumor necrosis factor (TNF)-α, and interleukin (IL)-10. Topiramate reduces TNBS-induced colonic damage in a model of chronic experimental colitis and normalizes the stool consistency and anus appearance. Additionally, topiramate significantly reduced the concentration of ALP, fecal hemoglobin, fecal calprotectin, TNF-α, and IL-10, demonstrating it to be a promising pharmacological approach for the treatment of IBD in the future.https://www.mdpi.com/1422-0067/23/16/9127inflammatory bowel diseaseTNBS-induced colitistopiramateanti-inflammatory effectexperimental colitis model |
| spellingShingle | Inês Silva Priscila Mendes Sofia Guerra Rui Pinto Vanessa Mateus Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis International Journal of Molecular Sciences inflammatory bowel disease TNBS-induced colitis topiramate anti-inflammatory effect experimental colitis model |
| title | Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis |
| title_full | Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis |
| title_fullStr | Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis |
| title_full_unstemmed | Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis |
| title_short | Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis |
| title_sort | anti inflammatory effect of topiramate in a chronic model of tnbs induced colitis |
| topic | inflammatory bowel disease TNBS-induced colitis topiramate anti-inflammatory effect experimental colitis model |
| url | https://www.mdpi.com/1422-0067/23/16/9127 |
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