The preparative isolation of lecithin.

Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica...

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Main Author: N S Radin
Format: Article
Language:English
Published: Elsevier 1978-09-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520407084
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author N S Radin
author_facet N S Radin
author_sort N S Radin
collection DOAJ
description Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cyclohexane. Toxic solvents (chloroform and methanol) are avoided by the use of ethanol, isopropanol, hexane, and water. The elutions are easily monitored by a flow cell in an ordinary spectrophotometer set at 215 nm. Study of the column parameters has made it possible to use heavy loads with a relatively small column and minimal solvent.
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spelling doaj.art-c9d587c61cb64365badc522d00eda48c2022-12-21T21:58:28ZengElsevierJournal of Lipid Research0022-22751978-09-01197922924The preparative isolation of lecithin.N S RadinLecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cyclohexane. Toxic solvents (chloroform and methanol) are avoided by the use of ethanol, isopropanol, hexane, and water. The elutions are easily monitored by a flow cell in an ordinary spectrophotometer set at 215 nm. Study of the column parameters has made it possible to use heavy loads with a relatively small column and minimal solvent.http://www.sciencedirect.com/science/article/pii/S0022227520407084
spellingShingle N S Radin
The preparative isolation of lecithin.
Journal of Lipid Research
title The preparative isolation of lecithin.
title_full The preparative isolation of lecithin.
title_fullStr The preparative isolation of lecithin.
title_full_unstemmed The preparative isolation of lecithin.
title_short The preparative isolation of lecithin.
title_sort preparative isolation of lecithin
url http://www.sciencedirect.com/science/article/pii/S0022227520407084
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