The preparative isolation of lecithin.
Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica...
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Format: | Article |
Language: | English |
Published: |
Elsevier
1978-09-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520407084 |
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author | N S Radin |
author_facet | N S Radin |
author_sort | N S Radin |
collection | DOAJ |
description | Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cyclohexane. Toxic solvents (chloroform and methanol) are avoided by the use of ethanol, isopropanol, hexane, and water. The elutions are easily monitored by a flow cell in an ordinary spectrophotometer set at 215 nm. Study of the column parameters has made it possible to use heavy loads with a relatively small column and minimal solvent. |
first_indexed | 2024-12-17T07:32:04Z |
format | Article |
id | doaj.art-c9d587c61cb64365badc522d00eda48c |
institution | Directory Open Access Journal |
issn | 0022-2275 |
language | English |
last_indexed | 2024-12-17T07:32:04Z |
publishDate | 1978-09-01 |
publisher | Elsevier |
record_format | Article |
series | Journal of Lipid Research |
spelling | doaj.art-c9d587c61cb64365badc522d00eda48c2022-12-21T21:58:28ZengElsevierJournal of Lipid Research0022-22751978-09-01197922924The preparative isolation of lecithin.N S RadinLecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cyclohexane. Toxic solvents (chloroform and methanol) are avoided by the use of ethanol, isopropanol, hexane, and water. The elutions are easily monitored by a flow cell in an ordinary spectrophotometer set at 215 nm. Study of the column parameters has made it possible to use heavy loads with a relatively small column and minimal solvent.http://www.sciencedirect.com/science/article/pii/S0022227520407084 |
spellingShingle | N S Radin The preparative isolation of lecithin. Journal of Lipid Research |
title | The preparative isolation of lecithin. |
title_full | The preparative isolation of lecithin. |
title_fullStr | The preparative isolation of lecithin. |
title_full_unstemmed | The preparative isolation of lecithin. |
title_short | The preparative isolation of lecithin. |
title_sort | preparative isolation of lecithin |
url | http://www.sciencedirect.com/science/article/pii/S0022227520407084 |
work_keys_str_mv | AT nsradin thepreparativeisolationoflecithin AT nsradin preparativeisolationoflecithin |