Distinguishing grapefruit and pummelo accessions using ISSR markers
Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka acce...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2010-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0005_distinguishing-grapefruit-and-pummelo-accessions-using-issr-markers.php |
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| author | Aydin Uzun Osman Gulsen Turgut Yesiloglu Yildiz Aka-Kacar Onder Tuzcu |
| author_facet | Aydin Uzun Osman Gulsen Turgut Yesiloglu Yildiz Aka-Kacar Onder Tuzcu |
| author_sort | Aydin Uzun |
| collection | DOAJ |
| description | Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The number of average polymorphic fragments per primer was 5.2. The mean polymorphism information content (PIC) was 0.37. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity range from 0.79 to 1.00. The accessions were separated into two main clusters; group A with five pummelos and group B with grapefruits. In the pummelo cluster, all pummelos were distinguished whereas in the grapefruit cluster some accessions were not clearly separated. There was a low level of variation in the grapefruits due to their mutation origin. |
| first_indexed | 2024-04-10T08:29:48Z |
| format | Article |
| id | doaj.art-c9d7e7f3085f40099e5b19cfab999b8b |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:48Z |
| publishDate | 2010-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-c9d7e7f3085f40099e5b19cfab999b8b2023-02-23T03:30:07ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252010-12-0146417017710.17221/89/2010-CJGPBcjg-201004-0005Distinguishing grapefruit and pummelo accessions using ISSR markersAydin Uzun0Osman Gulsen1Turgut Yesiloglu2Yildiz Aka-Kacar3Onder Tuzcu4Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, TurkeyDepartment of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, TurkeyDepartment of Horticulture, Faculty of Agriculture, Cukurova University, Adana, TurkeyDepartment of Horticulture, Faculty of Agriculture, Cukurova University, Adana, TurkeyDepartment of Horticulture, Faculty of Agriculture, Cukurova University, Adana, TurkeyGrapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The number of average polymorphic fragments per primer was 5.2. The mean polymorphism information content (PIC) was 0.37. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity range from 0.79 to 1.00. The accessions were separated into two main clusters; group A with five pummelos and group B with grapefruits. In the pummelo cluster, all pummelos were distinguished whereas in the grapefruit cluster some accessions were not clearly separated. There was a low level of variation in the grapefruits due to their mutation origin.https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0005_distinguishing-grapefruit-and-pummelo-accessions-using-issr-markers.phpaccessioncitrus maximacitrus paradisigermplasm characterizationmolecular markers |
| spellingShingle | Aydin Uzun Osman Gulsen Turgut Yesiloglu Yildiz Aka-Kacar Onder Tuzcu Distinguishing grapefruit and pummelo accessions using ISSR markers Czech Journal of Genetics and Plant Breeding accession citrus maxima citrus paradisi germplasm characterization molecular markers |
| title | Distinguishing grapefruit and pummelo accessions using ISSR markers |
| title_full | Distinguishing grapefruit and pummelo accessions using ISSR markers |
| title_fullStr | Distinguishing grapefruit and pummelo accessions using ISSR markers |
| title_full_unstemmed | Distinguishing grapefruit and pummelo accessions using ISSR markers |
| title_short | Distinguishing grapefruit and pummelo accessions using ISSR markers |
| title_sort | distinguishing grapefruit and pummelo accessions using issr markers |
| topic | accession citrus maxima citrus paradisi germplasm characterization molecular markers |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0005_distinguishing-grapefruit-and-pummelo-accessions-using-issr-markers.php |
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