Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet
Background and aimsNonalcoholic fatty liver disease (NAFLD) is the most common type of chronic liver disease with a high incidence, and the situation is not optimistic. Intestinal flora imbalance is strongly correlated with NAFLD pathogenesis. Zhishi Daozhi Decoction (ZDD) is a water decoction of th...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1005318/full |
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| author | Chao-Ran Bi Jia-Tong Sun Jian Du Li-Yuan Chu Yi-Jing Li Xiao-Yu Jia Yuan Liu Wen-Ping Zhang Yu-Chun Li Yan-Jing Liu |
| author_facet | Chao-Ran Bi Jia-Tong Sun Jian Du Li-Yuan Chu Yi-Jing Li Xiao-Yu Jia Yuan Liu Wen-Ping Zhang Yu-Chun Li Yan-Jing Liu |
| author_sort | Chao-Ran Bi |
| collection | DOAJ |
| description | Background and aimsNonalcoholic fatty liver disease (NAFLD) is the most common type of chronic liver disease with a high incidence, and the situation is not optimistic. Intestinal flora imbalance is strongly correlated with NAFLD pathogenesis. Zhishi Daozhi Decoction (ZDD) is a water decoction of the herbs used in the classical Chinese medicine prescription Zhishi Daozhi Pills. Zhishi Daozhi Pills has shown promising hepatoprotective and hypolipidemic properties, but its specific mechanism remains unclear.MethodsMice were fed on a high fat-rich diet (HFD) for ten weeks, and then the animals were administrated ZDD through oral gavage for four weeks. The serum liver function and blood lipid indexes of the mice were then tested using an automatic biochemical analyzer. H&E and Oil Red O staining were used to observe the pathological conditions of mice liver tissue, and 16S rRNA sequencing technology was used to analyze the changes in intestinal flora of mice. The concentration of short-chain fatty acids (SCFAs) in the gut of mice was analyzed by gas chromatography-mass spectrometry (GC-MS). The expression of tight junction (TJ) proteins between ileal mucosal epithelial cells was analyzed using the immunofluorescence technique.ResultsZDD was found to reduce the bodyweight of NAFLD mice, reduce serum TG, CHO, ALT, and AST levels, reduce fat accumulation in liver tissue, make the structure of intestinal flora comparable to the control group, and increase the concentration of intestinal SCFAs. It was also found to increase the expression of TJ proteins such as occludin and ZO-1, making them comparable to the control group.ConclusionsZDD has a therapeutic effect on NAFLD mice induced by HFD, which may act by optimizing the intestinal flora structure. |
| first_indexed | 2024-04-11T00:59:11Z |
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| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T00:59:11Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c9dda7836cd440d0b34ceb72aecc1b1a2023-01-04T19:41:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10053181005318Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat dietChao-Ran Bi0Jia-Tong Sun1Jian Du2Li-Yuan Chu3Yi-Jing Li4Xiao-Yu Jia5Yuan Liu6Wen-Ping Zhang7Yu-Chun Li8Yan-Jing Liu9College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaDepartment of Endocrinology, Metabolism and Gastroenterology, Third Affiliated Clinical Hospital of Changchun University of Chinese Medicine, Changchun, ChinaDepartment of Ophthalmology, China-Japan Friendship Hospital of Jilin University, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaDepartment of Spleen and Gastroenterology, Jilin Provincial Academy of Traditional Chinese Medicine, Changchun, ChinaDepartment of Endocrinology, Metabolism and Gastroenterology, Third Affiliated Clinical Hospital of Changchun University of Chinese Medicine, Changchun, ChinaBackground and aimsNonalcoholic fatty liver disease (NAFLD) is the most common type of chronic liver disease with a high incidence, and the situation is not optimistic. Intestinal flora imbalance is strongly correlated with NAFLD pathogenesis. Zhishi Daozhi Decoction (ZDD) is a water decoction of the herbs used in the classical Chinese medicine prescription Zhishi Daozhi Pills. Zhishi Daozhi Pills has shown promising hepatoprotective and hypolipidemic properties, but its specific mechanism remains unclear.MethodsMice were fed on a high fat-rich diet (HFD) for ten weeks, and then the animals were administrated ZDD through oral gavage for four weeks. The serum liver function and blood lipid indexes of the mice were then tested using an automatic biochemical analyzer. H&E and Oil Red O staining were used to observe the pathological conditions of mice liver tissue, and 16S rRNA sequencing technology was used to analyze the changes in intestinal flora of mice. The concentration of short-chain fatty acids (SCFAs) in the gut of mice was analyzed by gas chromatography-mass spectrometry (GC-MS). The expression of tight junction (TJ) proteins between ileal mucosal epithelial cells was analyzed using the immunofluorescence technique.ResultsZDD was found to reduce the bodyweight of NAFLD mice, reduce serum TG, CHO, ALT, and AST levels, reduce fat accumulation in liver tissue, make the structure of intestinal flora comparable to the control group, and increase the concentration of intestinal SCFAs. It was also found to increase the expression of TJ proteins such as occludin and ZO-1, making them comparable to the control group.ConclusionsZDD has a therapeutic effect on NAFLD mice induced by HFD, which may act by optimizing the intestinal flora structure.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1005318/fullnonalcoholic fatty liver diseaseintestinal floraZhishi Daozhi Decoctionhigh-fat diettraditional Chinese medicine |
| spellingShingle | Chao-Ran Bi Jia-Tong Sun Jian Du Li-Yuan Chu Yi-Jing Li Xiao-Yu Jia Yuan Liu Wen-Ping Zhang Yu-Chun Li Yan-Jing Liu Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet Frontiers in Cellular and Infection Microbiology nonalcoholic fatty liver disease intestinal flora Zhishi Daozhi Decoction high-fat diet traditional Chinese medicine |
| title | Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet |
| title_full | Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet |
| title_fullStr | Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet |
| title_full_unstemmed | Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet |
| title_short | Effects of Zhishi Daozhi Decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high-fat diet |
| title_sort | effects of zhishi daozhi decoction on the intestinal flora of nonalcoholic fatty liver disease mice induced by a high fat diet |
| topic | nonalcoholic fatty liver disease intestinal flora Zhishi Daozhi Decoction high-fat diet traditional Chinese medicine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1005318/full |
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