Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics
Abstract Renal pelvis dilatation (RPD) is diagnosed in utero on prenatal ultrasonography (US) and can resolve spontaneously. However, isolated RPD can also reflect ureteropelvic junction obstruction (UPJO), which requires surgical treatment to prevent progressive renal deterioration. The diagnosis o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17664-4 |
| _version_ | 1811342739309068288 |
|---|---|
| author | Aurélien Scalabre Yohann Clément Florence Guillière Sophie Ayciriex Ségolène Gaillard Delphine Demède Aurore Bouty Pierre Lanteri Pierre-Yves Mure |
| author_facet | Aurélien Scalabre Yohann Clément Florence Guillière Sophie Ayciriex Ségolène Gaillard Delphine Demède Aurore Bouty Pierre Lanteri Pierre-Yves Mure |
| author_sort | Aurélien Scalabre |
| collection | DOAJ |
| description | Abstract Renal pelvis dilatation (RPD) is diagnosed in utero on prenatal ultrasonography (US) and can resolve spontaneously. However, isolated RPD can also reflect ureteropelvic junction obstruction (UPJO), which requires surgical treatment to prevent progressive renal deterioration. The diagnosis of UPJO can only be confirmed after birth with repeat US and renal isotope studies. 1H Nuclear Magnetic Resonance spectroscopy (NMR) was performed on urine of newborns with prenatally diagnosed unilateral RPD and healthy controls to identify specific urinary biomarkers for UPJO. The original combination of EigenMS normalization and sparse partial-least-squares discriminant analysis improved selectivity and sensitivity. In total, 140 urine samples from newborns were processed and 100 metabolites were identified. Correlation network identified discriminant metabolites in lower concentrations in UPJO patients. Two main metabolic pathways appeared to be impaired in patients with UPJO i.e. amino acid and betaine metabolism. In this prospective study, metabolic profiling of urine samples by NMR clearly distinguishes patients who required surgery for UPJO from patients with transient dilatations and controls. This study will pave the way for the use of metabolomics for the diagnosis of prenatal hydronephrosis in clinical routine. |
| first_indexed | 2024-04-13T19:16:06Z |
| format | Article |
| id | doaj.art-c9de9d39e5d04a859ea6f840063c62ea |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T19:16:06Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c9de9d39e5d04a859ea6f840063c62ea2022-12-22T02:33:40ZengNature PortfolioScientific Reports2045-23222022-08-011211910.1038/s41598-022-17664-4Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomicsAurélien Scalabre0Yohann Clément1Florence Guillière2Sophie Ayciriex3Ségolène Gaillard4Delphine Demède5Aurore Bouty6Pierre Lanteri7Pierre-Yves Mure8CHU Nord de Saint-Etienne, Service de chirurgie pédiatriqueUniv Lyon, CNRS, Université Claude Bernard Lyon 1, Institut Des Sciences Analytiques, UMR 5280Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut Des Sciences Analytiques, UMR 5280Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut Des Sciences Analytiques, UMR 5280Hospices Civils de Lyon, Inserm, EPICIME-CIC 1407Hospices Civils de Lyon, Service de chirurgie pédiatrique, Hôpital Femme Mère EnfantHospices Civils de Lyon, Service de chirurgie pédiatrique, Hôpital Femme Mère EnfantUniv Lyon, CNRS, Université Claude Bernard Lyon 1, Institut Des Sciences Analytiques, UMR 5280Hospices Civils de Lyon, Service de chirurgie pédiatrique, Hôpital Femme Mère EnfantAbstract Renal pelvis dilatation (RPD) is diagnosed in utero on prenatal ultrasonography (US) and can resolve spontaneously. However, isolated RPD can also reflect ureteropelvic junction obstruction (UPJO), which requires surgical treatment to prevent progressive renal deterioration. The diagnosis of UPJO can only be confirmed after birth with repeat US and renal isotope studies. 1H Nuclear Magnetic Resonance spectroscopy (NMR) was performed on urine of newborns with prenatally diagnosed unilateral RPD and healthy controls to identify specific urinary biomarkers for UPJO. The original combination of EigenMS normalization and sparse partial-least-squares discriminant analysis improved selectivity and sensitivity. In total, 140 urine samples from newborns were processed and 100 metabolites were identified. Correlation network identified discriminant metabolites in lower concentrations in UPJO patients. Two main metabolic pathways appeared to be impaired in patients with UPJO i.e. amino acid and betaine metabolism. In this prospective study, metabolic profiling of urine samples by NMR clearly distinguishes patients who required surgery for UPJO from patients with transient dilatations and controls. This study will pave the way for the use of metabolomics for the diagnosis of prenatal hydronephrosis in clinical routine.https://doi.org/10.1038/s41598-022-17664-4 |
| spellingShingle | Aurélien Scalabre Yohann Clément Florence Guillière Sophie Ayciriex Ségolène Gaillard Delphine Demède Aurore Bouty Pierre Lanteri Pierre-Yves Mure Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics Scientific Reports |
| title | Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics |
| title_full | Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics |
| title_fullStr | Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics |
| title_full_unstemmed | Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics |
| title_short | Early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1H NMR urinary metabolomics |
| title_sort | early detection of ureteropelvic junction obstruction in neonates with prenatal diagnosis of renal pelvis dilatation using 1h nmr urinary metabolomics |
| url | https://doi.org/10.1038/s41598-022-17664-4 |
| work_keys_str_mv | AT aurelienscalabre earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT yohannclement earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT florenceguilliere earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT sophieayciriex earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT segolenegaillard earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT delphinedemede earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT aurorebouty earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT pierrelanteri earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics AT pierreyvesmure earlydetectionofureteropelvicjunctionobstructioninneonateswithprenataldiagnosisofrenalpelvisdilatationusing1hnmrurinarymetabolomics |