Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch
The deciduous conifer larch has been widely distributed around the world, is a high-quality wood species and is also used to extract industrial raw materials and medicines. In this study, we developed an organogenesis protocol for Larix olgensis from both mature zygotic embryos and needles, and anal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2023-01-01
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| Series: | Forestry Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/FR-2023-0004 |
| _version_ | 1797293190651314176 |
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| author | Xuetong Yan Kejia Wang Keyuan Zheng Lifeng Zhang Yang Ye Liwang Qi Mulan Zhu |
| author_facet | Xuetong Yan Kejia Wang Keyuan Zheng Lifeng Zhang Yang Ye Liwang Qi Mulan Zhu |
| author_sort | Xuetong Yan |
| collection | DOAJ |
| description | The deciduous conifer larch has been widely distributed around the world, is a high-quality wood species and is also used to extract industrial raw materials and medicines. In this study, we developed an organogenesis protocol for Larix olgensis from both mature zygotic embryos and needles, and analyzed the content of taxifolin in different tissues. The highest callus induction (96.8%) from mature zygotic embryo was found in the Douglas-fir Cotyledon Revised (DCR) medium augmented with 2.0 mg·L−1 6-Benzylaminopurine (6-BA) and 0.2 mg·L−1 α-Naphthaleneacetic acid (NAA), while from needles the highest callus induction (92.03%) was found in the Murashige and Skoog (MS) medium augmented with 3 mg·L−1 6-BA and 0.3 mg·L−1 NAA. The best shoot regeneration capacity from zygotic embryo-derived calli (83.3%) was obtained in DCR medium augmented with 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA, and needle-derived calli were 77.3%. The shoots achieved the highest elongation (75.6%) in the DCR medium supplemented with 0.5 mg·L−1 6-BA, 0.05 mg·L−1 NAA and 2 g·L−1 activated charcoal (AC). The rooting rate was 62.8% in DCR medium augmented with 3 mg·L−1 Indole-3-butyric acid (IBA) and 100 mg·L−1 phloroglucinol (PG). The accumulation of the taxifolin in elongation shoots and lignified elongation shoots have greatly improved along with the development process, were 28.6 µg·g−1, and 53 µg·g−1 respectively. The content of the taxifolin in callus was 1.99−5.26 µg·g−1, adventitious shoots were 4.8 µg·g−1, and adventitious roots were 2.86 µg·g−1. We report an efficient organogenesis and taxifolin production protocol in larch for the first time. |
| first_indexed | 2024-03-07T20:09:18Z |
| format | Article |
| id | doaj.art-c9e60f1139354693898c53430b3470f8 |
| institution | Directory Open Access Journal |
| issn | 2767-3812 |
| language | English |
| last_indexed | 2024-03-07T20:09:18Z |
| publishDate | 2023-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Forestry Research |
| spelling | doaj.art-c9e60f1139354693898c53430b3470f82024-02-28T01:55:38ZengMaximum Academic PressForestry Research2767-38122023-01-01311910.48130/FR-2023-0004FR-2023-0004Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larchXuetong Yan0Kejia Wang1Keyuan Zheng2Lifeng Zhang3Yang Ye4Liwang Qi5Mulan Zhu6National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai 200032, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Drug Research and Department of Natural Products Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaNational Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai 200032, ChinaThe deciduous conifer larch has been widely distributed around the world, is a high-quality wood species and is also used to extract industrial raw materials and medicines. In this study, we developed an organogenesis protocol for Larix olgensis from both mature zygotic embryos and needles, and analyzed the content of taxifolin in different tissues. The highest callus induction (96.8%) from mature zygotic embryo was found in the Douglas-fir Cotyledon Revised (DCR) medium augmented with 2.0 mg·L−1 6-Benzylaminopurine (6-BA) and 0.2 mg·L−1 α-Naphthaleneacetic acid (NAA), while from needles the highest callus induction (92.03%) was found in the Murashige and Skoog (MS) medium augmented with 3 mg·L−1 6-BA and 0.3 mg·L−1 NAA. The best shoot regeneration capacity from zygotic embryo-derived calli (83.3%) was obtained in DCR medium augmented with 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA, and needle-derived calli were 77.3%. The shoots achieved the highest elongation (75.6%) in the DCR medium supplemented with 0.5 mg·L−1 6-BA, 0.05 mg·L−1 NAA and 2 g·L−1 activated charcoal (AC). The rooting rate was 62.8% in DCR medium augmented with 3 mg·L−1 Indole-3-butyric acid (IBA) and 100 mg·L−1 phloroglucinol (PG). The accumulation of the taxifolin in elongation shoots and lignified elongation shoots have greatly improved along with the development process, were 28.6 µg·g−1, and 53 µg·g−1 respectively. The content of the taxifolin in callus was 1.99−5.26 µg·g−1, adventitious shoots were 4.8 µg·g−1, and adventitious roots were 2.86 µg·g−1. We report an efficient organogenesis and taxifolin production protocol in larch for the first time.https://www.maxapress.com/article/doi/10.48130/FR-2023-0004larix olgensismature zygotic embryoneedleorganogenesistaxifolin production |
| spellingShingle | Xuetong Yan Kejia Wang Keyuan Zheng Lifeng Zhang Yang Ye Liwang Qi Mulan Zhu Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch Forestry Research larix olgensis mature zygotic embryo needle organogenesis taxifolin production |
| title | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| title_full | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| title_fullStr | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| title_full_unstemmed | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| title_short | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| title_sort | efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch |
| topic | larix olgensis mature zygotic embryo needle organogenesis taxifolin production |
| url | https://www.maxapress.com/article/doi/10.48130/FR-2023-0004 |
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