Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice
Abstract UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function reg...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-023-03563-7 |
| _version_ | 1797578346821844992 |
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| author | Hu Jiajia Yang Ziyao Zheng Jiaqi Chen Yanli Zhao Xiaotao Su Ming |
| author_facet | Hu Jiajia Yang Ziyao Zheng Jiaqi Chen Yanli Zhao Xiaotao Su Ming |
| author_sort | Hu Jiajia |
| collection | DOAJ |
| description | Abstract UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMylation-associated gene. Overexpression of Ufm1 significantly upregulated the protein expression of Tnfaip2, whereas isoproterenol treatment decreased Tnfaip2 expression in Tg-Ufm1 mice. These data may provide novel clues for UFMylation in cardiac hypertrophy. |
| first_indexed | 2024-03-10T22:20:40Z |
| format | Article |
| id | doaj.art-c9e91a0866c24e56ba008cabd1d57bc3 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-03-10T22:20:40Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-c9e91a0866c24e56ba008cabd1d57bc32023-11-19T12:18:13ZengBMCBMC Cardiovascular Disorders1471-22612023-11-012311910.1186/s12872-023-03563-7Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic miceHu Jiajia0Yang Ziyao1Zheng Jiaqi2Chen Yanli3Zhao Xiaotao4Su Ming5Department of Clinical Laboratory, Peking University People’s HospitalDepartment of Blood Transfusion, Peking University First HospitalDepartment of Clinical Laboratory, Peking University People’s HospitalDepartment of Clinical Laboratory, Peking University People’s HospitalDepartment of Clinical Laboratory, Peking University People’s HospitalDepartment of Clinical Laboratory, Peking University People’s HospitalAbstract UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMylation-associated gene. Overexpression of Ufm1 significantly upregulated the protein expression of Tnfaip2, whereas isoproterenol treatment decreased Tnfaip2 expression in Tg-Ufm1 mice. These data may provide novel clues for UFMylation in cardiac hypertrophy.https://doi.org/10.1186/s12872-023-03563-7Ufm1UFMylationCardiac hypertrophyTnfaip2 |
| spellingShingle | Hu Jiajia Yang Ziyao Zheng Jiaqi Chen Yanli Zhao Xiaotao Su Ming Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice BMC Cardiovascular Disorders Ufm1 UFMylation Cardiac hypertrophy Tnfaip2 |
| title | Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
| title_full | Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
| title_fullStr | Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
| title_full_unstemmed | Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
| title_short | Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
| title_sort | screening ufmylation associated genes in heart tissues of ufm1 transgenic mice |
| topic | Ufm1 UFMylation Cardiac hypertrophy Tnfaip2 |
| url | https://doi.org/10.1186/s12872-023-03563-7 |
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