Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes
<p>Abstract</p> <p>Background</p> <p>MicroRNAs play vital role in plant growth and development by changeable expression of their target genes with most plant microRNAs having perfect or near-perfect complementarities with their target genes but miRNAs in <it>Citru...
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | BMC Research Notes |
| Online Access: | http://www.biomedcentral.com/1756-0500/5/235 |
| _version_ | 1831614275284631552 |
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| author | Song Changnian Yu Mingliang Han Jian Wang Chen Liu Hong Zhang Yanping Fang Jinggui |
| author_facet | Song Changnian Yu Mingliang Han Jian Wang Chen Liu Hong Zhang Yanping Fang Jinggui |
| author_sort | Song Changnian |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>MicroRNAs play vital role in plant growth and development by changeable expression of their target genes with most plant microRNAs having perfect or near-perfect complementarities with their target genes but miRNAs in <it>Citrus sinensis</it> (csi-miRNAs) and their function have not been widely studied.</p> <p>Findings</p> <p>In this study, 15 potential microRNAs in <it>Citrus sinensis</it> (csi-miRNAs) were identified and bioinformatically validated using miR-RACE, a newly developed method for determination of miRNAs prediction computationally. The expression of these fifteen <it>C. sinensis</it> miRNAs can be detected in leaves, stems, flowers and fruits of <it>C. sinensis</it> by QRT-PCR with some of them showed tissue-specific expression. Six potential target genes were identified for six csi-miRNAs and also experimentally verified by Poly (A) polymerase -mediated 3′ rapid amplification of cDNA ends (PPM-RACE) and RNA ligase-mediated 5′ rapid amplification of cDNA ends (RLM-RACE) which mapped the cleavage site of target mRNAs and detected expression patterns of cleaved fragments that indicate the regulatory function of the miRNAs on their target genes.</p> <p>Conclusions</p> <p>Our results confirm that small RNA-mediated regulation whereby all csi-miRNAs regulate their target genes by degradation.</p> |
| first_indexed | 2024-12-18T19:24:58Z |
| format | Article |
| id | doaj.art-c9ea9d37903c4b2d85783f87988f3e51 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-18T19:24:58Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-c9ea9d37903c4b2d85783f87988f3e512022-12-21T20:55:53ZengBMCBMC Research Notes1756-05002012-05-015123510.1186/1756-0500-5-235Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genesSong ChangnianYu MingliangHan JianWang ChenLiu HongZhang YanpingFang Jinggui<p>Abstract</p> <p>Background</p> <p>MicroRNAs play vital role in plant growth and development by changeable expression of their target genes with most plant microRNAs having perfect or near-perfect complementarities with their target genes but miRNAs in <it>Citrus sinensis</it> (csi-miRNAs) and their function have not been widely studied.</p> <p>Findings</p> <p>In this study, 15 potential microRNAs in <it>Citrus sinensis</it> (csi-miRNAs) were identified and bioinformatically validated using miR-RACE, a newly developed method for determination of miRNAs prediction computationally. The expression of these fifteen <it>C. sinensis</it> miRNAs can be detected in leaves, stems, flowers and fruits of <it>C. sinensis</it> by QRT-PCR with some of them showed tissue-specific expression. Six potential target genes were identified for six csi-miRNAs and also experimentally verified by Poly (A) polymerase -mediated 3′ rapid amplification of cDNA ends (PPM-RACE) and RNA ligase-mediated 5′ rapid amplification of cDNA ends (RLM-RACE) which mapped the cleavage site of target mRNAs and detected expression patterns of cleaved fragments that indicate the regulatory function of the miRNAs on their target genes.</p> <p>Conclusions</p> <p>Our results confirm that small RNA-mediated regulation whereby all csi-miRNAs regulate their target genes by degradation.</p>http://www.biomedcentral.com/1756-0500/5/235 |
| spellingShingle | Song Changnian Yu Mingliang Han Jian Wang Chen Liu Hong Zhang Yanping Fang Jinggui Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes BMC Research Notes |
| title | Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes |
| title_full | Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes |
| title_fullStr | Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes |
| title_full_unstemmed | Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes |
| title_short | Validation and characterization of <it>Citrus sinensis</it> microRNAs and their target genes |
| title_sort | validation and characterization of it citrus sinensis it micrornas and their target genes |
| url | http://www.biomedcentral.com/1756-0500/5/235 |
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