Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal
The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1,...
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Asian-Australasian Association of Animal Production Societies
2016-03-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-29-3-321.pdf |
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| author | Nu-Ri Choi Dong-Won Seo Ki-Myung Choi Na-Young Ko Ji-Ho Kim Hyun-Il Kim Woo-Young Jung Jun-Heon Lee |
| author_facet | Nu-Ri Choi Dong-Won Seo Ki-Myung Choi Na-Young Ko Ji-Ho Kim Hyun-Il Kim Woo-Young Jung Jun-Heon Lee |
| author_sort | Nu-Ri Choi |
| collection | DOAJ |
| description | The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research. |
| first_indexed | 2024-12-10T19:26:18Z |
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| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-10T19:26:18Z |
| publishDate | 2016-03-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-c9ee203145c143c5917509a7d42b1eab2022-12-22T01:36:21ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-03-0129332132610.5713/ajas.15.033123337Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model AnimalNu-Ri Choi0Dong-Won Seo1Ki-Myung Choi2Na-Young Ko3Ji-Ho Kim4Hyun-Il Kim5Woo-Young Jung6Jun-Heon Lee7 Optipharm Inc, Cheongju 28158, .Korea Optipharm Inc, Cheongju 28158, .Korea Optipharm Inc, Cheongju 28158, .Korea Optipharm Inc, Cheongju 28158, .Korea Optipharm Inc, Cheongju 28158, .Korea Optipharm Inc, Cheongju 28158, .Korea Hanwoo Improvement Center, National Agricultural Cooperative Federation, Seosan 31948, .Korea Optipharm Inc, Cheongju 28158, .KoreaThe porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research.http://www.ajas.info/upload/pdf/ajas-29-3-321.pdfHaplotypeMajor Histocompatibility Complex [MHC]Swine Leukocyte Antigen [SLA]Yucatan Miniature Pig |
| spellingShingle | Nu-Ri Choi Dong-Won Seo Ki-Myung Choi Na-Young Ko Ji-Ho Kim Hyun-Il Kim Woo-Young Jung Jun-Heon Lee Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal Asian-Australasian Journal of Animal Sciences Haplotype Major Histocompatibility Complex [MHC] Swine Leukocyte Antigen [SLA] Yucatan Miniature Pig |
| title | Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal |
| title_full | Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal |
| title_fullStr | Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal |
| title_full_unstemmed | Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal |
| title_short | Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal |
| title_sort | analysis of swine leukocyte antigen haplotypes in yucatan miniature pigs used as biomedical model animal |
| topic | Haplotype Major Histocompatibility Complex [MHC] Swine Leukocyte Antigen [SLA] Yucatan Miniature Pig |
| url | http://www.ajas.info/upload/pdf/ajas-29-3-321.pdf |
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