Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation
Cancer is one of the major causes of death worldwide. The repercussions of conventional therapeutic approaches present a challenge in the delivery of new effective treatments. Thus, more attention is being awarded to natural products, mainly honey. Honey could be the basis for the development of new...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/1/35 |
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| author | Nouha Bouali Walid Sabri Hamadou Riadh Badraoui Ramzi Hadj Lajimi Assia Hamdi Mousa Alreshidi Mohd Adnan Zohra Soua Arif Jamal Siddiqui Emira Noumi Mejdi Snoussi |
| author_facet | Nouha Bouali Walid Sabri Hamadou Riadh Badraoui Ramzi Hadj Lajimi Assia Hamdi Mousa Alreshidi Mohd Adnan Zohra Soua Arif Jamal Siddiqui Emira Noumi Mejdi Snoussi |
| author_sort | Nouha Bouali |
| collection | DOAJ |
| description | Cancer is one of the major causes of death worldwide. The repercussions of conventional therapeutic approaches present a challenge in the delivery of new effective treatments. Thus, more attention is being awarded to natural products, mainly honey. Honey could be the basis for the development of new therapies for cancer patients. The aim of this study is to assess the phytochemical profiling, antioxidant, drug-likeness properties, and anticancer activity of <i>Ziziphus</i> honey (ZH) derived from the Hail region of Saudi Arabia. The phytochemical profiling using high resolution-liquid chromatography mass spectrometry (HR-LCMS) revealed 10 compounds belonging to several familial classes and one tripeptide. Potential antioxidant activity was noted as assessed by DPPH (IC<sub>50</sub> 0.670 mg/mL), ABTS (IC<sub>50</sub> 3.554 mg/mL), and β-carotene (IC<sub>50</sub> > 5 mg/mL). The ZH exerted a notable cytotoxic effect in a dose-dependent manner against three cancer cell lines: lung (A549), breast (MCF-7), and colon (HCT-116), with respective IC<sub>50</sub> values of 5.203%, 6.02%, and 7.257%. The drug-likeness investigation unveiled that most of the identified compounds meet Lipinski’s rule. The molecular docking analysis revealed interesting antioxidant and anticancer activities for most targeted proteins and supported the in vitro findings. The Miraxanthin-III compound exhibited the most stabilized interaction. This study provides deeper insights on ZH as prominent source of bioactive compounds with potent antioxidant and anticancer effects. |
| first_indexed | 2024-03-09T11:57:56Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T11:57:56Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-c9eefc22875e491f930c12a966ac12de2023-11-30T23:07:04ZengMDPI AGLife2075-17292022-12-011313510.3390/life13010035Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational InvestigationNouha Bouali0Walid Sabri Hamadou1Riadh Badraoui2Ramzi Hadj Lajimi3Assia Hamdi4Mousa Alreshidi5Mohd Adnan6Zohra Soua7Arif Jamal Siddiqui8Emira Noumi9Mejdi Snoussi10Department of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaLaboratory of Galenic and Pharmacological Chemical Development of Drugs, University of Pharmacy, Monastir 5000, TunisiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaResearch Unit: Molecular Biology of Leukemia and Lymphoma, Department of Biochemistry, University of Medecine of Sousse, Sousse 4002, TunisiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, P.O. Box 2440, Hail 2440, Saudi ArabiaCancer is one of the major causes of death worldwide. The repercussions of conventional therapeutic approaches present a challenge in the delivery of new effective treatments. Thus, more attention is being awarded to natural products, mainly honey. Honey could be the basis for the development of new therapies for cancer patients. The aim of this study is to assess the phytochemical profiling, antioxidant, drug-likeness properties, and anticancer activity of <i>Ziziphus</i> honey (ZH) derived from the Hail region of Saudi Arabia. The phytochemical profiling using high resolution-liquid chromatography mass spectrometry (HR-LCMS) revealed 10 compounds belonging to several familial classes and one tripeptide. Potential antioxidant activity was noted as assessed by DPPH (IC<sub>50</sub> 0.670 mg/mL), ABTS (IC<sub>50</sub> 3.554 mg/mL), and β-carotene (IC<sub>50</sub> > 5 mg/mL). The ZH exerted a notable cytotoxic effect in a dose-dependent manner against three cancer cell lines: lung (A549), breast (MCF-7), and colon (HCT-116), with respective IC<sub>50</sub> values of 5.203%, 6.02%, and 7.257%. The drug-likeness investigation unveiled that most of the identified compounds meet Lipinski’s rule. The molecular docking analysis revealed interesting antioxidant and anticancer activities for most targeted proteins and supported the in vitro findings. The Miraxanthin-III compound exhibited the most stabilized interaction. This study provides deeper insights on ZH as prominent source of bioactive compounds with potent antioxidant and anticancer effects.https://www.mdpi.com/2075-1729/13/1/35anticancerantioxidantcomputational analysisHR-LCMS<i>Ziziphus</i> honey |
| spellingShingle | Nouha Bouali Walid Sabri Hamadou Riadh Badraoui Ramzi Hadj Lajimi Assia Hamdi Mousa Alreshidi Mohd Adnan Zohra Soua Arif Jamal Siddiqui Emira Noumi Mejdi Snoussi Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation Life anticancer antioxidant computational analysis HR-LCMS <i>Ziziphus</i> honey |
| title | Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation |
| title_full | Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation |
| title_fullStr | Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation |
| title_full_unstemmed | Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation |
| title_short | Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation |
| title_sort | phytochemical composition antioxidant and anticancer activities of sidr honey in vitro and in silico computational investigation |
| topic | anticancer antioxidant computational analysis HR-LCMS <i>Ziziphus</i> honey |
| url | https://www.mdpi.com/2075-1729/13/1/35 |
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