Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro
<em><strong>Objective(s):</strong></em> Malaria is an important parasitic disease with high morbidity and mortality in tropical areas. Resistance to most antimalarial drugs has encouraged the development of new drugs including natural products. Venom is a complex mixture of a...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_14975_e876d7961d833735d8ea2820a785a2f3.pdf |
| _version_ | 1811234973057810432 |
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| author | Fatemeh Hajialaiani Taher Elmi Maryam Mohammadi Delavr Shahbazzadeh Fatemeh Ghafarifar Abolhossein Dalimi Asl Mohammad Arjmand Fatemeh Tabatabaie Zahra Zamani |
| author_facet | Fatemeh Hajialaiani Taher Elmi Maryam Mohammadi Delavr Shahbazzadeh Fatemeh Ghafarifar Abolhossein Dalimi Asl Mohammad Arjmand Fatemeh Tabatabaie Zahra Zamani |
| author_sort | Fatemeh Hajialaiani |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> Malaria is an important parasitic disease with high morbidity and mortality in tropical areas. Resistance to most antimalarial drugs has encouraged the development of new drugs including natural products. Venom is a complex mixture of active pharmaceutical ingredients. The purpose of this study was to investigate the antimalarial activity of purified fractions of Naja naja oxiana. <br /><em><strong>Materials and Methods:</strong></em> Lyophilized venom was purified with a Sephacryl S-200 HR column and the fractions lyophilized and inhibitory concentration 50% against Plasmodium falciparum 3D7 in vitro obtained. The 4th fraction was run on a Mono Q column, and activity against P. falciparum was detected by lactate dehydrogenase assay and purity by SDS PAGE. Large scale culture of the parasite was carried out with and without the active fraction on the ring stage for 48 hr. The parasites were collected and lyophilized and analyzed by 1HNMR. Chemometrics studies were performed using MATLAB, differentiating metabolites were identified by Human Metabolic Database, and metabolic pathways by the Metaboanalyst online package. <br /><em><strong>Results:</strong></em> The active fraction from the ion exchange column had a 50% inhibitory concentration of 0.026 µg/ml on P. falciparum in vitro (P<0.001) with molecular weight of 63 kDa by SDS-PAGE and no hemolytic activity. Metabolomics studies on the two groups with and without the fraction identified 5 differentiating metabolites and a number of related pathways.<br /><em><strong>Conclusion:</strong></em> The metabolites were succinic acid, l-glutamic acid, pyruvic acid, cholesterol, and NAD. The changes in the Krebs cycle and metabolism pathways of nicotinamide and pyruvate were noticeable. |
| first_indexed | 2024-04-12T11:43:16Z |
| format | Article |
| id | doaj.art-c9ef4a8f5bbb4be08e2fcda2fb59f78e |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T11:43:16Z |
| publishDate | 2020-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c9ef4a8f5bbb4be08e2fcda2fb59f78e2022-12-22T03:34:30ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-04-0123453454310.22038/ijbms.2020.39386.934414975Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitroFatemeh Hajialaiani0Taher Elmi1Maryam Mohammadi2Delavr Shahbazzadeh3Fatemeh Ghafarifar4Abolhossein Dalimi Asl5Mohammad Arjmand6Fatemeh Tabatabaie7Zahra Zamani8Medical Parasitology Department, School of Medicine -International Campus, Iran University of Medical Sciences, Tehran, IranParasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranBiochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. IranLaboratory of Venom and Bio Therapeutics Molecules, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, IranBiochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. IranParasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranBiochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran<em><strong>Objective(s):</strong></em> Malaria is an important parasitic disease with high morbidity and mortality in tropical areas. Resistance to most antimalarial drugs has encouraged the development of new drugs including natural products. Venom is a complex mixture of active pharmaceutical ingredients. The purpose of this study was to investigate the antimalarial activity of purified fractions of Naja naja oxiana. <br /><em><strong>Materials and Methods:</strong></em> Lyophilized venom was purified with a Sephacryl S-200 HR column and the fractions lyophilized and inhibitory concentration 50% against Plasmodium falciparum 3D7 in vitro obtained. The 4th fraction was run on a Mono Q column, and activity against P. falciparum was detected by lactate dehydrogenase assay and purity by SDS PAGE. Large scale culture of the parasite was carried out with and without the active fraction on the ring stage for 48 hr. The parasites were collected and lyophilized and analyzed by 1HNMR. Chemometrics studies were performed using MATLAB, differentiating metabolites were identified by Human Metabolic Database, and metabolic pathways by the Metaboanalyst online package. <br /><em><strong>Results:</strong></em> The active fraction from the ion exchange column had a 50% inhibitory concentration of 0.026 µg/ml on P. falciparum in vitro (P<0.001) with molecular weight of 63 kDa by SDS-PAGE and no hemolytic activity. Metabolomics studies on the two groups with and without the fraction identified 5 differentiating metabolites and a number of related pathways.<br /><em><strong>Conclusion:</strong></em> The metabolites were succinic acid, l-glutamic acid, pyruvic acid, cholesterol, and NAD. The changes in the Krebs cycle and metabolism pathways of nicotinamide and pyruvate were noticeable.http://ijbms.mums.ac.ir/article_14975_e876d7961d833735d8ea2820a785a2f3.pdfchemometrics1hnmrmetabolomicsnaja naja oxiana venomplasmodium falciparum |
| spellingShingle | Fatemeh Hajialaiani Taher Elmi Maryam Mohammadi Delavr Shahbazzadeh Fatemeh Ghafarifar Abolhossein Dalimi Asl Mohammad Arjmand Fatemeh Tabatabaie Zahra Zamani Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro Iranian Journal of Basic Medical Sciences chemometrics 1hnmr metabolomics naja naja oxiana venom plasmodium falciparum |
| title | Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro |
| title_full | Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro |
| title_fullStr | Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro |
| title_full_unstemmed | Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro |
| title_short | Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro |
| title_sort | analysis of the active fraction of iranian naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1hnmr in vitro |
| topic | chemometrics 1hnmr metabolomics naja naja oxiana venom plasmodium falciparum |
| url | http://ijbms.mums.ac.ir/article_14975_e876d7961d833735d8ea2820a785a2f3.pdf |
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