PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines
Platelet-rich fibrin (PRF) is an autologous fibrin-rich matrix where activated platelets and leucocytes accumulate. PRF has a wide spectrum of clinical indications with the overall aim of supporting tissue regeneration which in dentistry includes the healing of healthy oral mucosa with epithelial ce...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Dentistry Journal |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6767/11/10/242 |
| _version_ | 1797574163504824320 |
|---|---|
| author | Layla Panahipour Rebecca Croci Sara Guarnieri Reinhard Gruber |
| author_facet | Layla Panahipour Rebecca Croci Sara Guarnieri Reinhard Gruber |
| author_sort | Layla Panahipour |
| collection | DOAJ |
| description | Platelet-rich fibrin (PRF) is an autologous fibrin-rich matrix where activated platelets and leucocytes accumulate. PRF has a wide spectrum of clinical indications with the overall aim of supporting tissue regeneration which in dentistry includes the healing of healthy oral mucosa with epithelial cells. In oral squamous cell carcinoma lesions, however, epithelial cells undergo malignant transformation, indicated by their unrestricted proliferation and migration potential, which should not be further enhanced by a wound-healing formula. Yet, little is known about how oral squamous cell carcinomas respond to PRF lysates. The aim of the present study was, therefore, to test the capacity of PRF lysates to change the transcriptome of HSC2 oral squamous carcinoma cells and perform bioassays to support the findings. Based on the RNAseq analysis, PRF lysates caused an increase in the genes functionally linked to cell replication and migration. In support of this screening approach, PRF lysates enhanced the proliferation of HSC2 oral squamous carcinoma cells, as indicated by <sup>3</sup>[H]-thymidine incorporation, cell counting, and the expression of proliferation-related genes. Moreover, PRF lysates sped up cell migration in a scratch assay requiring actin polymerization. Taken together, our data showing that PRF lysates are mitogenic and stimulate motility of oral squamous carcinoma cell lines could be an indication that treatment with PRF in cases of oral carcinoma should be carefully considered. |
| first_indexed | 2024-03-10T21:20:03Z |
| format | Article |
| id | doaj.art-c9ef9ce596834c8698aa2ab623f13d03 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-03-10T21:20:03Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-c9ef9ce596834c8698aa2ab623f13d032023-11-19T16:11:49ZengMDPI AGDentistry Journal2304-67672023-10-01111024210.3390/dj11100242PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell LinesLayla Panahipour0Rebecca Croci1Sara Guarnieri2Reinhard Gruber3Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaPlatelet-rich fibrin (PRF) is an autologous fibrin-rich matrix where activated platelets and leucocytes accumulate. PRF has a wide spectrum of clinical indications with the overall aim of supporting tissue regeneration which in dentistry includes the healing of healthy oral mucosa with epithelial cells. In oral squamous cell carcinoma lesions, however, epithelial cells undergo malignant transformation, indicated by their unrestricted proliferation and migration potential, which should not be further enhanced by a wound-healing formula. Yet, little is known about how oral squamous cell carcinomas respond to PRF lysates. The aim of the present study was, therefore, to test the capacity of PRF lysates to change the transcriptome of HSC2 oral squamous carcinoma cells and perform bioassays to support the findings. Based on the RNAseq analysis, PRF lysates caused an increase in the genes functionally linked to cell replication and migration. In support of this screening approach, PRF lysates enhanced the proliferation of HSC2 oral squamous carcinoma cells, as indicated by <sup>3</sup>[H]-thymidine incorporation, cell counting, and the expression of proliferation-related genes. Moreover, PRF lysates sped up cell migration in a scratch assay requiring actin polymerization. Taken together, our data showing that PRF lysates are mitogenic and stimulate motility of oral squamous carcinoma cell lines could be an indication that treatment with PRF in cases of oral carcinoma should be carefully considered.https://www.mdpi.com/2304-6767/11/10/242platelet-rich fibrinoral epithelial cellsoral squamous carcinoma cellsproliferationmigration |
| spellingShingle | Layla Panahipour Rebecca Croci Sara Guarnieri Reinhard Gruber PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines Dentistry Journal platelet-rich fibrin oral epithelial cells oral squamous carcinoma cells proliferation migration |
| title | PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines |
| title_full | PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines |
| title_fullStr | PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines |
| title_full_unstemmed | PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines |
| title_short | PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines |
| title_sort | prf lysates enhance the proliferation and migration of oral squamous carcinoma cell lines |
| topic | platelet-rich fibrin oral epithelial cells oral squamous carcinoma cells proliferation migration |
| url | https://www.mdpi.com/2304-6767/11/10/242 |
| work_keys_str_mv | AT laylapanahipour prflysatesenhancetheproliferationandmigrationoforalsquamouscarcinomacelllines AT rebeccacroci prflysatesenhancetheproliferationandmigrationoforalsquamouscarcinomacelllines AT saraguarnieri prflysatesenhancetheproliferationandmigrationoforalsquamouscarcinomacelllines AT reinhardgruber prflysatesenhancetheproliferationandmigrationoforalsquamouscarcinomacelllines |