Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/7/750 |
| _version_ | 1797529313834172416 |
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| author | Pasquale Marrazzo Valeria Pizzuti Silvia Zia Azzurra Sargenti Daniele Gazzola Barbara Roda Laura Bonsi Francesco Alviano |
| author_facet | Pasquale Marrazzo Valeria Pizzuti Silvia Zia Azzurra Sargenti Daniele Gazzola Barbara Roda Laura Bonsi Francesco Alviano |
| author_sort | Pasquale Marrazzo |
| collection | DOAJ |
| description | Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms. |
| first_indexed | 2024-03-10T10:12:03Z |
| format | Article |
| id | doaj.art-c9f384feac4947508068c342780b570f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:12:03Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c9f384feac4947508068c342780b570f2023-11-22T01:07:01ZengMDPI AGAntibiotics2079-63822021-06-0110775010.3390/antibiotics10070750Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial SecretomePasquale Marrazzo0Valeria Pizzuti1Silvia Zia2Azzurra Sargenti3Daniele Gazzola4Barbara Roda5Laura Bonsi6Francesco Alviano7Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40126 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40126 Bologna, ItalyStem Sel S.r.l., 40127 Bologna, ItalyCell Dynamics i.S.r.l., 40129 Bologna, ItalyCell Dynamics i.S.r.l., 40129 Bologna, ItalyStem Sel S.r.l., 40127 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40126 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40126 Bologna, ItalyAntibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.https://www.mdpi.com/2079-6382/10/7/750mesenchymal stem cellsstem cellsextracellular vesiclesexosomescell-therapyanti-inflammatory |
| spellingShingle | Pasquale Marrazzo Valeria Pizzuti Silvia Zia Azzurra Sargenti Daniele Gazzola Barbara Roda Laura Bonsi Francesco Alviano Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome Antibiotics mesenchymal stem cells stem cells extracellular vesicles exosomes cell-therapy anti-inflammatory |
| title | Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome |
| title_full | Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome |
| title_fullStr | Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome |
| title_full_unstemmed | Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome |
| title_short | Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome |
| title_sort | microfluidic tools for enhanced characterization of therapeutic stem cells and prediction of their potential antimicrobial secretome |
| topic | mesenchymal stem cells stem cells extracellular vesicles exosomes cell-therapy anti-inflammatory |
| url | https://www.mdpi.com/2079-6382/10/7/750 |
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