Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1
The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host <i>Sclerotinia sclerotiorum</i> strain DT-8. However, the mechanisms of...
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MDPI AG
2021-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/7/493 |
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| author | Zheng Qu Yanping Fu Yang Lin Zhenzhen Zhao Xuekun Zhang Jiasen Cheng Jiatao Xie Tao Chen Bo Li Daohong Jiang |
| author_facet | Zheng Qu Yanping Fu Yang Lin Zhenzhen Zhao Xuekun Zhang Jiasen Cheng Jiatao Xie Tao Chen Bo Li Daohong Jiang |
| author_sort | Zheng Qu |
| collection | DOAJ |
| description | The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host <i>Sclerotinia sclerotiorum</i> strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 <i>S. sclerotiorum</i> differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in <i>S. sclerotiorum</i> upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in <i>S. sclerotiorum</i>. |
| first_indexed | 2024-03-10T10:12:03Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T10:12:03Z |
| publishDate | 2021-06-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-c9f503a1433f4f858880dcdc3712a4072023-11-22T01:08:18ZengMDPI AGJournal of Fungi2309-608X2021-06-017749310.3390/jof7070493Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1Zheng Qu0Yanping Fu1Yang Lin2Zhenzhen Zhao3Xuekun Zhang4Jiasen Cheng5Jiatao Xie6Tao Chen7Bo Li8Daohong Jiang9State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaThe infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host <i>Sclerotinia sclerotiorum</i> strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 <i>S. sclerotiorum</i> differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in <i>S. sclerotiorum</i> upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in <i>S. sclerotiorum</i>.https://www.mdpi.com/2309-608X/7/7/493transcriptome<i>Sclerotinia sclerotiorum</i><i>Sclerotinia sclerotiorum</i> hypovirulence associated DNA virus 1mycoviruses |
| spellingShingle | Zheng Qu Yanping Fu Yang Lin Zhenzhen Zhao Xuekun Zhang Jiasen Cheng Jiatao Xie Tao Chen Bo Li Daohong Jiang Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 Journal of Fungi transcriptome <i>Sclerotinia sclerotiorum</i> <i>Sclerotinia sclerotiorum</i> hypovirulence associated DNA virus 1 mycoviruses |
| title | Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 |
| title_full | Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 |
| title_fullStr | Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 |
| title_full_unstemmed | Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 |
| title_short | Transcriptional Responses of <i>Sclerotinia sclerotiorum</i> to the Infection by SsHADV-1 |
| title_sort | transcriptional responses of i sclerotinia sclerotiorum i to the infection by sshadv 1 |
| topic | transcriptome <i>Sclerotinia sclerotiorum</i> <i>Sclerotinia sclerotiorum</i> hypovirulence associated DNA virus 1 mycoviruses |
| url | https://www.mdpi.com/2309-608X/7/7/493 |
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