Composition of the Oral Microbiome in Patients with Coronary Artery Disease
Introduction: Distinct microbial communities reside in the oral cavity and the composition of the oral microbiota has important implications for human health and disease. Identification of bacterial flora of the microbiome is done by metagenomic analysis of 16S ribosomal RNA sequences. Aim: The...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2020-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/14034/45246_CE[Ra1]_F(SHU)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| _version_ | 1818731064467652608 |
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| author | Thangam Menon Supraja Kalyanaraman Seethalakshmi Srinivasan |
| author_facet | Thangam Menon Supraja Kalyanaraman Seethalakshmi Srinivasan |
| author_sort | Thangam Menon |
| collection | DOAJ |
| description | Introduction: Distinct microbial communities reside in the oral
cavity and the composition of the oral microbiota has important
implications for human health and disease. Identification of
bacterial flora of the microbiome is done by metagenomic
analysis of 16S ribosomal RNA sequences.
Aim: The aim of this study was to characterise the human
microbiome in patients with Coronary Artery Disease (CAD) in
comparison with the normal human microbiome.
Materials and Methods: A pilot study was carried out in tertiary
hospital, Chennai. Oral mouthwash samples collected from
nine patients with CAD were selected, with one control group.
They were studied by metagenomic analysis of V3-V4 region of
16SrRNA gene sequences.. Sequencing of the variable V3 and
V4 regions was done using Illumina platform.
Results: The six major phyla, Firmicutes, Bacteroidetes,
Proteobacteria, Actinobacteria, Spirochaetes, and Fusobacteria
contained 99% of the taxa in all the samples analysed.
Conclusion: Diversity of the microbiome in patients with CAD
was similar to the normal human microbiome. |
| first_indexed | 2024-12-17T23:11:44Z |
| format | Article |
| id | doaj.art-c9f67b2582074bbf813114537fe2d4a9 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-17T23:11:44Z |
| publishDate | 2020-09-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-c9f67b2582074bbf813114537fe2d4a92022-12-21T21:29:06ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2020-09-01149DM01DM0310.7860/JCDR/2020/45246.14034Composition of the Oral Microbiome in Patients with Coronary Artery DiseaseThangam Menon0Supraja Kalyanaraman1Seethalakshmi Srinivasan2UGC BSR Faculty, Department of Microbiology, Dr ALMPGIBMS, University of Madras, Chennai, Tamil Nadu, India.Consultant Pulmonologist, Department of Respiratory Medicine, Madras Medical Mission, Chennai, Tamil Nadu, India.Research Fellow, Department of Microbiology, Dr ALMPGIBMS, University of Madras, Chennai, Tamil Nadu, India.Introduction: Distinct microbial communities reside in the oral cavity and the composition of the oral microbiota has important implications for human health and disease. Identification of bacterial flora of the microbiome is done by metagenomic analysis of 16S ribosomal RNA sequences. Aim: The aim of this study was to characterise the human microbiome in patients with Coronary Artery Disease (CAD) in comparison with the normal human microbiome. Materials and Methods: A pilot study was carried out in tertiary hospital, Chennai. Oral mouthwash samples collected from nine patients with CAD were selected, with one control group. They were studied by metagenomic analysis of V3-V4 region of 16SrRNA gene sequences.. Sequencing of the variable V3 and V4 regions was done using Illumina platform. Results: The six major phyla, Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Spirochaetes, and Fusobacteria contained 99% of the taxa in all the samples analysed. Conclusion: Diversity of the microbiome in patients with CAD was similar to the normal human microbiome.https://jcdr.net/articles/PDF/14034/45246_CE[Ra1]_F(SHU)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdfdysbiosismetagenomic analysisphylumtaxa |
| spellingShingle | Thangam Menon Supraja Kalyanaraman Seethalakshmi Srinivasan Composition of the Oral Microbiome in Patients with Coronary Artery Disease Journal of Clinical and Diagnostic Research dysbiosis metagenomic analysis phylum taxa |
| title | Composition of the Oral Microbiome in Patients with Coronary Artery Disease |
| title_full | Composition of the Oral Microbiome in Patients with Coronary Artery Disease |
| title_fullStr | Composition of the Oral Microbiome in Patients with Coronary Artery Disease |
| title_full_unstemmed | Composition of the Oral Microbiome in Patients with Coronary Artery Disease |
| title_short | Composition of the Oral Microbiome in Patients with Coronary Artery Disease |
| title_sort | composition of the oral microbiome in patients with coronary artery disease |
| topic | dysbiosis metagenomic analysis phylum taxa |
| url | https://jcdr.net/articles/PDF/14034/45246_CE[Ra1]_F(SHU)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| work_keys_str_mv | AT thangammenon compositionoftheoralmicrobiomeinpatientswithcoronaryarterydisease AT suprajakalyanaraman compositionoftheoralmicrobiomeinpatientswithcoronaryarterydisease AT seethalakshmisrinivasan compositionoftheoralmicrobiomeinpatientswithcoronaryarterydisease |