Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture
The carotenogenic thraustochytrid <i>Thraustochytrium</i> sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin,...
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MDPI AG
2022-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/7/416 |
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| author | Allison Leyton Carolina Shene Yusuf Chisti Juan A. Asenjo |
| author_facet | Allison Leyton Carolina Shene Yusuf Chisti Juan A. Asenjo |
| author_sort | Allison Leyton |
| collection | DOAJ |
| description | The carotenogenic thraustochytrid <i>Thraustochytrium</i> sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and β-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark; biomass concentration = 4.2 ± 1.1 g L<sup>−1</sup>) and 42.6% (glucose-based medium under light; biomass concentration = 3.3 ± 0.1 g L<sup>−1</sup>), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 ± 7 μg g<sup>−1</sup> dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 ± 4.3 mg g<sup>−1</sup> DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 ± 45 mg g<sup>−1</sup> DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly β-carotene. |
| first_indexed | 2024-03-09T13:29:35Z |
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| language | English |
| last_indexed | 2024-03-09T13:29:35Z |
| publishDate | 2022-06-01 |
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| series | Marine Drugs |
| spelling | doaj.art-c9f73ee6d0bb412e885e8ca55b0aab3d2023-11-30T21:20:02ZengMDPI AGMarine Drugs1660-33972022-06-0120741610.3390/md20070416Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch CultureAllison Leyton0Carolina Shene1Yusuf Chisti2Juan A. Asenjo3Centre for Biotechnology and Bioengineering (CeBiB), Center of Food Biotechnology and Bioseparations, BIOREN and Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar 01145, Temuco 4780000, ChileCentre for Biotechnology and Bioengineering (CeBiB), Center of Food Biotechnology and Bioseparations, BIOREN and Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar 01145, Temuco 4780000, ChileSchool of Engineering, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandCentre for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering and Biotechnology, Universidad de Chile, Beauchef 851, Santiago 8370459, ChileThe carotenogenic thraustochytrid <i>Thraustochytrium</i> sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and β-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark; biomass concentration = 4.2 ± 1.1 g L<sup>−1</sup>) and 42.6% (glucose-based medium under light; biomass concentration = 3.3 ± 0.1 g L<sup>−1</sup>), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 ± 7 μg g<sup>−1</sup> dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 ± 4.3 mg g<sup>−1</sup> DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 ± 45 mg g<sup>−1</sup> DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly β-carotene.https://www.mdpi.com/1660-3397/20/7/416<i>Thraustochytrium</i> sp.thraustochytridscarotenoidscanthaxanthindocosahexaenoic acidphospholipids |
| spellingShingle | Allison Leyton Carolina Shene Yusuf Chisti Juan A. Asenjo Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture Marine Drugs <i>Thraustochytrium</i> sp. thraustochytrids carotenoids canthaxanthin docosahexaenoic acid phospholipids |
| title | Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture |
| title_full | Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture |
| title_fullStr | Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture |
| title_full_unstemmed | Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture |
| title_short | Production of Carotenoids and Phospholipids by <i>Thraustochytrium</i> sp. in Batch and Repeated-Batch Culture |
| title_sort | production of carotenoids and phospholipids by i thraustochytrium i sp in batch and repeated batch culture |
| topic | <i>Thraustochytrium</i> sp. thraustochytrids carotenoids canthaxanthin docosahexaenoic acid phospholipids |
| url | https://www.mdpi.com/1660-3397/20/7/416 |
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