Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation
Sophora japonica L. is widely consumed in China because of its medicinal and nutritional value. Its quality is greatly affected by the accumulation of metabolites, which varies with the stage of flower development. However, changes in the characteristics of the secondary metabolites during flower ma...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.916410/full |
| _version_ | 1811221361493803008 |
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| author | Ji-Rui Wang Ji-Rui Wang Xu-Hong Song Xu-Hong Song Long-Yun Li Long-Yun Li Si-Jia Gao Fang-Hong Shang Xiao-Mei Zhang Yong Yang |
| author_facet | Ji-Rui Wang Ji-Rui Wang Xu-Hong Song Xu-Hong Song Long-Yun Li Long-Yun Li Si-Jia Gao Fang-Hong Shang Xiao-Mei Zhang Yong Yang |
| author_sort | Ji-Rui Wang |
| collection | DOAJ |
| description | Sophora japonica L. is widely consumed in China because of its medicinal and nutritional value. Its quality is greatly affected by the accumulation of metabolites, which varies with the stage of flower development. However, changes in the characteristics of the secondary metabolites during flower maturity remain unclear. Ultra-high-performance liquid chromatography coupled with electrospray ionization-triple quadrupole-linear ion trap mass spectrometry (UPLC–ESI–QTRAP–MS/MS) revealed dynamic changes in the secondary metabolites of S. japonica during the five flower-maturity stages. We monitored 331 metabolites and screened 164. The differential metabolites showed seven trends during flower maturation, with flavonoids and phenolic acids having the most varied expressions. Flower buds (S2–S3) are rich in flavonoids and are thus suitable for use in high-quality medicine or industrial extraction. Our study provides an empirical basis for the informed harvesting of S. japonica based on its mode of utilization. |
| first_indexed | 2024-04-12T07:58:05Z |
| format | Article |
| id | doaj.art-c9f79018000a4cc0ad103d0c2d1abf81 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T07:58:05Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c9f79018000a4cc0ad103d0c2d1abf812022-12-22T03:41:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.916410916410Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturationJi-Rui Wang0Ji-Rui Wang1Xu-Hong Song2Xu-Hong Song3Long-Yun Li4Long-Yun Li5Si-Jia Gao6Fang-Hong Shang7Xiao-Mei Zhang8Yong Yang9Three Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaChongqing Sub-Center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaChongqing Sub-Center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaChongqing Sub-Center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaThree Grade Laboratory of Chinese Medicine Chemistry, Chongqing Academy of Chinese Materia Medica, Chongqing, ChinaSophora japonica L. is widely consumed in China because of its medicinal and nutritional value. Its quality is greatly affected by the accumulation of metabolites, which varies with the stage of flower development. However, changes in the characteristics of the secondary metabolites during flower maturity remain unclear. Ultra-high-performance liquid chromatography coupled with electrospray ionization-triple quadrupole-linear ion trap mass spectrometry (UPLC–ESI–QTRAP–MS/MS) revealed dynamic changes in the secondary metabolites of S. japonica during the five flower-maturity stages. We monitored 331 metabolites and screened 164. The differential metabolites showed seven trends during flower maturation, with flavonoids and phenolic acids having the most varied expressions. Flower buds (S2–S3) are rich in flavonoids and are thus suitable for use in high-quality medicine or industrial extraction. Our study provides an empirical basis for the informed harvesting of S. japonica based on its mode of utilization.https://www.frontiersin.org/articles/10.3389/fpls.2022.916410/fullSophora japonica L.flower maturationdifferential metabolitesflavonoidsmultivariate analysis |
| spellingShingle | Ji-Rui Wang Ji-Rui Wang Xu-Hong Song Xu-Hong Song Long-Yun Li Long-Yun Li Si-Jia Gao Fang-Hong Shang Xiao-Mei Zhang Yong Yang Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation Frontiers in Plant Science Sophora japonica L. flower maturation differential metabolites flavonoids multivariate analysis |
| title | Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation |
| title_full | Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation |
| title_fullStr | Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation |
| title_full_unstemmed | Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation |
| title_short | Metabolomic analysis reveals dynamic changes in secondary metabolites of Sophora japonica L. during flower maturation |
| title_sort | metabolomic analysis reveals dynamic changes in secondary metabolites of sophora japonica l during flower maturation |
| topic | Sophora japonica L. flower maturation differential metabolites flavonoids multivariate analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.916410/full |
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