Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens

A feeding trial was conducted to evaluate the effects of Bacillus-based probiotics on growth performance, intestinal histo-morphology, gut microbial population and immune response in broilers. A total of 2000 Hubbard Classic day-old chicks were randomly enrolled in four experimental groups and 4 rep...

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Main Authors: Mohammad Arif, Md. Akteruzzaman, Tuhin-Al-Ferdous, Sk Shaheenur Islam, Bidhan Chandra Das, Mahbubul Pratik Siddique, S. M. Lutful Kabir
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Veterinary and Animal Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2451943X2100051X
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author Mohammad Arif
Md. Akteruzzaman
Tuhin-Al-Ferdous
Sk Shaheenur Islam
Bidhan Chandra Das
Mahbubul Pratik Siddique
S. M. Lutful Kabir
author_facet Mohammad Arif
Md. Akteruzzaman
Tuhin-Al-Ferdous
Sk Shaheenur Islam
Bidhan Chandra Das
Mahbubul Pratik Siddique
S. M. Lutful Kabir
author_sort Mohammad Arif
collection DOAJ
description A feeding trial was conducted to evaluate the effects of Bacillus-based probiotics on growth performance, intestinal histo-morphology, gut microbial population and immune response in broilers. A total of 2000 Hubbard Classic day-old chicks were randomly enrolled in four experimental groups and 4 replicates of 500 birds in each group, and reared for 35 days under a low- level of biosecurity measures. The trial groups were assigned treatment-1 (T1): basal diet(control), treatment-2 (T2): basal diet plus Bacillus licheniformis (DSM17236), treatment-3 (T3): basal diet plus Bacillus subtilis (PB6), and treatment-4 (T4) basal diet plus 4% Flavomycin. All four groups were fed with maize-soybean based prepared feeds (starter, grower and finisher). Dietary inclusion of B. licheniformis significantly improved body weight gain and lessened FCR in T2 compared to other groups (p < 0.05). Probiotics increased the population of Bacillus spp. and decreased the population of Clostrium perfringens, Salmonella spp. and Escherichia coli in the jejunum and ileum in broiler birds on day 21 and 35 (p < 0.05). The highest antibody production was observed in B. licheniformis treated group (T2) compared to other probiotic treated group (T1). Taken together, the study findings suggest that B. licheniformis probiotics could be used as a feasible alternative to antimicrobials in the broiler production considering beneficial impacts at low biosecurity broiler farms.
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spelling doaj.art-c9f8e4b06cc94eb38fed659d479260462022-12-21T18:43:36ZengElsevierVeterinary and Animal Science2451-943X2021-12-0114100216Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickensMohammad Arif0Md. Akteruzzaman1 Tuhin-Al-Ferdous2Sk Shaheenur Islam3Bidhan Chandra Das4Mahbubul Pratik Siddique5S. M. Lutful Kabir6Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Department of Livestock Services, Krishi Khamar Sarak, Farmgate, Dhaka 1215, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.; Corresponding author at: Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, BangladeshA feeding trial was conducted to evaluate the effects of Bacillus-based probiotics on growth performance, intestinal histo-morphology, gut microbial population and immune response in broilers. A total of 2000 Hubbard Classic day-old chicks were randomly enrolled in four experimental groups and 4 replicates of 500 birds in each group, and reared for 35 days under a low- level of biosecurity measures. The trial groups were assigned treatment-1 (T1): basal diet(control), treatment-2 (T2): basal diet plus Bacillus licheniformis (DSM17236), treatment-3 (T3): basal diet plus Bacillus subtilis (PB6), and treatment-4 (T4) basal diet plus 4% Flavomycin. All four groups were fed with maize-soybean based prepared feeds (starter, grower and finisher). Dietary inclusion of B. licheniformis significantly improved body weight gain and lessened FCR in T2 compared to other groups (p < 0.05). Probiotics increased the population of Bacillus spp. and decreased the population of Clostrium perfringens, Salmonella spp. and Escherichia coli in the jejunum and ileum in broiler birds on day 21 and 35 (p < 0.05). The highest antibody production was observed in B. licheniformis treated group (T2) compared to other probiotic treated group (T1). Taken together, the study findings suggest that B. licheniformis probiotics could be used as a feasible alternative to antimicrobials in the broiler production considering beneficial impacts at low biosecurity broiler farms.http://www.sciencedirect.com/science/article/pii/S2451943X2100051XBacillus licheniformisBacillus subtilisbroiler chickenprobioticgut histomorphologyintestinal microflora
spellingShingle Mohammad Arif
Md. Akteruzzaman
Tuhin-Al-Ferdous
Sk Shaheenur Islam
Bidhan Chandra Das
Mahbubul Pratik Siddique
S. M. Lutful Kabir
Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
Veterinary and Animal Science
Bacillus licheniformis
Bacillus subtilis
broiler chicken
probiotic
gut histomorphology
intestinal microflora
title Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
title_full Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
title_fullStr Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
title_full_unstemmed Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
title_short Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens
title_sort dietary supplementation of bacillus based probiotics on the growth performance gut morphology intestinal microbiota and immune response in low biosecurity broiler chickens
topic Bacillus licheniformis
Bacillus subtilis
broiler chicken
probiotic
gut histomorphology
intestinal microflora
url http://www.sciencedirect.com/science/article/pii/S2451943X2100051X
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