An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR)
Abstract Background Baculoviruses are significant biocontrol agents of pests in agriculture; however, ultraviolet light B (UV-B) and high temperature inactivate them in the environment within a short time. Results In this study, formulations of Helicoverpa armigera nucleopolyhedrovirus Turkey isolat...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-022-00552-4 |
| _version_ | 1818211410339954688 |
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| author | Gozde Busra Eroglu Zihni Demırbag |
| author_facet | Gozde Busra Eroglu Zihni Demırbag |
| author_sort | Gozde Busra Eroglu |
| collection | DOAJ |
| description | Abstract Background Baculoviruses are significant biocontrol agents of pests in agriculture; however, ultraviolet light B (UV-B) and high temperature inactivate them in the environment within a short time. Results In this study, formulations of Helicoverpa armigera nucleopolyhedrovirus Turkey isolate (HearNPV-TR) were prepared. Crude virus, the oil-based formulations of the virus, and a commercial H. armigera NPV product were exposed to UV-B light and various temperatures (28, 35, and 42 °C) at different periods (0, 1, 3, 5 h.). While the UV-B application decreased the virulence of crude virus up to 32% after 5 h. exposure, it decreased the virulence of oil-based formulation by (15%). Similarly, the virulence of the crude virus by 70% at 42 °C, while was reduced by 10% in the formulations at the same temperature. Therefore, 5% oil formulation was enough to keep the virulence of the virus underexposed to UV-B and high temperatures. LC95 values were calculated as 1 × 108 OBs/ml−1 in concentration–response tests, and mortality rates were 90 and 92% in pot assays for commercial product and 5% oil formulation, respectively. Conclusions The results showed that 5% oil formulation of HearNPV-TR was an important biocontrol preparation to protect virus virulence under suppressive environmental conditions. |
| first_indexed | 2024-12-12T05:32:03Z |
| format | Article |
| id | doaj.art-c9f8ea2a15b1431388cc54a612f81348 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-12T05:32:03Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-c9f8ea2a15b1431388cc54a612f813482022-12-22T00:36:17ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-05-013211710.1186/s41938-022-00552-4An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR)Gozde Busra Eroglu0Zihni Demırbag1Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical UniversityDepartment of Biology, Faculty of Science, Karadeniz Technical UniversityAbstract Background Baculoviruses are significant biocontrol agents of pests in agriculture; however, ultraviolet light B (UV-B) and high temperature inactivate them in the environment within a short time. Results In this study, formulations of Helicoverpa armigera nucleopolyhedrovirus Turkey isolate (HearNPV-TR) were prepared. Crude virus, the oil-based formulations of the virus, and a commercial H. armigera NPV product were exposed to UV-B light and various temperatures (28, 35, and 42 °C) at different periods (0, 1, 3, 5 h.). While the UV-B application decreased the virulence of crude virus up to 32% after 5 h. exposure, it decreased the virulence of oil-based formulation by (15%). Similarly, the virulence of the crude virus by 70% at 42 °C, while was reduced by 10% in the formulations at the same temperature. Therefore, 5% oil formulation was enough to keep the virulence of the virus underexposed to UV-B and high temperatures. LC95 values were calculated as 1 × 108 OBs/ml−1 in concentration–response tests, and mortality rates were 90 and 92% in pot assays for commercial product and 5% oil formulation, respectively. Conclusions The results showed that 5% oil formulation of HearNPV-TR was an important biocontrol preparation to protect virus virulence under suppressive environmental conditions.https://doi.org/10.1186/s41938-022-00552-4BaculovirusHelicoverpa armigeraMicrobial controlOil-based formulation |
| spellingShingle | Gozde Busra Eroglu Zihni Demırbag An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) Egyptian Journal of Biological Pest Control Baculovirus Helicoverpa armigera Microbial control Oil-based formulation |
| title | An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) |
| title_full | An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) |
| title_fullStr | An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) |
| title_full_unstemmed | An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) |
| title_short | An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) |
| title_sort | environmentally safe and tolerant microbial insecticide utilizing helicoverpa armigera single nucleopolyhedrovirus hearnpv tr |
| topic | Baculovirus Helicoverpa armigera Microbial control Oil-based formulation |
| url | https://doi.org/10.1186/s41938-022-00552-4 |
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