Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection
A modular, multi-purpose, and cost-effective electrochemical biosensor based on a five-stranded four-way junction (5S-4WJ) system was developed for SARS-CoV-2 (genes S and N) and Influenza A virus (gene M) detection. The 5S-4WJ structure consists of an electrode-immobilized universal stem-loop (USL)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/9/874 |
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| author | Andrew Murray Julio Ojeda Omar El Merhebi Percy Calvo-Marzal Yulia Gerasimova Karin Chumbimuni-Torres |
| author_facet | Andrew Murray Julio Ojeda Omar El Merhebi Percy Calvo-Marzal Yulia Gerasimova Karin Chumbimuni-Torres |
| author_sort | Andrew Murray |
| collection | DOAJ |
| description | A modular, multi-purpose, and cost-effective electrochemical biosensor based on a five-stranded four-way junction (5S-4WJ) system was developed for SARS-CoV-2 (genes S and N) and Influenza A virus (gene M) detection. The 5S-4WJ structure consists of an electrode-immobilized universal stem-loop (USL) strand, two auxiliary DNA strands, and a universal methylene blue redox strand (UMeB). This design allows for the detection of specific nucleic acid sequences using square wave voltammetry (SWV). The sequence-specific auxiliary DNA strands (m and f) ensure selectivity of the biosensor for target recognition utilizing the same USL and UMeB components. An important feature of this biosensor is the ability to reuse the USL-modified electrodes to detect the same or alternative targets in new samples. This is accomplished by a simple procedure involving rinsing the electrodes with water to disrupt the 5S-4WJ structure and subsequent re-hybridization of the USL strand with the appropriate set of strands for a new analysis. The biosensor exhibited minimal loss in signal after rehybridization, demonstrating its potential as a viable multiplex assay for both current and future pathogens, with a low limit of quantification (LOQ) of as low as 17 pM. |
| first_indexed | 2024-03-10T22:59:41Z |
| format | Article |
| id | doaj.art-c9f9f30cee514bb89777db66b49abfe4 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T22:59:41Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-c9f9f30cee514bb89777db66b49abfe42023-11-19T09:47:32ZengMDPI AGBiosensors2079-63742023-09-0113987410.3390/bios13090874Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A DetectionAndrew Murray0Julio Ojeda1Omar El Merhebi2Percy Calvo-Marzal3Yulia Gerasimova4Karin Chumbimuni-Torres5Department of Chemistry, University of Central Florida, Orlando, FL 32816, USADepartment of Chemistry, University of Central Florida, Orlando, FL 32816, USADepartment of Chemistry, University of Central Florida, Orlando, FL 32816, USADepartment of Chemistry, University of Central Florida, Orlando, FL 32816, USADepartment of Chemistry, University of Central Florida, Orlando, FL 32816, USADepartment of Chemistry, University of Central Florida, Orlando, FL 32816, USAA modular, multi-purpose, and cost-effective electrochemical biosensor based on a five-stranded four-way junction (5S-4WJ) system was developed for SARS-CoV-2 (genes S and N) and Influenza A virus (gene M) detection. The 5S-4WJ structure consists of an electrode-immobilized universal stem-loop (USL) strand, two auxiliary DNA strands, and a universal methylene blue redox strand (UMeB). This design allows for the detection of specific nucleic acid sequences using square wave voltammetry (SWV). The sequence-specific auxiliary DNA strands (m and f) ensure selectivity of the biosensor for target recognition utilizing the same USL and UMeB components. An important feature of this biosensor is the ability to reuse the USL-modified electrodes to detect the same or alternative targets in new samples. This is accomplished by a simple procedure involving rinsing the electrodes with water to disrupt the 5S-4WJ structure and subsequent re-hybridization of the USL strand with the appropriate set of strands for a new analysis. The biosensor exhibited minimal loss in signal after rehybridization, demonstrating its potential as a viable multiplex assay for both current and future pathogens, with a low limit of quantification (LOQ) of as low as 17 pM.https://www.mdpi.com/2079-6374/13/9/874four-way junctionSARS-CoV-2Influenza Abiosensormicroliter detection |
| spellingShingle | Andrew Murray Julio Ojeda Omar El Merhebi Percy Calvo-Marzal Yulia Gerasimova Karin Chumbimuni-Torres Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection Biosensors four-way junction SARS-CoV-2 Influenza A biosensor microliter detection |
| title | Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection |
| title_full | Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection |
| title_fullStr | Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection |
| title_full_unstemmed | Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection |
| title_short | Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection |
| title_sort | cost effective modular biosensor for sars cov 2 and influenza a detection |
| topic | four-way junction SARS-CoV-2 Influenza A biosensor microliter detection |
| url | https://www.mdpi.com/2079-6374/13/9/874 |
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