<i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS
<i>Diplopterys pubipetala</i> (Malpighiaceae) is a liana native to the Brazilian Cerrado biome, traditionally used in Ayahuasca preparations. Despite its cultural importance, research on its chemical composition and biological activities, which may have therapeutic potential, is limited....
| প্রধান লেখক: | , , , , , , , , , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
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MDPI AG
2025-02-01
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| মালা: | Molecules |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://www.mdpi.com/1420-3049/30/4/946 |
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| author | Veronica de Melo Sacramento Vanessa de Andrade Royo Pedro Henrique Fonseca Veloso Kamila Soares Freitas Souto Alisson Samuel Portes Caldeira Carlos Henrique Gomes Martins Sara Lemes de Souza Ezequias Pessoa de Siqueira Fernando Ribeiro Cassiano Afrânio Farias de Melo Júnior Dario Alves de Oliveira Elytania Veiga Mnezes Tânia Maria de Almeida Alves |
| author_facet | Veronica de Melo Sacramento Vanessa de Andrade Royo Pedro Henrique Fonseca Veloso Kamila Soares Freitas Souto Alisson Samuel Portes Caldeira Carlos Henrique Gomes Martins Sara Lemes de Souza Ezequias Pessoa de Siqueira Fernando Ribeiro Cassiano Afrânio Farias de Melo Júnior Dario Alves de Oliveira Elytania Veiga Mnezes Tânia Maria de Almeida Alves |
| author_sort | Veronica de Melo Sacramento |
| collection | DOAJ |
| description | <i>Diplopterys pubipetala</i> (Malpighiaceae) is a liana native to the Brazilian Cerrado biome, traditionally used in Ayahuasca preparations. Despite its cultural importance, research on its chemical composition and biological activities, which may have therapeutic potential, is limited. This study investigated the volatile and non-volatile secondary metabolites of <i>D. pubipetala</i> leaves, their antioxidant capacity, and their antibacterial and antifungal activities. Volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS) coupled to solid-phase microextraction (SPME), while non-volatile compounds were annotated using UHPLC-MS/MS-ESI-Q-TOF. Antioxidant capacity was evaluated by DPPH assay, and antimicrobial activity was assessed in vitro against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Candida</i> species (<i>C. albicans</i>, <i>C. tropicalis</i>, <i>C. glabrata</i>). GC-MS analysis revealed 25 predominant volatile compounds, including ethyl dodecanoate, ethyl tetradecanoate, nonanoic acid, and 5-methylhexan-2-one, with documented antifungal, antioxidant, and antimicrobial activities. The crude extract and ethyl acetate fraction showed strong antioxidant capacity (EC<sub>50</sub> 9.83 µg/mL and 6.42 µg/mL, respectively), and antifungal effects were observed against <i>Candida</i> species. This study provides the first comprehensive investigation of the antioxidant capacity and antibacterial and antifungal activities of <i>D. pubipetala</i>, together with a detailed chemical profile of its volatile compounds. |
| first_indexed | 2025-03-14T14:56:24Z |
| format | Article |
| id | doaj.art-c9fcf695e6fe4cd39fc0b51cbb05b6bb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2025-03-14T14:56:24Z |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c9fcf695e6fe4cd39fc0b51cbb05b6bb2025-02-25T13:41:04ZengMDPI AGMolecules1420-30492025-02-0130494610.3390/molecules30040946<i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MSVeronica de Melo Sacramento0Vanessa de Andrade Royo1Pedro Henrique Fonseca Veloso2Kamila Soares Freitas Souto3Alisson Samuel Portes Caldeira4Carlos Henrique Gomes Martins5Sara Lemes de Souza6Ezequias Pessoa de Siqueira7Fernando Ribeiro Cassiano8Afrânio Farias de Melo Júnior9Dario Alves de Oliveira10Elytania Veiga Mnezes11Tânia Maria de Almeida Alves12Natural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilNatural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilNatural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilNatural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilLaboratory of Bioactive Natural Products Chemistry, René Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, BrazilAntimicrobial Testing Laboratory, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-320, MG, BrazilAntimicrobial Testing Laboratory, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-320, MG, BrazilLaboratory of Bioactive Natural Products Chemistry, René Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, BrazilDepartment of Exact Sciences, Center for Exact and Technological Sciences, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilBioprospecting and Genetic Resources Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilBioprospecting and Genetic Resources Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilBioprospecting and Genetic Resources Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, BrazilLaboratory of Bioactive Natural Products Chemistry, René Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, Brazil<i>Diplopterys pubipetala</i> (Malpighiaceae) is a liana native to the Brazilian Cerrado biome, traditionally used in Ayahuasca preparations. Despite its cultural importance, research on its chemical composition and biological activities, which may have therapeutic potential, is limited. This study investigated the volatile and non-volatile secondary metabolites of <i>D. pubipetala</i> leaves, their antioxidant capacity, and their antibacterial and antifungal activities. Volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS) coupled to solid-phase microextraction (SPME), while non-volatile compounds were annotated using UHPLC-MS/MS-ESI-Q-TOF. Antioxidant capacity was evaluated by DPPH assay, and antimicrobial activity was assessed in vitro against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Candida</i> species (<i>C. albicans</i>, <i>C. tropicalis</i>, <i>C. glabrata</i>). GC-MS analysis revealed 25 predominant volatile compounds, including ethyl dodecanoate, ethyl tetradecanoate, nonanoic acid, and 5-methylhexan-2-one, with documented antifungal, antioxidant, and antimicrobial activities. The crude extract and ethyl acetate fraction showed strong antioxidant capacity (EC<sub>50</sub> 9.83 µg/mL and 6.42 µg/mL, respectively), and antifungal effects were observed against <i>Candida</i> species. This study provides the first comprehensive investigation of the antioxidant capacity and antibacterial and antifungal activities of <i>D. pubipetala</i>, together with a detailed chemical profile of its volatile compounds.https://www.mdpi.com/1420-3049/30/4/946biological propertiesnatural productsbioactive compoundsantimicrobial activitytherapeutic potential |
| spellingShingle | Veronica de Melo Sacramento Vanessa de Andrade Royo Pedro Henrique Fonseca Veloso Kamila Soares Freitas Souto Alisson Samuel Portes Caldeira Carlos Henrique Gomes Martins Sara Lemes de Souza Ezequias Pessoa de Siqueira Fernando Ribeiro Cassiano Afrânio Farias de Melo Júnior Dario Alves de Oliveira Elytania Veiga Mnezes Tânia Maria de Almeida Alves <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS Molecules biological properties natural products bioactive compounds antimicrobial activity therapeutic potential |
| title | <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS |
| title_full | <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS |
| title_fullStr | <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS |
| title_full_unstemmed | <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS |
| title_short | <i>Diplopterys pubipetala</i> (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS/MS and GC/MS |
| title_sort | i diplopterys pubipetala i malpighiaceae insights into antioxidant antibacterial and antifungal activities with chemical composition analysis via uhplc ms ms and gc ms |
| topic | biological properties natural products bioactive compounds antimicrobial activity therapeutic potential |
| url | https://www.mdpi.com/1420-3049/30/4/946 |
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