Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2
Introduction The rapid surge of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) cases globally makes it essential for rapid diagnosis of coronavirus disease 2019 (COVID-19). Real-time reverse-transcription polymerase chain reaction (rtRT-PCR) remains as the gold standard to detect COVID...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2022-09-01
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| Series: | Journal of Laboratory Physicians |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1744237 |
| _version_ | 1818049789781082112 |
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| author | Alice P. Selvabai R Lino V. Koshy Priyadarshini Shanmugam |
| author_facet | Alice P. Selvabai R Lino V. Koshy Priyadarshini Shanmugam |
| author_sort | Alice P. Selvabai R |
| collection | DOAJ |
| description | Introduction The rapid surge of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) cases globally makes it essential for rapid diagnosis of coronavirus disease 2019 (COVID-19). Real-time reverse-transcription polymerase chain reaction (rtRT-PCR) remains as the gold standard to detect COVID-19 cases because of its greater sensitivity and specificity. However, because of its prolonged turnaround time and technical expertise, recommendations have been made to employ the use of rapid diagnostic test for rapid diagnosis and to curb the spread of the disease.
Methods This prospective study was performed in a tertiary COVID-19 care hospital located amidst the semi-urban settings. Both nasopharyngeal and throat swabs collected from the COVID 19 suspected study participants were subjected to both COVID 19 rtRT-PCR and rapid antigen testing.
Results Of the total 599 samples tested by rtRT-PCR, 310 (52%) were positive and 289 (48%) tested negative for SARS-CoV-2. Of the 599 samples tested by rapid antigen test (RAT), 230 (38%) were positive and 369 (62%) were negative. The overall sensitivity and specificity of our study kit was found to be 74.19 and 100%, respectively. The sensitivity of the RAT greatly overlaps with the viral load which is determined by the cycle threshold (CT) values of SARS-CoV-2, E gene, and RdRp gene.
Conclusion RAT yields rapid results within a short-turnaround time and found to be cost effective. Therefore, this test can be adopted in areas with rapid surge in SARS-CoV-2 cases which can help to rapidly identify the positive cases and to implement isolation and infection control measures. |
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| format | Article |
| id | doaj.art-c9fe18cdb495448a9b8bd5d587342a6c |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-10T10:43:10Z |
| publishDate | 2022-09-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-c9fe18cdb495448a9b8bd5d587342a6c2022-12-22T01:52:15ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262022-09-01140332432810.1055/s-0042-1744237Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2Alice P. Selvabai R0Lino V. Koshy1Priyadarshini Shanmugam2Department of Microbiology, Chettinad Hospital and Research Institute, Kelambakkam, Tamil Nadu, IndiaDepartment of Microbiology, Chettinad Hospital and Research Institute, Kelambakkam, Tamil Nadu, IndiaDepartment of Microbiology, Chettinad Hospital and Research Institute, Kelambakkam, Tamil Nadu, IndiaIntroduction The rapid surge of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) cases globally makes it essential for rapid diagnosis of coronavirus disease 2019 (COVID-19). Real-time reverse-transcription polymerase chain reaction (rtRT-PCR) remains as the gold standard to detect COVID-19 cases because of its greater sensitivity and specificity. However, because of its prolonged turnaround time and technical expertise, recommendations have been made to employ the use of rapid diagnostic test for rapid diagnosis and to curb the spread of the disease. Methods This prospective study was performed in a tertiary COVID-19 care hospital located amidst the semi-urban settings. Both nasopharyngeal and throat swabs collected from the COVID 19 suspected study participants were subjected to both COVID 19 rtRT-PCR and rapid antigen testing. Results Of the total 599 samples tested by rtRT-PCR, 310 (52%) were positive and 289 (48%) tested negative for SARS-CoV-2. Of the 599 samples tested by rapid antigen test (RAT), 230 (38%) were positive and 369 (62%) were negative. The overall sensitivity and specificity of our study kit was found to be 74.19 and 100%, respectively. The sensitivity of the RAT greatly overlaps with the viral load which is determined by the cycle threshold (CT) values of SARS-CoV-2, E gene, and RdRp gene. Conclusion RAT yields rapid results within a short-turnaround time and found to be cost effective. Therefore, this test can be adopted in areas with rapid surge in SARS-CoV-2 cases which can help to rapidly identify the positive cases and to implement isolation and infection control measures.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1744237novel corona virussars-cov-2rapid diagnostic testreal-time rt-pcrsensitivityspecificitypoint of care test |
| spellingShingle | Alice P. Selvabai R Lino V. Koshy Priyadarshini Shanmugam Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 Journal of Laboratory Physicians novel corona virus sars-cov-2 rapid diagnostic test real-time rt-pcr sensitivity specificity point of care test |
| title | Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 |
| title_full | Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 |
| title_fullStr | Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 |
| title_full_unstemmed | Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 |
| title_short | Diagnostic Efficacy of COVID-19 Rapid Antigen Detection Card in Diagnosis of SARS-CoV-2 |
| title_sort | diagnostic efficacy of covid 19 rapid antigen detection card in diagnosis of sars cov 2 |
| topic | novel corona virus sars-cov-2 rapid diagnostic test real-time rt-pcr sensitivity specificity point of care test |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1744237 |
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