Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia
Introduction: The secondary genetic changes other than the PML-RARA fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia (AML). However, the prognosti...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2010-09-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/253 |
| _version_ | 1797706081209679872 |
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| author | Marjan Yaghmaie Hossein Mozdarani Kamran Alimoghaddam Ardeshir Ghavamzadeh Seyed Hamiollah Ghaffari |
| author_facet | Marjan Yaghmaie Hossein Mozdarani Kamran Alimoghaddam Ardeshir Ghavamzadeh Seyed Hamiollah Ghaffari |
| author_sort | Marjan Yaghmaie |
| collection | DOAJ |
| description | Introduction: The secondary genetic changes other than the PML-RARA fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia (AML). However, the prognostic significance of FLT3 mutations in acute promyelocytic leukemia (APL) is not firmly established.
Patients & Methods: FLT3 ITD screening by fragment length analysis and FLT3 D835 mutation by melting curve analysis in 23 APL samples was screened in this study.
Results: About13% of the patients had FLT3 internal tandem duplications (ITDs), and 26% had D835 point mutation. FLT3 ITD mutation was associated with higher white blood cell (WBC) count at presentation and poor prognosis.
Conclusions: As the PML-RARA is not sufficient to develop APL, we assume FLT3 mutations and additional chromosomal alterations in this APL series may cooperate with PML-RARA in APL development. |
| first_indexed | 2024-03-12T05:46:53Z |
| format | Article |
| id | doaj.art-ca008dcbc6e6454eae6cbfa785db7ad8 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T05:46:53Z |
| publishDate | 2010-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-ca008dcbc6e6454eae6cbfa785db7ad82023-09-03T05:36:13ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072010-09-0143Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic LeukemiaMarjan Yaghmaie0Hossein Mozdarani1Kamran Alimoghaddam2Ardeshir Ghavamzadeh3Seyed Hamiollah Ghaffari4Medical Genetics Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranMedical Genetics Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University, Tehran, IranIntroduction: The secondary genetic changes other than the PML-RARA fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia (AML). However, the prognostic significance of FLT3 mutations in acute promyelocytic leukemia (APL) is not firmly established. Patients & Methods: FLT3 ITD screening by fragment length analysis and FLT3 D835 mutation by melting curve analysis in 23 APL samples was screened in this study. Results: About13% of the patients had FLT3 internal tandem duplications (ITDs), and 26% had D835 point mutation. FLT3 ITD mutation was associated with higher white blood cell (WBC) count at presentation and poor prognosis. Conclusions: As the PML-RARA is not sufficient to develop APL, we assume FLT3 mutations and additional chromosomal alterations in this APL series may cooperate with PML-RARA in APL development.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/253Acute promyelocytic leukemiaFLT3 tyrosine kinaseInternal tandem duplication |
| spellingShingle | Marjan Yaghmaie Hossein Mozdarani Kamran Alimoghaddam Ardeshir Ghavamzadeh Seyed Hamiollah Ghaffari Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia International Journal of Hematology-Oncology and Stem Cell Research Acute promyelocytic leukemia FLT3 tyrosine kinase Internal tandem duplication |
| title | Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia |
| title_full | Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia |
| title_fullStr | Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia |
| title_full_unstemmed | Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia |
| title_short | Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia |
| title_sort | evaluation of cytogenetic changes and flt3 mutations in patients with acute promyelocytic leukemia |
| topic | Acute promyelocytic leukemia FLT3 tyrosine kinase Internal tandem duplication |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/253 |
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