Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device
Here, we propose a microfluidic paper-based analytical device (µPAD) implemented with a near-field communication (NFC) tag as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The proposed method was based on the fact that Ag<sup>+</sup> could oxidize 3...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/267 |
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| author | Inmaculada Ortiz-Gómez Almudena Rivadeneyra José F. Salmerón Ignacio de Orbe-Payá Diego P. Morales Luis Fermín Capitán-Vallvey Alfonso Salinas-Castillo |
| author_facet | Inmaculada Ortiz-Gómez Almudena Rivadeneyra José F. Salmerón Ignacio de Orbe-Payá Diego P. Morales Luis Fermín Capitán-Vallvey Alfonso Salinas-Castillo |
| author_sort | Inmaculada Ortiz-Gómez |
| collection | DOAJ |
| description | Here, we propose a microfluidic paper-based analytical device (µPAD) implemented with a near-field communication (NFC) tag as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The proposed method was based on the fact that Ag<sup>+</sup> could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized blue TMB. Thus, the presence of GSH could cause the reduction of oxidized TMB, which resulted in a blue color fading. Based on this finding, we developed a method for the colorimetric determination of GSH using a smartphone. A µPAD implemented with the NFC tag allowed the harvesting of energy from a smartphone to activate the LED that allows the capture of a photograph of the µPAD by the smartphone. The integration between electronic interfaces into the hardware of digital image capture served as a means for quantitation. Importantly, this new method shows a low detection limit of 1.0 µM. Therefore, the most important features of this non-enzymatic method are high sensitivity and a simple, fast, portable and low-cost determination of GSH in just 20 min using a colorimetric signal. |
| first_indexed | 2024-03-11T09:05:20Z |
| format | Article |
| id | doaj.art-ca00bc73694c48dea38921fa9349e7fa |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:05:20Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ca00bc73694c48dea38921fa9349e7fa2023-11-16T19:26:28ZengMDPI AGBiosensors2079-63742023-02-0113226710.3390/bios13020267Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic DeviceInmaculada Ortiz-Gómez0Almudena Rivadeneyra1José F. Salmerón2Ignacio de Orbe-Payá3Diego P. Morales4Luis Fermín Capitán-Vallvey5Alfonso Salinas-Castillo6ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, SpainUnit of Excellence in Chemistry Applied to Biomedicine and the Environment, Faculty of Science, University of Granada, 18071 Granada, SpainUnit of Excellence in Chemistry Applied to Biomedicine and the Environment, Faculty of Science, University of Granada, 18071 Granada, SpainECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, SpainUnit of Excellence in Chemistry Applied to Biomedicine and the Environment, Faculty of Science, University of Granada, 18071 Granada, SpainECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, SpainECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, SpainHere, we propose a microfluidic paper-based analytical device (µPAD) implemented with a near-field communication (NFC) tag as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The proposed method was based on the fact that Ag<sup>+</sup> could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized blue TMB. Thus, the presence of GSH could cause the reduction of oxidized TMB, which resulted in a blue color fading. Based on this finding, we developed a method for the colorimetric determination of GSH using a smartphone. A µPAD implemented with the NFC tag allowed the harvesting of energy from a smartphone to activate the LED that allows the capture of a photograph of the µPAD by the smartphone. The integration between electronic interfaces into the hardware of digital image capture served as a means for quantitation. Importantly, this new method shows a low detection limit of 1.0 µM. Therefore, the most important features of this non-enzymatic method are high sensitivity and a simple, fast, portable and low-cost determination of GSH in just 20 min using a colorimetric signal.https://www.mdpi.com/2079-6374/13/2/267µPADNFC tagsmartphonecolorimetric assayglutathione determinationhealth prognosis |
| spellingShingle | Inmaculada Ortiz-Gómez Almudena Rivadeneyra José F. Salmerón Ignacio de Orbe-Payá Diego P. Morales Luis Fermín Capitán-Vallvey Alfonso Salinas-Castillo Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device Biosensors µPAD NFC tag smartphone colorimetric assay glutathione determination health prognosis |
| title | Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device |
| title_full | Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device |
| title_fullStr | Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device |
| title_full_unstemmed | Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device |
| title_short | Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device |
| title_sort | near field communication tag for colorimetric glutathione determination with a paper based microfluidic device |
| topic | µPAD NFC tag smartphone colorimetric assay glutathione determination health prognosis |
| url | https://www.mdpi.com/2079-6374/13/2/267 |
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