Murine cell glycolipids customization by modular expression of glycosyltransferases.
Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3683021?pdf=render |
| _version_ | 1818521564443836416 |
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| author | Emili Cid Miyako Yamamoto Marcus Buschbeck Fumiichiro Yamamoto |
| author_facet | Emili Cid Miyako Yamamoto Marcus Buschbeck Fumiichiro Yamamoto |
| author_sort | Emili Cid |
| collection | DOAJ |
| description | Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins. |
| first_indexed | 2024-12-11T01:52:45Z |
| format | Article |
| id | doaj.art-ca01d310a2df4b88bcf12a154b23ea46 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T01:52:45Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ca01d310a2df4b88bcf12a154b23ea462022-12-22T01:24:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6472810.1371/journal.pone.0064728Murine cell glycolipids customization by modular expression of glycosyltransferases.Emili CidMiyako YamamotoMarcus BuschbeckFumiichiro YamamotoFunctional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins.http://europepmc.org/articles/PMC3683021?pdf=render |
| spellingShingle | Emili Cid Miyako Yamamoto Marcus Buschbeck Fumiichiro Yamamoto Murine cell glycolipids customization by modular expression of glycosyltransferases. PLoS ONE |
| title | Murine cell glycolipids customization by modular expression of glycosyltransferases. |
| title_full | Murine cell glycolipids customization by modular expression of glycosyltransferases. |
| title_fullStr | Murine cell glycolipids customization by modular expression of glycosyltransferases. |
| title_full_unstemmed | Murine cell glycolipids customization by modular expression of glycosyltransferases. |
| title_short | Murine cell glycolipids customization by modular expression of glycosyltransferases. |
| title_sort | murine cell glycolipids customization by modular expression of glycosyltransferases |
| url | http://europepmc.org/articles/PMC3683021?pdf=render |
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