Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements
WRKY transcription factors regulate distinct parts of the plant defense transcriptome. Expression of many WRKY genes themselves is induced by pathogens or pathogen-mimicking molecules. Here, we demonstrate that Arabidopsis WRKY33 responds to various stimuli associated with plant defense as well as t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2007-04-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0420 |
| _version_ | 1811291289263538176 |
|---|---|
| author | Bernadette Lippok Rainer P. Birkenbihl Gaelle Rivory Janna Brümmer Elmon Schmelzer Elke Logemann Imre E. Somssich |
| author_facet | Bernadette Lippok Rainer P. Birkenbihl Gaelle Rivory Janna Brümmer Elmon Schmelzer Elke Logemann Imre E. Somssich |
| author_sort | Bernadette Lippok |
| collection | DOAJ |
| description | WRKY transcription factors regulate distinct parts of the plant defense transcriptome. Expression of many WRKY genes themselves is induced by pathogens or pathogen-mimicking molecules. Here, we demonstrate that Arabidopsis WRKY33 responds to various stimuli associated with plant defense as well as to different kinds of phytopathogens. Although rapid pathogen-induced AtWRKY33 expression does not require salicylic acid (SA) signaling, it is dependent on PAD4, a key regulator upstream of SA. Activation of AtWRKY33 is independent of de novo protein synthesis, suggesting that it is at least partly under negative regulatory control. We show that a set of three WRKY-specific cis-acting DNA elements (W boxes) within the AtWRKY33 promoter is required for efficient pathogen- or PAMP-triggered gene activation. This strongly indicates that WRKY transcription factors are major components of the regulatory machinery modulating immediate to early expression of this gene in response to pathogen attack. |
| first_indexed | 2024-04-13T04:27:12Z |
| format | Article |
| id | doaj.art-ca05babfb98d431da88915f6540ab6bb |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:12Z |
| publishDate | 2007-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-ca05babfb98d431da88915f6540ab6bb2022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-04-0120442042910.1094/MPMI-20-4-0420Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box ElementsBernadette LippokRainer P. BirkenbihlGaelle RivoryJanna BrümmerElmon SchmelzerElke LogemannImre E. SomssichWRKY transcription factors regulate distinct parts of the plant defense transcriptome. Expression of many WRKY genes themselves is induced by pathogens or pathogen-mimicking molecules. Here, we demonstrate that Arabidopsis WRKY33 responds to various stimuli associated with plant defense as well as to different kinds of phytopathogens. Although rapid pathogen-induced AtWRKY33 expression does not require salicylic acid (SA) signaling, it is dependent on PAD4, a key regulator upstream of SA. Activation of AtWRKY33 is independent of de novo protein synthesis, suggesting that it is at least partly under negative regulatory control. We show that a set of three WRKY-specific cis-acting DNA elements (W boxes) within the AtWRKY33 promoter is required for efficient pathogen- or PAMP-triggered gene activation. This strongly indicates that WRKY transcription factors are major components of the regulatory machinery modulating immediate to early expression of this gene in response to pathogen attack.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0420AlternariaHyaloperonospora parasiticapathogen-associated molecular patterns |
| spellingShingle | Bernadette Lippok Rainer P. Birkenbihl Gaelle Rivory Janna Brümmer Elmon Schmelzer Elke Logemann Imre E. Somssich Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements Molecular Plant-Microbe Interactions Alternaria Hyaloperonospora parasitica pathogen-associated molecular patterns |
| title | Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements |
| title_full | Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements |
| title_fullStr | Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements |
| title_full_unstemmed | Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements |
| title_short | Expression of AtWRKY33 Encoding a Pathogen- or PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements |
| title_sort | expression of atwrky33 encoding a pathogen or pamp responsive wrky transcription factor is regulated by a composite dna motif containing w box elements |
| topic | Alternaria Hyaloperonospora parasitica pathogen-associated molecular patterns |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0420 |
| work_keys_str_mv | AT bernadettelippok expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT rainerpbirkenbihl expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT gaellerivory expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT jannabrummer expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT elmonschmelzer expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT elkelogemann expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements AT imreesomssich expressionofatwrky33encodingapathogenorpampresponsivewrkytranscriptionfactorisregulatedbyacompositednamotifcontainingwboxelements |