Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications
Introduction: The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially in the long-term pulmonary complications. This study aims to investigate the association between post-COVID-19 pulmonary complications and mitochondrial regulatory proteins in...
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MDPI AG
2023-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/13/4253 |
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| author | Kamil Siekacz Anna Kumor-Kisielewska Joanna Miłkowska-Dymanowska Małgorzata Pietrusińska Krystian Bartczak Sebastian Majewski Adam Stańczyk Wojciech J. Piotrowski Adam J. Białas |
| author_facet | Kamil Siekacz Anna Kumor-Kisielewska Joanna Miłkowska-Dymanowska Małgorzata Pietrusińska Krystian Bartczak Sebastian Majewski Adam Stańczyk Wojciech J. Piotrowski Adam J. Białas |
| author_sort | Kamil Siekacz |
| collection | DOAJ |
| description | Introduction: The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially in the long-term pulmonary complications. This study aims to investigate the association between post-COVID-19 pulmonary complications and mitochondrial regulatory proteins in the context of oxidative stress. Methodology: Patients who had recovered from COVID-19 were enrolled. According to the evidence of persistent interstitial lung lesions on computed tomography (CT), patients were divided into a long-term pulmonary complications group (P(+)) and a control group without long-term pulmonary complications (P(−)). We randomly selected 80 patients for investigation (40 subjects for each group). Biomarkers levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: The serum concentrations of mitochondrial regulatory proteins were significantly higher in the P(+) group, including PTEN-induced kinase 1 (PINK1): 1.62 [1.02–2.29] ng/mL vs. 1.34 [0.94–1.74] ng/mL (<i>p</i> = 0.046); Dynamin-1-like protein (DNM1L): 1.6 [0.9–2.4] ng/mL IQR vs. 0.9 [0.5–1.6] ng/mL (<i>p</i> = 0.004); and Mitofusin-2 (MFN2): 0.3 [0.2–0.5] ng/mL vs. 0.2 [0.1–0.3] ng/mL IQR (<i>p</i> = 0.001). Patients from the P(+) group also had higher serum levels of chemokine ligand 18 (PARC, CCL18), IL-6, and tumour necrosis factor-alpha (TNF-α) cytokines than the P(−) group. The concentration of interferon alpha (IFN-α) was decreased in the P(+) group. Furthermore, we observed statistically significant correlations between the advanced glycation end product (sRAGE) and TNF-α (Pearson’s factor R = 0.637; <i>p</i> < 0.001) and between serum levels of DNM1L and IFN-α (Pearson’s factor R = 0.501; <i>p</i> = 0.002) in P(+) patients. Conclusions: Elevated concentrations of mitochondrial biomarkers in post-COVID-19 patients with long-term pulmonary complications indicate their possible role in the pathobiology of COVID-19 pulmonary sequelae. Oxidative stress is associated with the immune response and inflammation after COVID-19. TNF-α could be a promising biomarker for predicting pulmonary complications and may be a potential target for therapeutic intervention in patients with post-COVID-19 complications. |
| first_indexed | 2024-03-11T01:37:31Z |
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| id | doaj.art-ca0937979c39464fb1a3dbbf6b81172d |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T01:37:31Z |
| publishDate | 2023-06-01 |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-ca0937979c39464fb1a3dbbf6b81172d2023-11-18T16:51:14ZengMDPI AGJournal of Clinical Medicine2077-03832023-06-011213425310.3390/jcm12134253Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 ComplicationsKamil Siekacz0Anna Kumor-Kisielewska1Joanna Miłkowska-Dymanowska2Małgorzata Pietrusińska3Krystian Bartczak4Sebastian Majewski5Adam Stańczyk6Wojciech J. Piotrowski7Adam J. Białas8Department of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Clinical Pharmacology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandDepartment of Pneumology, Medical University of Lodz, 90-419 Lodz, PolandIntroduction: The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially in the long-term pulmonary complications. This study aims to investigate the association between post-COVID-19 pulmonary complications and mitochondrial regulatory proteins in the context of oxidative stress. Methodology: Patients who had recovered from COVID-19 were enrolled. According to the evidence of persistent interstitial lung lesions on computed tomography (CT), patients were divided into a long-term pulmonary complications group (P(+)) and a control group without long-term pulmonary complications (P(−)). We randomly selected 80 patients for investigation (40 subjects for each group). Biomarkers levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: The serum concentrations of mitochondrial regulatory proteins were significantly higher in the P(+) group, including PTEN-induced kinase 1 (PINK1): 1.62 [1.02–2.29] ng/mL vs. 1.34 [0.94–1.74] ng/mL (<i>p</i> = 0.046); Dynamin-1-like protein (DNM1L): 1.6 [0.9–2.4] ng/mL IQR vs. 0.9 [0.5–1.6] ng/mL (<i>p</i> = 0.004); and Mitofusin-2 (MFN2): 0.3 [0.2–0.5] ng/mL vs. 0.2 [0.1–0.3] ng/mL IQR (<i>p</i> = 0.001). Patients from the P(+) group also had higher serum levels of chemokine ligand 18 (PARC, CCL18), IL-6, and tumour necrosis factor-alpha (TNF-α) cytokines than the P(−) group. The concentration of interferon alpha (IFN-α) was decreased in the P(+) group. Furthermore, we observed statistically significant correlations between the advanced glycation end product (sRAGE) and TNF-α (Pearson’s factor R = 0.637; <i>p</i> < 0.001) and between serum levels of DNM1L and IFN-α (Pearson’s factor R = 0.501; <i>p</i> = 0.002) in P(+) patients. Conclusions: Elevated concentrations of mitochondrial biomarkers in post-COVID-19 patients with long-term pulmonary complications indicate their possible role in the pathobiology of COVID-19 pulmonary sequelae. Oxidative stress is associated with the immune response and inflammation after COVID-19. TNF-α could be a promising biomarker for predicting pulmonary complications and may be a potential target for therapeutic intervention in patients with post-COVID-19 complications.https://www.mdpi.com/2077-0383/12/13/4253SARS-CoV-2post-COVID-19PINK1DNM1LCCL18TNF-α |
| spellingShingle | Kamil Siekacz Anna Kumor-Kisielewska Joanna Miłkowska-Dymanowska Małgorzata Pietrusińska Krystian Bartczak Sebastian Majewski Adam Stańczyk Wojciech J. Piotrowski Adam J. Białas Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications Journal of Clinical Medicine SARS-CoV-2 post-COVID-19 PINK1 DNM1L CCL18 TNF-α |
| title | Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications |
| title_full | Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications |
| title_fullStr | Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications |
| title_full_unstemmed | Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications |
| title_short | Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications |
| title_sort | oxidative biomarkers associated with the pulmonary manifestation of post covid 19 complications |
| topic | SARS-CoV-2 post-COVID-19 PINK1 DNM1L CCL18 TNF-α |
| url | https://www.mdpi.com/2077-0383/12/13/4253 |
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