Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments
The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2008-12-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200812-0003_simple-method-of-open-skin-wound-healing-model-in-corticosteroid-treated-and-diabetic-rats-standardization-of.php |
| _version_ | 1797897686737747968 |
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| author | P. Gal R. Kilik M. Mokry B. Vidinsky T. Vasilenko S. Mozes N. Bobrov Z. Tomori J. Bober L. Lenhardt |
| author_facet | P. Gal R. Kilik M. Mokry B. Vidinsky T. Vasilenko S. Mozes N. Bobrov Z. Tomori J. Bober L. Lenhardt |
| author_sort | P. Gal |
| collection | DOAJ |
| description | The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment. |
| first_indexed | 2024-04-10T08:02:16Z |
| format | Article |
| id | doaj.art-ca097cf9c2f748c6ad21618018b331d9 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:16Z |
| publishDate | 2008-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-ca097cf9c2f748c6ad21618018b331d92023-02-23T03:49:34ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922008-12-01531265265910.17221/1973-VETMEDvet-200812-0003Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessmentsP. Gal0R. Kilik1M. Mokry2B. Vidinsky3T. Vasilenko4S. Mozes5N. Bobrov6Z. Tomori7J. Bober8L. Lenhardt9Pavol Jozef Safarik University, Kosice, Slovak Republic1st Clinic of Surgery, Pavol Jozef Safarik University and L. Pasteur Faculty Hospital, Kosice, Slovak RepublicPavol Jozef Safarik University, Kosice, Slovak RepublicPavol Jozef Safarik University, Kosice, Slovak RepublicPavol Jozef Safarik University, Kosice, Slovak RepublicInstitute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovak RepublicPavol Jozef Safarik University, Kosice, Slovak RepublicInstitute of Experimental Physics, Slovak Academy of Sciences, Kosice, Slovak Republic1st Clinic of Surgery, Pavol Jozef Safarik University and L. Pasteur Faculty Hospital, Kosice, Slovak RepublicUniversity of Veterinary Medicine, Kosice, Slovak RepublicThe use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.https://vetmed.agriculturejournals.cz/artkey/vet-200812-0003_simple-method-of-open-skin-wound-healing-model-in-corticosteroid-treated-and-diabetic-rats-standardization-of.phprepairregenerationscar tissueinsulin dependent diabetes mellitus |
| spellingShingle | P. Gal R. Kilik M. Mokry B. Vidinsky T. Vasilenko S. Mozes N. Bobrov Z. Tomori J. Bober L. Lenhardt Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments Veterinární Medicína repair regeneration scar tissue insulin dependent diabetes mellitus |
| title | Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments |
| title_full | Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments |
| title_fullStr | Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments |
| title_full_unstemmed | Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments |
| title_short | Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments |
| title_sort | simple method of open skin wound healing model in corticosteroid treated and diabetic rats standardization of semi quantitative and quantitative histological assessments |
| topic | repair regeneration scar tissue insulin dependent diabetes mellitus |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200812-0003_simple-method-of-open-skin-wound-healing-model-in-corticosteroid-treated-and-diabetic-rats-standardization-of.php |
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