Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates
Data on the prevalence of MCR-producing <i>Enterobacterales</i> of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/3/305 |
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| author | Ágnes Sonnevend Walid Q. Alali Sara A. Mahmoud Akela Ghazawi Greeshma Bharathan Szilvia Melegh Tahir A. Rizvi Tibor Pál |
| author_facet | Ágnes Sonnevend Walid Q. Alali Sara A. Mahmoud Akela Ghazawi Greeshma Bharathan Szilvia Melegh Tahir A. Rizvi Tibor Pál |
| author_sort | Ágnes Sonnevend |
| collection | DOAJ |
| description | Data on the prevalence of MCR-producing <i>Enterobacterales</i> of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composite samples per farm grown on colistin-supplemented plates were PCR-screened for alleles of the <i>mcr</i> gene. Thirty-nine isolates selected based on species, colony morphology, and plasmid profile were subjected to whole genome sequencing. The panel of their resistance and virulence genes, MLST and cgMLST were established. Transferability and incompatibility types of the MCR-plasmids were determined. <i>mcr-1.1</i> positive strains were identified in 36 of the 40 samples. Thirty-four multi-drug resistant <i>Escherichia coli</i> of 16 different sequence types, two <i>Escherichia albertii</i>, two <i>Klebsiella pneumoniae</i> and one <i>Salmonella minnesota</i> were identified. Beyond various aminoglycoside, tetracycline, and co-trimoxazole resistance genes, seven of them also carried ESBL genes and one <i>bla</i><sub>CMY-2</sub>. Six IncHI2, 26 IncI2 and 4 IncX4 MCR-plasmids were mobilized, in case of the IncHI2 plasmids co-transferring ampicillin, chloramphenicol and tetracycline resistance. The diversity of <i>mcr-1</i> positive strains suggest a complex local epidemiology calling for a coordinated surveillance including animals, retail meat and clinical cases. |
| first_indexed | 2024-03-09T20:12:58Z |
| format | Article |
| id | doaj.art-ca0a18bc90df40629498a3cbbc2d9fe7 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:12:58Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ca0a18bc90df40629498a3cbbc2d9fe72023-11-24T00:10:25ZengMDPI AGAntibiotics2079-63822022-02-0111330510.3390/antibiotics11030305Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab EmiratesÁgnes Sonnevend0Walid Q. Alali1Sara A. Mahmoud2Akela Ghazawi3Greeshma Bharathan4Szilvia Melegh5Tahir A. Rizvi6Tibor Pál7Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 7624 Pécs, HungaryDepartment of Epidemiology and Biostatistics, Faculty of Public Health, Kuwait University, Safat, Kuwait City 13110, KuwaitDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, 7624 Pécs, HungaryDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, 7624 Pécs, HungaryData on the prevalence of MCR-producing <i>Enterobacterales</i> of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composite samples per farm grown on colistin-supplemented plates were PCR-screened for alleles of the <i>mcr</i> gene. Thirty-nine isolates selected based on species, colony morphology, and plasmid profile were subjected to whole genome sequencing. The panel of their resistance and virulence genes, MLST and cgMLST were established. Transferability and incompatibility types of the MCR-plasmids were determined. <i>mcr-1.1</i> positive strains were identified in 36 of the 40 samples. Thirty-four multi-drug resistant <i>Escherichia coli</i> of 16 different sequence types, two <i>Escherichia albertii</i>, two <i>Klebsiella pneumoniae</i> and one <i>Salmonella minnesota</i> were identified. Beyond various aminoglycoside, tetracycline, and co-trimoxazole resistance genes, seven of them also carried ESBL genes and one <i>bla</i><sub>CMY-2</sub>. Six IncHI2, 26 IncI2 and 4 IncX4 MCR-plasmids were mobilized, in case of the IncHI2 plasmids co-transferring ampicillin, chloramphenicol and tetracycline resistance. The diversity of <i>mcr-1</i> positive strains suggest a complex local epidemiology calling for a coordinated surveillance including animals, retail meat and clinical cases.https://www.mdpi.com/2079-6382/11/3/305mobile colistin resistancepoultrymulti-drug resistanceMiddle East |
| spellingShingle | Ágnes Sonnevend Walid Q. Alali Sara A. Mahmoud Akela Ghazawi Greeshma Bharathan Szilvia Melegh Tahir A. Rizvi Tibor Pál Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates Antibiotics mobile colistin resistance poultry multi-drug resistance Middle East |
| title | Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates |
| title_full | Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates |
| title_fullStr | Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates |
| title_full_unstemmed | Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates |
| title_short | Molecular Characterization of <i>MCR-1</i> Producing <i>Enterobacterales</i> Isolated in Poultry Farms in the United Arab Emirates |
| title_sort | molecular characterization of i mcr 1 i producing i enterobacterales i isolated in poultry farms in the united arab emirates |
| topic | mobile colistin resistance poultry multi-drug resistance Middle East |
| url | https://www.mdpi.com/2079-6382/11/3/305 |
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