Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors
In veterinary medicine, the issue of antimicrobial resistance was mainly addressed in food-producing animals (although companion animals also deserve attention). Indeed, these species may be reservoir of resistant microorganisms, such as extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing b...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/10/1251 |
| _version_ | 1827680352885276672 |
|---|---|
| author | Nicoletta Formenti Andrea Grassi Giovanni Parisio Claudia Romeo Flavia Guarneri Laura Birbes Alessandra Pitozzi Federico Scali Antonio Marco Maisano Maria Beatrice Boniotti Paolo Pasquali Giovanni Loris Alborali |
| author_facet | Nicoletta Formenti Andrea Grassi Giovanni Parisio Claudia Romeo Flavia Guarneri Laura Birbes Alessandra Pitozzi Federico Scali Antonio Marco Maisano Maria Beatrice Boniotti Paolo Pasquali Giovanni Loris Alborali |
| author_sort | Nicoletta Formenti |
| collection | DOAJ |
| description | In veterinary medicine, the issue of antimicrobial resistance was mainly addressed in food-producing animals (although companion animals also deserve attention). Indeed, these species may be reservoir of resistant microorganisms, such as extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing bacteria. Dogs in particular may transmit them to close-contact humans. Overall 266 faecal samples of healthy dogs were microbiologically and molecularly analyzed to investigate ESBL/AmpC-producing <i>Escherichia coli</i> and the effects of host and environmental factors on their spread. A prevalence of 25.9% of ESBL/AmpC-producing <i>E. coli</i>, supported by <i>bla</i><sub>CTX-M</sub> (79.7%), <i>bla</i><sub>TEM</sub> (47.8%), <i>bla</i><sub>CMY</sub> (13%), and <i>bla</i><sub>SHV</sub> (5.8%) gene detection, emerged. Dogs frequenting extra-urban environments showed significantly higher odds of being positive to ESBL/AmpC <i>E. coli</i> (30.2%) compared to urban dogs (16.7%) identifying the environment as a risk factor. About 88.4% of isolates were resistant to cephalosporins, 8.7% to cephalosporins and carbapenems, and 2.9% to cephalosporins, carbapenems, and penicillins. ESBL/AmpC-producing <i>E. coli</i> expressing <i>bla</i><sub>CMY</sub> were significantly more resistant to cefoxitin, cefotaxime/clavulanic acid and ceftazidime/clavulanic acid, highlighting its negative effects. Our results suggest the role of domestic dogs as a maintenance host of ESBL/AmpC-producing <i>E. coli</i> leading to a constant health monitoring. The recorded resistances to carbapenems implies attention and further investigations. |
| first_indexed | 2024-03-10T06:46:40Z |
| format | Article |
| id | doaj.art-ca0cbdf7683c428b80e0681bdf32e85e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T06:46:40Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ca0cbdf7683c428b80e0681bdf32e85e2023-11-22T17:14:30ZengMDPI AGAntibiotics2079-63822021-10-011010125110.3390/antibiotics10101251Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk FactorsNicoletta Formenti0Andrea Grassi1Giovanni Parisio2Claudia Romeo3Flavia Guarneri4Laura Birbes5Alessandra Pitozzi6Federico Scali7Antonio Marco Maisano8Maria Beatrice Boniotti9Paolo Pasquali10Giovanni Loris Alborali11Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyDipartimento di Scienze degli Alimenti e del Farmaco, Università di Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyDipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyIn veterinary medicine, the issue of antimicrobial resistance was mainly addressed in food-producing animals (although companion animals also deserve attention). Indeed, these species may be reservoir of resistant microorganisms, such as extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing bacteria. Dogs in particular may transmit them to close-contact humans. Overall 266 faecal samples of healthy dogs were microbiologically and molecularly analyzed to investigate ESBL/AmpC-producing <i>Escherichia coli</i> and the effects of host and environmental factors on their spread. A prevalence of 25.9% of ESBL/AmpC-producing <i>E. coli</i>, supported by <i>bla</i><sub>CTX-M</sub> (79.7%), <i>bla</i><sub>TEM</sub> (47.8%), <i>bla</i><sub>CMY</sub> (13%), and <i>bla</i><sub>SHV</sub> (5.8%) gene detection, emerged. Dogs frequenting extra-urban environments showed significantly higher odds of being positive to ESBL/AmpC <i>E. coli</i> (30.2%) compared to urban dogs (16.7%) identifying the environment as a risk factor. About 88.4% of isolates were resistant to cephalosporins, 8.7% to cephalosporins and carbapenems, and 2.9% to cephalosporins, carbapenems, and penicillins. ESBL/AmpC-producing <i>E. coli</i> expressing <i>bla</i><sub>CMY</sub> were significantly more resistant to cefoxitin, cefotaxime/clavulanic acid and ceftazidime/clavulanic acid, highlighting its negative effects. Our results suggest the role of domestic dogs as a maintenance host of ESBL/AmpC-producing <i>E. coli</i> leading to a constant health monitoring. The recorded resistances to carbapenems implies attention and further investigations.https://www.mdpi.com/2079-6382/10/10/1251extra-urban environments<i>bla</i><sub>CMY</sub>cefotaxime/clavulanic acidceftazidime/clavulanic acid<i>bla</i><sub>CTX-M</sub>cefoxitin |
| spellingShingle | Nicoletta Formenti Andrea Grassi Giovanni Parisio Claudia Romeo Flavia Guarneri Laura Birbes Alessandra Pitozzi Federico Scali Antonio Marco Maisano Maria Beatrice Boniotti Paolo Pasquali Giovanni Loris Alborali Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors Antibiotics extra-urban environments <i>bla</i><sub>CMY</sub> cefotaxime/clavulanic acid ceftazidime/clavulanic acid <i>bla</i><sub>CTX-M</sub> cefoxitin |
| title | Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors |
| title_full | Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors |
| title_fullStr | Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors |
| title_full_unstemmed | Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors |
| title_short | Extended-Spectrum-β-Lactamase- and AmpC-Producing <i>Escherichia coli</i> in Domestic Dogs: Spread, Characterisation and Associated Risk Factors |
| title_sort | extended spectrum β lactamase and ampc producing i escherichia coli i in domestic dogs spread characterisation and associated risk factors |
| topic | extra-urban environments <i>bla</i><sub>CMY</sub> cefotaxime/clavulanic acid ceftazidime/clavulanic acid <i>bla</i><sub>CTX-M</sub> cefoxitin |
| url | https://www.mdpi.com/2079-6382/10/10/1251 |
| work_keys_str_mv | AT nicolettaformenti extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT andreagrassi extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT giovanniparisio extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT claudiaromeo extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT flaviaguarneri extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT laurabirbes extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT alessandrapitozzi extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT federicoscali extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT antoniomarcomaisano extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT mariabeatriceboniotti extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT paolopasquali extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors AT giovannilorisalborali extendedspectrumblactamaseandampcproducingiescherichiacoliiindomesticdogsspreadcharacterisationandassociatedriskfactors |