Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>)
<i>Cnidium officinale Makino</i>, a perennial crop in the <i>Umbeliperae</i> family, is one of Korea’s representative forest medicinal plants. However, the growing area of <i>C. officinale</i> has been reduced by plant disease and soil sickness caused by fusarium...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1555 |
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| author | Seok Hui Lee Su Hong Jeon Jun Young Park Dae Sol Kim Ji Ah Kim Hui Yeong Jeong Jun Won Kang |
| author_facet | Seok Hui Lee Su Hong Jeon Jun Young Park Dae Sol Kim Ji Ah Kim Hui Yeong Jeong Jun Won Kang |
| author_sort | Seok Hui Lee |
| collection | DOAJ |
| description | <i>Cnidium officinale Makino</i>, a perennial crop in the <i>Umbeliperae</i> family, is one of Korea’s representative forest medicinal plants. However, the growing area of <i>C. officinale</i> has been reduced by plant disease and soil sickness caused by fusarium wilt. This study isolated rhizosphere bacteria from <i>C. officinale</i>, and their antagonistic activity was evaluated against <i>Fusarium solani.</i> Particularly, four isolated strains, namely, PT1, ST7, ST8, and SP4, showed a significant antagonistic activity against <i>F. solani</i>. An in planta test showed that the mortality rates of shoots were significantly low in the PT1-inoculated group. The fresh and dry weights of the inoculated plants were also higher than that of the other groups. The 16S rRNA gene sequencing identified the strain PT1 as <i>Leclercia adecarboxylata</i>, and downstream studies confirmed the production of antagonism-related enzymes such as siderophore and N-acetyl-β-glucosaminidase. The phosphorous solubilizing ability and secretion of related enzymes were also analyzed. The results showed that PT1 strain could be utilized as promising plant growth-promoting rhizobacteria (PGPR) and biocontrol agents (BCAs). |
| first_indexed | 2024-03-11T02:08:39Z |
| format | Article |
| id | doaj.art-ca11787d05b34068aac0e67294fd6454 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:39Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ca11787d05b34068aac0e67294fd64542023-11-18T11:43:31ZengMDPI AGMicroorganisms2076-26072023-06-01116155510.3390/microorganisms11061555Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>)Seok Hui Lee0Su Hong Jeon1Jun Young Park2Dae Sol Kim3Ji Ah Kim4Hui Yeong Jeong5Jun Won Kang6Department of Forestry, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Forestry, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Forestry, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Forestry, Kyungpook National University, Daegu 41566, Republic of KoreaForest Medicinal Resource Research Center, NIFoS, Yeongju 36040, Republic of KoreaForest Medicinal Resource Research Center, NIFoS, Yeongju 36040, Republic of KoreaDepartment of Forestry, Kyungpook National University, Daegu 41566, Republic of Korea<i>Cnidium officinale Makino</i>, a perennial crop in the <i>Umbeliperae</i> family, is one of Korea’s representative forest medicinal plants. However, the growing area of <i>C. officinale</i> has been reduced by plant disease and soil sickness caused by fusarium wilt. This study isolated rhizosphere bacteria from <i>C. officinale</i>, and their antagonistic activity was evaluated against <i>Fusarium solani.</i> Particularly, four isolated strains, namely, PT1, ST7, ST8, and SP4, showed a significant antagonistic activity against <i>F. solani</i>. An in planta test showed that the mortality rates of shoots were significantly low in the PT1-inoculated group. The fresh and dry weights of the inoculated plants were also higher than that of the other groups. The 16S rRNA gene sequencing identified the strain PT1 as <i>Leclercia adecarboxylata</i>, and downstream studies confirmed the production of antagonism-related enzymes such as siderophore and N-acetyl-β-glucosaminidase. The phosphorous solubilizing ability and secretion of related enzymes were also analyzed. The results showed that PT1 strain could be utilized as promising plant growth-promoting rhizobacteria (PGPR) and biocontrol agents (BCAs).https://www.mdpi.com/2076-2607/11/6/1555<i>Bacillus</i><i>Cnidium officinale</i><i>Fusarium solani</i>fusarium wilt<i>Leclercia adecarboxylata</i>PGPR |
| spellingShingle | Seok Hui Lee Su Hong Jeon Jun Young Park Dae Sol Kim Ji Ah Kim Hui Yeong Jeong Jun Won Kang Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) Microorganisms <i>Bacillus</i> <i>Cnidium officinale</i> <i>Fusarium solani</i> fusarium wilt <i>Leclercia adecarboxylata</i> PGPR |
| title | Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) |
| title_full | Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) |
| title_fullStr | Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) |
| title_full_unstemmed | Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) |
| title_short | Isolation and Evaluation of the Antagonistic Activity of <i>Cnidium officinale</i> Rhizosphere Bacteria against Phytopathogenic fungi (<i>Fusarium solani</i>) |
| title_sort | isolation and evaluation of the antagonistic activity of i cnidium officinale i rhizosphere bacteria against phytopathogenic fungi i fusarium solani i |
| topic | <i>Bacillus</i> <i>Cnidium officinale</i> <i>Fusarium solani</i> fusarium wilt <i>Leclercia adecarboxylata</i> PGPR |
| url | https://www.mdpi.com/2076-2607/11/6/1555 |
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