Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results
A newly introduced combination of intraperitoneal dehydration and hyperthermia has recently been shown to be feasible and cytotoxic for colon cancer cells in vivo. For the first time, our study now aims to evaluate dehydration under hyperthermic conditions combined with chemotherapy for potential us...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1424-8247/16/5/763 |
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| author | Carolina Khosrawipour Agata Diakun Shiri Li Hien Lau Joanna Kulas Veria Khosrawipour Wojciech Kielan Agata Mikolajczyk-Martinez |
| author_facet | Carolina Khosrawipour Agata Diakun Shiri Li Hien Lau Joanna Kulas Veria Khosrawipour Wojciech Kielan Agata Mikolajczyk-Martinez |
| author_sort | Carolina Khosrawipour |
| collection | DOAJ |
| description | A newly introduced combination of intraperitoneal dehydration and hyperthermia has recently been shown to be feasible and cytotoxic for colon cancer cells in vivo. For the first time, our study now aims to evaluate dehydration under hyperthermic conditions combined with chemotherapy for potential use in the clinical setting. In this study, in vitro colon cancer cells (HT-29) were subjected to single or several cycles of partial dehydration under hyperthermic conditions (45 °C), followed by chemotherapy (triple exposure) with oxaliplatin or doxorubicin in various configurations. The viability, cytotoxicity, and proliferation of cells after the proposed protocols were assessed. Intracellular doxorubicin uptake was measured via flow cytometry. After one cycle of triple exposure, the viability of HT-29 cells was significantly reduced versus the untreated control (65.11 ± 5%, <i>p</i> < 0.0001) and versus only chemotherapy (61.2 ± 7%, <i>p</i> < 0.0001). An increased chemotherapeutic inflow into the cells after triple exposure was detected (53.4 ± 11%) when compared to cells treated with chemotherapy alone (34.23 ± 10%) (<i>p</i> < 0.001). Partial dehydration in a hyperthermic condition combined with chemotherapy increases the overall cytotoxicity of colon cancer cells significantly compared to chemotherapy alone. This could possibly be related to enhanced intracellular uptake of chemotherapeutic agents after partial dehydration. Further studies are required for the further evaluation of this new concept. |
| first_indexed | 2024-03-11T03:24:44Z |
| format | Article |
| id | doaj.art-ca1260e9e9244af2b8833fc7795cb45e |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T03:24:44Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ca1260e9e9244af2b8833fc7795cb45e2023-11-18T02:49:36ZengMDPI AGPharmaceuticals1424-82472023-05-0116576310.3390/ph16050763Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro ResultsCarolina Khosrawipour0Agata Diakun1Shiri Li2Hien Lau3Joanna Kulas4Veria Khosrawipour5Wojciech Kielan6Agata Mikolajczyk-Martinez7Faculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, Poland2nd Department of General Surgery and Surgical Oncology, Wroclaw Medical University, 50-367 Wroclaw, PolandDivision of Colon and Rectal Surgery, Department of Surgery, New York Presbyterian Hospital-Weill Cornell College of Medicine, New York, NY 10065, USADepartment of Surgery, University of California Irvine (UCI)—Medical Center, Irvine, CA 92868, USAFaculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland2nd Department of General Surgery and Surgical Oncology, Wroclaw Medical University, 50-367 Wroclaw, Poland2nd Department of General Surgery and Surgical Oncology, Wroclaw Medical University, 50-367 Wroclaw, PolandDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, PolandA newly introduced combination of intraperitoneal dehydration and hyperthermia has recently been shown to be feasible and cytotoxic for colon cancer cells in vivo. For the first time, our study now aims to evaluate dehydration under hyperthermic conditions combined with chemotherapy for potential use in the clinical setting. In this study, in vitro colon cancer cells (HT-29) were subjected to single or several cycles of partial dehydration under hyperthermic conditions (45 °C), followed by chemotherapy (triple exposure) with oxaliplatin or doxorubicin in various configurations. The viability, cytotoxicity, and proliferation of cells after the proposed protocols were assessed. Intracellular doxorubicin uptake was measured via flow cytometry. After one cycle of triple exposure, the viability of HT-29 cells was significantly reduced versus the untreated control (65.11 ± 5%, <i>p</i> < 0.0001) and versus only chemotherapy (61.2 ± 7%, <i>p</i> < 0.0001). An increased chemotherapeutic inflow into the cells after triple exposure was detected (53.4 ± 11%) when compared to cells treated with chemotherapy alone (34.23 ± 10%) (<i>p</i> < 0.001). Partial dehydration in a hyperthermic condition combined with chemotherapy increases the overall cytotoxicity of colon cancer cells significantly compared to chemotherapy alone. This could possibly be related to enhanced intracellular uptake of chemotherapeutic agents after partial dehydration. Further studies are required for the further evaluation of this new concept.https://www.mdpi.com/1424-8247/16/5/763peritoneal metastasiscolorectal cancerintraperitoneal chemotherapydehydrationhyperthermiaoxaliplatin |
| spellingShingle | Carolina Khosrawipour Agata Diakun Shiri Li Hien Lau Joanna Kulas Veria Khosrawipour Wojciech Kielan Agata Mikolajczyk-Martinez Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results Pharmaceuticals peritoneal metastasis colorectal cancer intraperitoneal chemotherapy dehydration hyperthermia oxaliplatin |
| title | Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results |
| title_full | Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results |
| title_fullStr | Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results |
| title_full_unstemmed | Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results |
| title_short | Triple-Therapy of Peritoneal Metastasis—Partial-Dehydration under Hyperthermic Condition Combined with Chemotherapy: The First Preliminary In-Vitro Results |
| title_sort | triple therapy of peritoneal metastasis partial dehydration under hyperthermic condition combined with chemotherapy the first preliminary in vitro results |
| topic | peritoneal metastasis colorectal cancer intraperitoneal chemotherapy dehydration hyperthermia oxaliplatin |
| url | https://www.mdpi.com/1424-8247/16/5/763 |
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