Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva
The spread of viruses among cells and hosts often involves multi-virion structures. For instance, virions can form aggregates that allow for the co-delivery of multiple genome copies to the same cell from a single infectious unit. Previously, we showed that vesicular stomatitis virus (VSV), an envel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/3/282 |
| _version_ | 1829099468436275200 |
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| author | Valesca Anschau Rafael Sanjuán |
| author_facet | Valesca Anschau Rafael Sanjuán |
| author_sort | Valesca Anschau |
| collection | DOAJ |
| description | The spread of viruses among cells and hosts often involves multi-virion structures. For instance, virions can form aggregates that allow for the co-delivery of multiple genome copies to the same cell from a single infectious unit. Previously, we showed that vesicular stomatitis virus (VSV), an enveloped, negative-strand RNA virus, undergoes strong aggregation in the presence of saliva from certain individuals. However, the molecular components responsible for such aggregation remain unknown. Here we show that saliva-driven aggregation is protein dependent, and we use comparative proteomics to analyze the protein content of strongly versus poorly aggregating saliva. Quantitative analysis of over 300 proteins led to the identification of 18 upregulated proteins in strongly aggregating saliva. One of these proteins, the fibrinogen gamma chain, was verified experimentally as a factor promoting VSV aggregation in a dose-dependent manner. This study hence identifies a protein responsible for saliva-driven VSV aggregation. Yet, the possible involvement of additional proteins or factors cannot be discarded. |
| first_indexed | 2024-12-10T22:18:46Z |
| format | Article |
| id | doaj.art-ca132885964e420ea310b406de73404c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T22:18:46Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ca132885964e420ea310b406de73404c2022-12-22T01:31:23ZengMDPI AGViruses1999-49152020-03-0112328210.3390/v12030282v12030282Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in SalivaValesca Anschau0Rafael Sanjuán1Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980 València, SpainInstitute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980 València, SpainThe spread of viruses among cells and hosts often involves multi-virion structures. For instance, virions can form aggregates that allow for the co-delivery of multiple genome copies to the same cell from a single infectious unit. Previously, we showed that vesicular stomatitis virus (VSV), an enveloped, negative-strand RNA virus, undergoes strong aggregation in the presence of saliva from certain individuals. However, the molecular components responsible for such aggregation remain unknown. Here we show that saliva-driven aggregation is protein dependent, and we use comparative proteomics to analyze the protein content of strongly versus poorly aggregating saliva. Quantitative analysis of over 300 proteins led to the identification of 18 upregulated proteins in strongly aggregating saliva. One of these proteins, the fibrinogen gamma chain, was verified experimentally as a factor promoting VSV aggregation in a dose-dependent manner. This study hence identifies a protein responsible for saliva-driven VSV aggregation. Yet, the possible involvement of additional proteins or factors cannot be discarded.https://www.mdpi.com/1999-4915/12/3/282vesicular stomatitis viruscollective infectious unitsviral transmissionvirion aggregationfibrinogenlabel-free proteomics |
| spellingShingle | Valesca Anschau Rafael Sanjuán Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva Viruses vesicular stomatitis virus collective infectious units viral transmission virion aggregation fibrinogen label-free proteomics |
| title | Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva |
| title_full | Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva |
| title_fullStr | Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva |
| title_full_unstemmed | Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva |
| title_short | Fibrinogen Gamma Chain Promotes Aggregation of Vesicular Stomatitis Virus in Saliva |
| title_sort | fibrinogen gamma chain promotes aggregation of vesicular stomatitis virus in saliva |
| topic | vesicular stomatitis virus collective infectious units viral transmission virion aggregation fibrinogen label-free proteomics |
| url | https://www.mdpi.com/1999-4915/12/3/282 |
| work_keys_str_mv | AT valescaanschau fibrinogengammachainpromotesaggregationofvesicularstomatitisvirusinsaliva AT rafaelsanjuan fibrinogengammachainpromotesaggregationofvesicularstomatitisvirusinsaliva |