The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae
Heavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium—Cr(VI) on photosynthesis and lipid accumulation in <i>Mucidosphaerium pulchellum</i> and <i>Micract...
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MDPI AG
2021-04-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/14/8/2260 |
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| author | Khawaja Muhammad Imran Bashir Hyeon-Jun Lee Sana Mansoor Alexander Jahn Man-Gi Cho |
| author_facet | Khawaja Muhammad Imran Bashir Hyeon-Jun Lee Sana Mansoor Alexander Jahn Man-Gi Cho |
| author_sort | Khawaja Muhammad Imran Bashir |
| collection | DOAJ |
| description | Heavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium—Cr(VI) on photosynthesis and lipid accumulation in <i>Mucidosphaerium pulchellum</i> and <i>Micractinium pusillum</i> exposed to different concentrations (0–500 μg L<sup>−1</sup>) was investigated for 11 days. A significant (<i>p</i> < 0.05) increase in lipid content was observed with increasing Cr(VI) concentration. However, growth was suppressed at higher concentrations exceeding 100 μg L<sup>−1</sup>. Addition of Cr(VI) in the cell culture medium showed a negative effect on quantum yield (<i>F<sub>v</sub>/F<sub>m</sub></i>), and a photosynthetic inhibition of >65% was noted in both species at 500 μg L<sup>−1</sup>. However, the lipid gravimetric analysis presented inner cell lipid content up to 36% and 30% of dry weight biomass for <i>M. pulchellum</i> and <i>M. pusillum,</i> respectively. The fatty acids profiles of both microalgae species showed higher levels of hexadecenoic acid as well as ω3, ω6, and ω7 fatty acids. The effect of Cr(VI) on photosynthesis and lipid accumulation in both microalgae species was concentration and exposure time dependent. This shows that an appropriate concentration of Cr(VI) in culture medium could be beneficial for higher lipid accumulation in freshwater eukaryotic microalgae species. |
| first_indexed | 2024-03-10T12:14:48Z |
| format | Article |
| id | doaj.art-ca13311dcbcc4eeb8730296962df3e46 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T12:14:48Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-ca13311dcbcc4eeb8730296962df3e462023-11-21T15:59:42ZengMDPI AGEnergies1996-10732021-04-01148226010.3390/en14082260The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte MicroalgaeKhawaja Muhammad Imran Bashir0Hyeon-Jun Lee1Sana Mansoor2Alexander Jahn3Man-Gi Cho4German Engineering Research and Development Center, LSTME-Busan, Busan 46742, KoreaDepartment of Chemical and Biological Engineering, Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg Busan Campus, Busan 46742, KoreaDepartment of Environmental Engineering, College of Engineering, Dong-A University, Busan 49315, KoreaGerman Engineering Research and Development Center, LSTME-Busan, Busan 46742, KoreaDepartment of Biotechnology, Division of Energy and Bioengineering, Dongseo University, Busan 47011, KoreaHeavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium—Cr(VI) on photosynthesis and lipid accumulation in <i>Mucidosphaerium pulchellum</i> and <i>Micractinium pusillum</i> exposed to different concentrations (0–500 μg L<sup>−1</sup>) was investigated for 11 days. A significant (<i>p</i> < 0.05) increase in lipid content was observed with increasing Cr(VI) concentration. However, growth was suppressed at higher concentrations exceeding 100 μg L<sup>−1</sup>. Addition of Cr(VI) in the cell culture medium showed a negative effect on quantum yield (<i>F<sub>v</sub>/F<sub>m</sub></i>), and a photosynthetic inhibition of >65% was noted in both species at 500 μg L<sup>−1</sup>. However, the lipid gravimetric analysis presented inner cell lipid content up to 36% and 30% of dry weight biomass for <i>M. pulchellum</i> and <i>M. pusillum,</i> respectively. The fatty acids profiles of both microalgae species showed higher levels of hexadecenoic acid as well as ω3, ω6, and ω7 fatty acids. The effect of Cr(VI) on photosynthesis and lipid accumulation in both microalgae species was concentration and exposure time dependent. This shows that an appropriate concentration of Cr(VI) in culture medium could be beneficial for higher lipid accumulation in freshwater eukaryotic microalgae species.https://www.mdpi.com/1996-1073/14/8/2260fatty acidsfreshwater microalgaegrowth kineticsheavy metal<i>Micractinium pusillum</i><i>Mucidosphaerium pulchellum</i> |
| spellingShingle | Khawaja Muhammad Imran Bashir Hyeon-Jun Lee Sana Mansoor Alexander Jahn Man-Gi Cho The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae Energies fatty acids freshwater microalgae growth kinetics heavy metal <i>Micractinium pusillum</i> <i>Mucidosphaerium pulchellum</i> |
| title | The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae |
| title_full | The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae |
| title_fullStr | The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae |
| title_full_unstemmed | The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae |
| title_short | The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae |
| title_sort | effect of chromium on photosynthesis and lipid accumulation in two chlorophyte microalgae |
| topic | fatty acids freshwater microalgae growth kinetics heavy metal <i>Micractinium pusillum</i> <i>Mucidosphaerium pulchellum</i> |
| url | https://www.mdpi.com/1996-1073/14/8/2260 |
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