Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA
Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institut...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224421000651 |
| _version_ | 1818648332352880640 |
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| author | Lauren Shusterman Antoinette E. Marsh Priscilla H. Joyner Greg Habing |
| author_facet | Lauren Shusterman Antoinette E. Marsh Priscilla H. Joyner Greg Habing |
| author_sort | Lauren Shusterman |
| collection | DOAJ |
| description | Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used a variety of nematode detection methods with various flotation solutions. Optimization of Trichuris egg detection is necessary for monitoring collections. Fecal and soil optimized protocols were generated in this study using samples containing Trichuris eggs from multiple semi free-ranging zoological institutions. First, Sheather's sugar (specific gravity (SG) 1.27), sucrose (SG 1.40), magnesium sulfate (SG 1.26), and zinc sulfate (SG 1.18) were compared as flotation solutions by quantitative eggs per gram using a modified Stoll method. Then a soil recovery method was optimized comparing Tween 20, sodium hydroxide, Dawn™ (Procter and Gamble) detergent, and sodium chloride as liberating solutions to free eggs from the soil. We found that Sheather's sugar and sucrose solutions were the most effective for Trichuris egg detection, and either sodium hydroxide or sodium chloride liberated eggs from soil. |
| first_indexed | 2024-12-17T01:16:45Z |
| format | Article |
| id | doaj.art-ca15fd5a022d490e88c7ecc0195947c2 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-12-17T01:16:45Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-ca15fd5a022d490e88c7ecc0195947c22022-12-21T22:08:58ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442021-08-0115208213Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USALauren Shusterman0Antoinette E. Marsh1Priscilla H. Joyner2Greg Habing3Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Rd, Columbus, OH, 43210, USADepartment of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Rd, Columbus, OH, 43210, USA; Corresponding author. Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Rd, Columbus, OH 43212, USA.The Wilds, 14000 International Road, Cumberland, OH, 43732, USADepartment of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Rd, Columbus, OH, 43210, USATrichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used a variety of nematode detection methods with various flotation solutions. Optimization of Trichuris egg detection is necessary for monitoring collections. Fecal and soil optimized protocols were generated in this study using samples containing Trichuris eggs from multiple semi free-ranging zoological institutions. First, Sheather's sugar (specific gravity (SG) 1.27), sucrose (SG 1.40), magnesium sulfate (SG 1.26), and zinc sulfate (SG 1.18) were compared as flotation solutions by quantitative eggs per gram using a modified Stoll method. Then a soil recovery method was optimized comparing Tween 20, sodium hydroxide, Dawn™ (Procter and Gamble) detergent, and sodium chloride as liberating solutions to free eggs from the soil. We found that Sheather's sugar and sucrose solutions were the most effective for Trichuris egg detection, and either sodium hydroxide or sodium chloride liberated eggs from soil.http://www.sciencedirect.com/science/article/pii/S2213224421000651TrichurisGiraffeNematodeFecesSoilModified stoll |
| spellingShingle | Lauren Shusterman Antoinette E. Marsh Priscilla H. Joyner Greg Habing Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA International Journal for Parasitology: Parasites and Wildlife Trichuris Giraffe Nematode Feces Soil Modified stoll |
| title | Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA |
| title_full | Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA |
| title_fullStr | Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA |
| title_full_unstemmed | Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA |
| title_short | Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA |
| title_sort | detection of trichuris eggs in feces and soil from giraffe giraffa camelopardalis and other hoofstock enclosures under human care in the usa |
| topic | Trichuris Giraffe Nematode Feces Soil Modified stoll |
| url | http://www.sciencedirect.com/science/article/pii/S2213224421000651 |
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