Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences
Six local Egyptian commercial cultivars of family Lamiaceae, two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme) were analyzed for ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences. All sa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2020-03-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
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| Online Access: | https://ajs.journals.ekb.eg/article_83159_354d243d8735ab9da902948c50e69475.pdf |
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| author | Hadir Mansour Rania Younis F Eldomiati M Rashed S Hassanein |
| author_facet | Hadir Mansour Rania Younis F Eldomiati M Rashed S Hassanein |
| author_sort | Hadir Mansour |
| collection | DOAJ |
| description | Six local Egyptian commercial cultivars of family Lamiaceae, two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme) were analyzed for ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences. All samples successfully amplified the ± 630 bp fragment. Additionally, the results of alignment analysis using BLASTN tools divulged that the sequence of DNA rbcL for the two local basil cultivars (Basil1 & Basil2) has similarities with (Ocimum basilicum, Ocimum tenuiflorum, Ocimum kilimandscharicum and Ocimum gratissimum) 100, 99.69, 99.37 and 99.06 respectively. In addition, two local mint cultivars (Mint 1 and Mint 2) has similarities with Mentha spicata, Mentha_pulegium, Mentha canadensis and Mentha menthaefolia, 99.85, 99.84, 99.69 and 99.53 respectively. For thyme local cultivars (Thyme1 and Thyme2), Thym1 cultivar sample genotype is genetic closely with species, (Thymus alsinoides and Thymus sibthorpii) with 99.69 and 99.84 respectively and they located nearest from the cluster (Thymus genus) members in phylogenetic trees while, Thym2 is located after the cluster with Artemisia genus belonging to family Asteraceae. The reason of this result may be occurring due to that a Thym2 genotype comes from local marketing, which some of them are selling it as a thymus genus however, it is belongs to Artemisia genus and has similarities with with seven species of Artemisia genus (Artemisia absinthium, Artemisia gmelinii, Artemisia selengensis, Artemisia scoparia, Artemisia maritima, Artemisia capillaris and Artemisia fukudo). Using of rbcL DNA barcode proves to be effective in identifying the plants from the family level up to the genus level. This study demonstrates the efficiency of using rbcL barcoding primer to classify family Lamiaceae phylogenetically. It is also concluded that the rbcL gene showed genuine potentials to distinguish the plant Egypt species under investigation into the proper family and genus. s to distinguish the plant Egypt species under investigation into the proper family and genus. |
| first_indexed | 2024-03-07T14:18:46Z |
| format | Article |
| id | doaj.art-ca189e00464245f3812ca425071d8076 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:54:37Z |
| publishDate | 2020-03-01 |
| publisher | The Union of Arab Universities |
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| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-ca189e00464245f3812ca425071d80762024-03-07T17:38:05ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852020-03-0128122923710.21608/ajs.2020.26513.118483159Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequencesHadir Mansour0Rania Younis1F Eldomiati2M Rashed3S Hassanein4Genetics Dept., Fac. Agric., Ain Shams UniversityGenetics Dept., Fac. Agric,, Ain Shams Univ.Genetics Dept., Fac. Agric., Ain Shams Univ.Dean of the Higher Institute for Agricultural Co-operationGenetics Dept., Fac. Agric., Ain ShamsSix local Egyptian commercial cultivars of family Lamiaceae, two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme) were analyzed for ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences. All samples successfully amplified the ± 630 bp fragment. Additionally, the results of alignment analysis using BLASTN tools divulged that the sequence of DNA rbcL for the two local basil cultivars (Basil1 & Basil2) has similarities with (Ocimum basilicum, Ocimum tenuiflorum, Ocimum kilimandscharicum and Ocimum gratissimum) 100, 99.69, 99.37 and 99.06 respectively. In addition, two local mint cultivars (Mint 1 and Mint 2) has similarities with Mentha spicata, Mentha_pulegium, Mentha canadensis and Mentha menthaefolia, 99.85, 99.84, 99.69 and 99.53 respectively. For thyme local cultivars (Thyme1 and Thyme2), Thym1 cultivar sample genotype is genetic closely with species, (Thymus alsinoides and Thymus sibthorpii) with 99.69 and 99.84 respectively and they located nearest from the cluster (Thymus genus) members in phylogenetic trees while, Thym2 is located after the cluster with Artemisia genus belonging to family Asteraceae. The reason of this result may be occurring due to that a Thym2 genotype comes from local marketing, which some of them are selling it as a thymus genus however, it is belongs to Artemisia genus and has similarities with with seven species of Artemisia genus (Artemisia absinthium, Artemisia gmelinii, Artemisia selengensis, Artemisia scoparia, Artemisia maritima, Artemisia capillaris and Artemisia fukudo). Using of rbcL DNA barcode proves to be effective in identifying the plants from the family level up to the genus level. This study demonstrates the efficiency of using rbcL barcoding primer to classify family Lamiaceae phylogenetically. It is also concluded that the rbcL gene showed genuine potentials to distinguish the plant Egypt species under investigation into the proper family and genus. s to distinguish the plant Egypt species under investigation into the proper family and genus.https://ajs.journals.ekb.eg/article_83159_354d243d8735ab9da902948c50e69475.pdfdna barcoderbclfamily lamiaceaeblastnphylogenetic |
| spellingShingle | Hadir Mansour Rania Younis F Eldomiati M Rashed S Hassanein Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences Arab Universities Journal of Agricultural Sciences dna barcode rbcl family lamiaceae blastn phylogenetic |
| title | Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences |
| title_full | Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences |
| title_fullStr | Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences |
| title_full_unstemmed | Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences |
| title_short | Phylogenetic analyses of some Egyptian genus of Lamiaceae family using rbcL sequences |
| title_sort | phylogenetic analyses of some egyptian genus of lamiaceae family using rbcl sequences |
| topic | dna barcode rbcl family lamiaceae blastn phylogenetic |
| url | https://ajs.journals.ekb.eg/article_83159_354d243d8735ab9da902948c50e69475.pdf |
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