Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media
The detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-12-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/16/12/2179 |
| _version_ | 1798005850533527552 |
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| author | Kyukwang Kim Duckyu Choi Hwijoon Lim Hyeongkeun Kim Jessie S. Jeon |
| author_facet | Kyukwang Kim Duckyu Choi Hwijoon Lim Hyeongkeun Kim Jessie S. Jeon |
| author_sort | Kyukwang Kim |
| collection | DOAJ |
| description | The detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern marker. The quantification of bacterial growth is possible as the bacterial growth in the culturing vessel blurs the marker image, which is placed on the back of the vessel, and the blurring results in a decrease in the high-frequency spectrum region of the marker image. The experiment results show that the FFT (fast Fourier transform)-based growth detection method is robust to the variations in the type of bacterial carrier and vessels ranging from the culture tubes to the microfluidic devices. Moreover, the automated incubator and image acquisition system are developed to be used as a comprehensive in situ detection system. We expect that this result can be applied in the automation of biological experiments, such as the Antibiotics Susceptibility Test or toxicity measurement. Furthermore, the simple framework of the proposed growth measurement method may be further utilized as an effective and convenient method for building point-of-care devices for developing countries. |
| first_indexed | 2024-04-11T12:45:32Z |
| format | Article |
| id | doaj.art-ca18ed3f7d5e46e490e3596db127e0a6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:45:32Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ca18ed3f7d5e46e490e3596db127e0a62022-12-22T04:23:21ZengMDPI AGSensors1424-82202016-12-011612217910.3390/s16122179s16122179Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture MediaKyukwang Kim0Duckyu Choi1Hwijoon Lim2Hyeongkeun Kim3Jessie S. Jeon4Urban Robotics Laboratory, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, KoreaDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, KoreaSchool of Electrical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, KoreaDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, KoreaDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, KoreaThe detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern marker. The quantification of bacterial growth is possible as the bacterial growth in the culturing vessel blurs the marker image, which is placed on the back of the vessel, and the blurring results in a decrease in the high-frequency spectrum region of the marker image. The experiment results show that the FFT (fast Fourier transform)-based growth detection method is robust to the variations in the type of bacterial carrier and vessels ranging from the culture tubes to the microfluidic devices. Moreover, the automated incubator and image acquisition system are developed to be used as a comprehensive in situ detection system. We expect that this result can be applied in the automation of biological experiments, such as the Antibiotics Susceptibility Test or toxicity measurement. Furthermore, the simple framework of the proposed growth measurement method may be further utilized as an effective and convenient method for building point-of-care devices for developing countries.http://www.mdpi.com/1424-8220/16/12/2179bacterial growthvision markerfast Fourier transformationmicrofluidics |
| spellingShingle | Kyukwang Kim Duckyu Choi Hwijoon Lim Hyeongkeun Kim Jessie S. Jeon Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media Sensors bacterial growth vision marker fast Fourier transformation microfluidics |
| title | Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media |
| title_full | Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media |
| title_fullStr | Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media |
| title_full_unstemmed | Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media |
| title_short | Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media |
| title_sort | vision marker based in situ examination of bacterial growth in liquid culture media |
| topic | bacterial growth vision marker fast Fourier transformation microfluidics |
| url | http://www.mdpi.com/1424-8220/16/12/2179 |
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