One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
Abstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decrea...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03014-2 |
| _version_ | 1818429662202691584 |
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| author | Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico |
| author_facet | Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico |
| author_sort | Maude Gerbaix |
| collection | DOAJ |
| description | Abstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decreased the trabecular bone volume (−64% vs. Habitat Control), dramatically increased the bone resorption (+140% vs. Habitat Control) and induced marrow adiposity invasion. At the diaphysis, cortical thinning associated with periosteal resorption was observed. In the Flight animal group, the osteocyte lacunae displayed a reduced volume and a more spherical shape (synchrotron radiation analyses), and empty lacunae were highly increased (+344% vs. Habitat Control). Tissue-level mechanical cortical properties (i.e., hardness and modulus) were locally decreased by spaceflight, whereas the mineral characteristics and collagen maturity were unaffected. In the vertebrae, spaceflight decreased the overall bone volume and altered the modulus in the periphery of the trabecular struts. Despite normalized osteoclastic activity and an increased osteoblast number, bone recovery was not observed 8 days after landing. In conclusion, spaceflight induces osteocyte death, which may trigger bone resorption and result in bone mass and microstructural deterioration. Moreover, osteocyte cell death, lacunae mineralization and fatty marrow, which are hallmarks of ageing, may impede tissue maintenance and repair. |
| first_indexed | 2024-12-14T15:21:05Z |
| format | Article |
| id | doaj.art-ca18ee232ea8420da5ef90bbd29606f1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:21:05Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ca18ee232ea8420da5ef90bbd29606f12022-12-21T22:56:10ZengNature PortfolioScientific Reports2045-23222017-06-017111210.1038/s41598-017-03014-2One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletonsMaude Gerbaix0Vasily Gnyubkin1Delphine Farlay2Cécile Olivier3Patrick Ammann4Guillaume Courbon5Norbert Laroche6Rachel Genthial7Hélène Follet8Françoise Peyrin9Boris Shenkman10Guillemette Gauquelin-Koch11Laurence Vico12French National Centre for Space StudiesINSERM, UMR 1059, University of Lyon, University Jean MonnetINSERM, UMR 1033, University of Lyon, University Claude Bernard Lyon 1University of Lyon, INSERM U1206, France and European Synchrotron Radiation Facility, CS40220Division of Bone Diseases, Department of Internal Medicine Specialties, Geneva University Hospitals and Faculty of MedicineFrench National Centre for Space StudiesINSERM, UMR 1059, University of Lyon, University Jean MonnetCNRS UMR 5588, University of Grenoble AlpesINSERM, UMR 1033, University of Lyon, University Claude Bernard Lyon 1University of Lyon, INSERM U1206, France and European Synchrotron Radiation Facility, CS40220Institute for Biomedical Problems, Russian Academy of SciencesFrench National Centre for Space StudiesINSERM, UMR 1059, University of Lyon, University Jean MonnetAbstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decreased the trabecular bone volume (−64% vs. Habitat Control), dramatically increased the bone resorption (+140% vs. Habitat Control) and induced marrow adiposity invasion. At the diaphysis, cortical thinning associated with periosteal resorption was observed. In the Flight animal group, the osteocyte lacunae displayed a reduced volume and a more spherical shape (synchrotron radiation analyses), and empty lacunae were highly increased (+344% vs. Habitat Control). Tissue-level mechanical cortical properties (i.e., hardness and modulus) were locally decreased by spaceflight, whereas the mineral characteristics and collagen maturity were unaffected. In the vertebrae, spaceflight decreased the overall bone volume and altered the modulus in the periphery of the trabecular struts. Despite normalized osteoclastic activity and an increased osteoblast number, bone recovery was not observed 8 days after landing. In conclusion, spaceflight induces osteocyte death, which may trigger bone resorption and result in bone mass and microstructural deterioration. Moreover, osteocyte cell death, lacunae mineralization and fatty marrow, which are hallmarks of ageing, may impede tissue maintenance and repair.https://doi.org/10.1038/s41598-017-03014-2 |
| spellingShingle | Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons Scientific Reports |
| title | One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons |
| title_full | One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons |
| title_fullStr | One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons |
| title_full_unstemmed | One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons |
| title_short | One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons |
| title_sort | one month spaceflight compromises the bone microstructure tissue level mechanical properties osteocyte survival and lacunae volume in mature mice skeletons |
| url | https://doi.org/10.1038/s41598-017-03014-2 |
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