Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study
<i>Background and Objectives</i>: Wound healing is a dynamic process that can be compromised in patients with chronic and metabolic conditions or unhealthy lifestyles. Numerous medical substances designed for topical use, charged with compounds that promote the healing process, have been...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/57/3/285 |
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| author | Elena Canciani Riccardo Sirello Gaia Pellegrini Dolaji Henin Mariachiara Perrotta Marilisa Toma Nataliya Khomchyna Claudia Dellavia |
| author_facet | Elena Canciani Riccardo Sirello Gaia Pellegrini Dolaji Henin Mariachiara Perrotta Marilisa Toma Nataliya Khomchyna Claudia Dellavia |
| author_sort | Elena Canciani |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Wound healing is a dynamic process that can be compromised in patients with chronic and metabolic conditions or unhealthy lifestyles. Numerous medical substances designed for topical use, charged with compounds that promote the healing process, have been developed to improve wound healing, especially in compromised subjects. The present study aimed to extend our understanding of the in vivo effects of a hyaluronic acid gel charged with amino acids (HAplus gel, Aminogam gel<sup>®</sup> Errekappa Euroterapici spa, Milan, Italy) and study the in vitro effects of the same gel charged with additional substances in an attempt to optimize its formulation. <i>Materials and Methods:</i> In a randomized controlled split-mouth clinical and histological trial, HAplus gel was tested on the gingival tissue of the lower third molar post-extraction socket. The gingiva was collected at the time of extraction (T0) and ten days after the extraction (T1) to be histologically analyzed. During the second stage of the study, culture media with HAplus gel and vitamin C and E at different concentrations (TEST) were tested on human gingival fibroblasts and compared to the HAplus-enriched medium (HA-Control). <i>Results:</i> Histological and immunohistochemical analysis of collected gingiva showed higher microvascular density and collagen fibers organized in closely packed and well-oriented bundles in sites treated with HAplus gel. In the in vitro study, all TEST groups showed an increased viability from 24 h to 48 h. After 24 h, the viability percentage in all experimental groups was below 100% of the HA-Control, demonstrating a mild toxicity. After 48 h from seeding, the TEST groups’ viability grew significantly compared to HA-Control. <i>Conclusions:</i> These encouraging preliminary results suggest that the use of HAplus gel enriched with vitamins C and E may be beneficial in patients with conditions that impair soft tissue healing. |
| first_indexed | 2024-03-10T13:07:10Z |
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| id | doaj.art-ca19df523a2648708d9d6d922e1984e9 |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T13:07:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-ca19df523a2648708d9d6d922e1984e92023-11-21T11:03:56ZengMDPI AGMedicina1010-660X1648-91442021-03-0157328510.3390/medicina57030285Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro StudyElena Canciani0Riccardo Sirello1Gaia Pellegrini2Dolaji Henin3Mariachiara Perrotta4Marilisa Toma5Nataliya Khomchyna6Claudia Dellavia7Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via Mangiagalli 31, 20133 Milan, Italy<i>Background and Objectives</i>: Wound healing is a dynamic process that can be compromised in patients with chronic and metabolic conditions or unhealthy lifestyles. Numerous medical substances designed for topical use, charged with compounds that promote the healing process, have been developed to improve wound healing, especially in compromised subjects. The present study aimed to extend our understanding of the in vivo effects of a hyaluronic acid gel charged with amino acids (HAplus gel, Aminogam gel<sup>®</sup> Errekappa Euroterapici spa, Milan, Italy) and study the in vitro effects of the same gel charged with additional substances in an attempt to optimize its formulation. <i>Materials and Methods:</i> In a randomized controlled split-mouth clinical and histological trial, HAplus gel was tested on the gingival tissue of the lower third molar post-extraction socket. The gingiva was collected at the time of extraction (T0) and ten days after the extraction (T1) to be histologically analyzed. During the second stage of the study, culture media with HAplus gel and vitamin C and E at different concentrations (TEST) were tested on human gingival fibroblasts and compared to the HAplus-enriched medium (HA-Control). <i>Results:</i> Histological and immunohistochemical analysis of collected gingiva showed higher microvascular density and collagen fibers organized in closely packed and well-oriented bundles in sites treated with HAplus gel. In the in vitro study, all TEST groups showed an increased viability from 24 h to 48 h. After 24 h, the viability percentage in all experimental groups was below 100% of the HA-Control, demonstrating a mild toxicity. After 48 h from seeding, the TEST groups’ viability grew significantly compared to HA-Control. <i>Conclusions:</i> These encouraging preliminary results suggest that the use of HAplus gel enriched with vitamins C and E may be beneficial in patients with conditions that impair soft tissue healing.https://www.mdpi.com/1648-9144/57/3/285hyaluronic acidvitaminsgelimpaired wound healingoral mucosadental personalized medicine |
| spellingShingle | Elena Canciani Riccardo Sirello Gaia Pellegrini Dolaji Henin Mariachiara Perrotta Marilisa Toma Nataliya Khomchyna Claudia Dellavia Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study Medicina hyaluronic acid vitamins gel impaired wound healing oral mucosa dental personalized medicine |
| title | Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study |
| title_full | Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study |
| title_fullStr | Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study |
| title_full_unstemmed | Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study |
| title_short | Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study |
| title_sort | effects of vitamin and amino acid enriched hyaluronic acid gel on the healing of oral mucosa in vivo and in vitro study |
| topic | hyaluronic acid vitamins gel impaired wound healing oral mucosa dental personalized medicine |
| url | https://www.mdpi.com/1648-9144/57/3/285 |
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