Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice
Summary: Neural activity is heterogeneous across different cortical areas and can change during learning. Here, we describe a protocol for longitudinal in vivo two-photon calcium imaging with an ultra-large cranial window that exposes most of the dorsal cortex in head-fixed mice. The large cranial w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002234 |
| _version_ | 1817984452073095168 |
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| author | Ryoma Hattori Takaki Komiyama |
| author_facet | Ryoma Hattori Takaki Komiyama |
| author_sort | Ryoma Hattori |
| collection | DOAJ |
| description | Summary: Neural activity is heterogeneous across different cortical areas and can change during learning. Here, we describe a protocol for longitudinal in vivo two-photon calcium imaging with an ultra-large cranial window that exposes most of the dorsal cortex in head-fixed mice. The large cranial window allows optical access to any dorsal cortical areas in individual mice. This protocol enables longitudinal tracking of neural activity from various cortical areas at cellular resolution to understand the cortical computations during behavioral tasks.For complete details on the use and execution of this protocol, please refer to Hattori et al. (2019), and Hattori and Komiyama, 2022a. |
| first_indexed | 2024-04-13T23:45:40Z |
| format | Article |
| id | doaj.art-ca19f2eab20d4875841c4ad0c1020ee3 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T23:45:40Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ca19f2eab20d4875841c4ad0c1020ee32022-12-22T02:24:21ZengElsevierSTAR Protocols2666-16672022-06-0132101343Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed miceRyoma Hattori0Takaki Komiyama1Neurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California, San Diego, La Jolla, CA 92093, USA; Corresponding authorNeurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California, San Diego, La Jolla, CA 92093, USA; Corresponding authorSummary: Neural activity is heterogeneous across different cortical areas and can change during learning. Here, we describe a protocol for longitudinal in vivo two-photon calcium imaging with an ultra-large cranial window that exposes most of the dorsal cortex in head-fixed mice. The large cranial window allows optical access to any dorsal cortical areas in individual mice. This protocol enables longitudinal tracking of neural activity from various cortical areas at cellular resolution to understand the cortical computations during behavioral tasks.For complete details on the use and execution of this protocol, please refer to Hattori et al. (2019), and Hattori and Komiyama, 2022a.http://www.sciencedirect.com/science/article/pii/S2666166722002234MicroscopyModel OrganismsNeuroscience |
| spellingShingle | Ryoma Hattori Takaki Komiyama Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice STAR Protocols Microscopy Model Organisms Neuroscience |
| title | Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice |
| title_full | Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice |
| title_fullStr | Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice |
| title_full_unstemmed | Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice |
| title_short | Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice |
| title_sort | longitudinal two photon calcium imaging with ultra large cranial window for head fixed mice |
| topic | Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002234 |
| work_keys_str_mv | AT ryomahattori longitudinaltwophotoncalciumimagingwithultralargecranialwindowforheadfixedmice AT takakikomiyama longitudinaltwophotoncalciumimagingwithultralargecranialwindowforheadfixedmice |