Efficiency of Semen Cryopreservation in Stallions
Differences in the cryotolerance of spermatozoa exist among stallions, but it remains to be determined to what extent such differences are affected by breed. In this study, post-thaw semen quality in stallions presented for semen cryopreservation was analysed retrospectively (1012 ejaculates from 13...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/6/1033 |
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| author | Jörg Aurich Juliane Kuhl Alexander Tichy Christine Aurich |
| author_facet | Jörg Aurich Juliane Kuhl Alexander Tichy Christine Aurich |
| author_sort | Jörg Aurich |
| collection | DOAJ |
| description | Differences in the cryotolerance of spermatozoa exist among stallions, but it remains to be determined to what extent such differences are affected by breed. In this study, post-thaw semen quality in stallions presented for semen cryopreservation was analysed retrospectively (1012 ejaculates from 134 stallions of 5 breeds). The percentage of frozen–thawed ejaculates acceptable for artificial insemination (AI) and the number of insemination doses per cryopreserved ejaculate was calculated. Logistic regression analysis revealed sperm motility in raw semen as the most important explanatory variable for the percentage of cryopreserved ejaculates with a post-thaw quality acceptable for AI. Of the other variables included into the model, stallion age was the most important parameter with more acceptable ejaculates in younger than in older stallions. Logistic regression also showed more acceptable frozen–thawed ejaculates in Arab stallions versus Warmbloods, Quarter Horses and Icelandic horses. The analysis thus demonstrates differences in the percentage of acceptable cryopreserved ejaculates among horse breeds. Season was a less relevant explanatory variable for percentage of acceptable cryopreserved ejaculates. Logistic regression revealed total sperm count as the most important variable determining the number of cryopreserved semen doses obtained per acceptable ejaculate. In conclusion, logistic regression analysis revealed stallion age and breed as explanatory variables for the percentage of cryopreserved ejaculates acceptable for AI. |
| first_indexed | 2024-03-10T19:12:27Z |
| format | Article |
| id | doaj.art-ca1b15f076eb478486fea96a6a38ff55 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:12:27Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ca1b15f076eb478486fea96a6a38ff552023-11-20T03:43:59ZengMDPI AGAnimals2076-26152020-06-01106103310.3390/ani10061033Efficiency of Semen Cryopreservation in StallionsJörg Aurich0Juliane Kuhl1Alexander Tichy2Christine Aurich3Obstetrics, Gynaecology and Andrology, Department for Small Animals and Horses, Vetmeduni Vienna, 1210 Vienna, AustriaArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, 1210 Vienna, AustriaBioinformatics and Biostatistics, Department for Biomedical Science, Vetmeduni Vienna, 1210 Vienna, AustriaArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, 1210 Vienna, AustriaDifferences in the cryotolerance of spermatozoa exist among stallions, but it remains to be determined to what extent such differences are affected by breed. In this study, post-thaw semen quality in stallions presented for semen cryopreservation was analysed retrospectively (1012 ejaculates from 134 stallions of 5 breeds). The percentage of frozen–thawed ejaculates acceptable for artificial insemination (AI) and the number of insemination doses per cryopreserved ejaculate was calculated. Logistic regression analysis revealed sperm motility in raw semen as the most important explanatory variable for the percentage of cryopreserved ejaculates with a post-thaw quality acceptable for AI. Of the other variables included into the model, stallion age was the most important parameter with more acceptable ejaculates in younger than in older stallions. Logistic regression also showed more acceptable frozen–thawed ejaculates in Arab stallions versus Warmbloods, Quarter Horses and Icelandic horses. The analysis thus demonstrates differences in the percentage of acceptable cryopreserved ejaculates among horse breeds. Season was a less relevant explanatory variable for percentage of acceptable cryopreserved ejaculates. Logistic regression revealed total sperm count as the most important variable determining the number of cryopreserved semen doses obtained per acceptable ejaculate. In conclusion, logistic regression analysis revealed stallion age and breed as explanatory variables for the percentage of cryopreserved ejaculates acceptable for AI.https://www.mdpi.com/2076-2615/10/6/1033breedcryopreservationhorsesemen |
| spellingShingle | Jörg Aurich Juliane Kuhl Alexander Tichy Christine Aurich Efficiency of Semen Cryopreservation in Stallions Animals breed cryopreservation horse semen |
| title | Efficiency of Semen Cryopreservation in Stallions |
| title_full | Efficiency of Semen Cryopreservation in Stallions |
| title_fullStr | Efficiency of Semen Cryopreservation in Stallions |
| title_full_unstemmed | Efficiency of Semen Cryopreservation in Stallions |
| title_short | Efficiency of Semen Cryopreservation in Stallions |
| title_sort | efficiency of semen cryopreservation in stallions |
| topic | breed cryopreservation horse semen |
| url | https://www.mdpi.com/2076-2615/10/6/1033 |
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