Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community
Previously, we found that in glucose-limited Saccharomyces cerevisiae colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic or glycolytic states. In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organizat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/57609 |
| _version_ | 1811201430174826496 |
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| author | Sriram Varahan Vaibhhav Sinha Adhish Walvekar Sandeep Krishna Sunil Laxman |
| author_facet | Sriram Varahan Vaibhhav Sinha Adhish Walvekar Sandeep Krishna Sunil Laxman |
| author_sort | Sriram Varahan |
| collection | DOAJ |
| description | Previously, we found that in glucose-limited Saccharomyces cerevisiae colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic or glycolytic states. In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al., 2019). We now discover that the plasticity of use of a non-limiting resource, aspartate, controls both resource production and the emergence of heterogeneous cell states, based on differential metabolic budgeting. In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. This metabolic plasticity of aspartate enables carbon-nitrogen budgeting, thereby driving the biochemical self-organization of distinct cell states. Through this organization, cells in each state exhibit true division of labor, providing growth/survival advantages for the whole community. |
| first_indexed | 2024-04-12T02:21:34Z |
| format | Article |
| id | doaj.art-ca1b8dbbedb04f29a6a2f576d5dec48e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:21:34Z |
| publishDate | 2020-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ca1b8dbbedb04f29a6a2f576d5dec48e2022-12-22T03:52:07ZengeLife Sciences Publications LtdeLife2050-084X2020-09-01910.7554/eLife.57609Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal communitySriram Varahan0https://orcid.org/0000-0002-3609-4032Vaibhhav Sinha1https://orcid.org/0000-0002-5169-5485Adhish Walvekar2https://orcid.org/0000-0001-7344-7653Sandeep Krishna3https://orcid.org/0000-0002-0581-173XSunil Laxman4https://orcid.org/0000-0002-0861-5080InStem - Institute for Stem Cell Science and Regenerative Medicine, Bangalore, IndiaSimons Centre for the Study of Living Machines, National Center for Biological Sciences, Tata Institute for Fundamental Research, Bangalore, India; Manipal Academy of Higher Education, Manipal, IndiaInStem - Institute for Stem Cell Science and Regenerative Medicine, Bangalore, IndiaSimons Centre for the Study of Living Machines, National Center for Biological Sciences, Tata Institute for Fundamental Research, Bangalore, IndiaInStem - Institute for Stem Cell Science and Regenerative Medicine, Bangalore, IndiaPreviously, we found that in glucose-limited Saccharomyces cerevisiae colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic or glycolytic states. In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al., 2019). We now discover that the plasticity of use of a non-limiting resource, aspartate, controls both resource production and the emergence of heterogeneous cell states, based on differential metabolic budgeting. In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. This metabolic plasticity of aspartate enables carbon-nitrogen budgeting, thereby driving the biochemical self-organization of distinct cell states. Through this organization, cells in each state exhibit true division of labor, providing growth/survival advantages for the whole community.https://elifesciences.org/articles/57609division of laborself-organizationaspartatecross-feedingglycolysistrehalose |
| spellingShingle | Sriram Varahan Vaibhhav Sinha Adhish Walvekar Sandeep Krishna Sunil Laxman Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community eLife division of labor self-organization aspartate cross-feeding glycolysis trehalose |
| title | Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community |
| title_full | Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community |
| title_fullStr | Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community |
| title_full_unstemmed | Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community |
| title_short | Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community |
| title_sort | resource plasticity driven carbon nitrogen budgeting enables specialization and division of labor in a clonal community |
| topic | division of labor self-organization aspartate cross-feeding glycolysis trehalose |
| url | https://elifesciences.org/articles/57609 |
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