A method of direct measurement for the enzymatic determination of cholesteryl esters
A direct measurement method for the enzymatic determination of cholesteryl esters (CEs) without measuring total cholesterol (TC) and free cholesterol (FC) is described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE wa...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2004-02-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752031912X |
| _version_ | 1818715716016144384 |
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| author | Toshimi Mizoguchi Toshiyuki Edano Tomoyuki Koshi |
| author_facet | Toshimi Mizoguchi Toshiyuki Edano Tomoyuki Koshi |
| author_sort | Toshimi Mizoguchi |
| collection | DOAJ |
| description | A direct measurement method for the enzymatic determination of cholesteryl esters (CEs) without measuring total cholesterol (TC) and free cholesterol (FC) is described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or a fluorometric method. The measurement sensitivity of the fluorometric method was more than 20 times that of the colorimetric method. Optimal conditions of the assay were determined, and examples of measured CE in human plasma, rat liver, and cultured cells are indicated.The method of directly measuring CE was simple and has exceptional reproducibility compared with the technique of subtracting FC from TC using each measured TC and FC. |
| first_indexed | 2024-12-17T19:07:47Z |
| format | Article |
| id | doaj.art-ca1d4808c9304c73b6bf04dcacd862e0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T19:07:47Z |
| publishDate | 2004-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ca1d4808c9304c73b6bf04dcacd862e02022-12-21T21:35:57ZengElsevierJournal of Lipid Research0022-22752004-02-01452396401A method of direct measurement for the enzymatic determination of cholesteryl estersToshimi Mizoguchi0Toshiyuki Edano1Tomoyuki Koshi2Tokyo New Drug Research Laboratories II, Pharmaceutical Division, Kowa Co. Ltd., Tokyo 189-0022, JapanTokyo New Drug Research Laboratories II, Pharmaceutical Division, Kowa Co. Ltd., Tokyo 189-0022, JapanTokyo New Drug Research Laboratories II, Pharmaceutical Division, Kowa Co. Ltd., Tokyo 189-0022, JapanA direct measurement method for the enzymatic determination of cholesteryl esters (CEs) without measuring total cholesterol (TC) and free cholesterol (FC) is described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or a fluorometric method. The measurement sensitivity of the fluorometric method was more than 20 times that of the colorimetric method. Optimal conditions of the assay were determined, and examples of measured CE in human plasma, rat liver, and cultured cells are indicated.The method of directly measuring CE was simple and has exceptional reproducibility compared with the technique of subtracting FC from TC using each measured TC and FC.http://www.sciencedirect.com/science/article/pii/S002222752031912Xcholesterol assaycholesterol oxidase catalaseplasmalivertissue culture |
| spellingShingle | Toshimi Mizoguchi Toshiyuki Edano Tomoyuki Koshi A method of direct measurement for the enzymatic determination of cholesteryl esters Journal of Lipid Research cholesterol assay cholesterol oxidase catalase plasma liver tissue culture |
| title | A method of direct measurement for the enzymatic determination of cholesteryl esters |
| title_full | A method of direct measurement for the enzymatic determination of cholesteryl esters |
| title_fullStr | A method of direct measurement for the enzymatic determination of cholesteryl esters |
| title_full_unstemmed | A method of direct measurement for the enzymatic determination of cholesteryl esters |
| title_short | A method of direct measurement for the enzymatic determination of cholesteryl esters |
| title_sort | method of direct measurement for the enzymatic determination of cholesteryl esters |
| topic | cholesterol assay cholesterol oxidase catalase plasma liver tissue culture |
| url | http://www.sciencedirect.com/science/article/pii/S002222752031912X |
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