Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota
Phorate is a systemic, broad-spectrum organophosphorus insecticide. Although it is commonly used worldwide, phorate, like other pesticides, not only causes environmental pollution but also poses serious threats to human and animal health. Herein, we measured the blood glucose concentrations of high-...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1584 |
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| author | Tingting Cao Yajie Guo Dan Wang Zhiyang Liu Suli Huang Changfeng Peng Shaolin Wang Yang Wang Qi Lu Fan Xiao Zhaoyi Liang Sijia Zheng Jianzhong Shen Yongning Wu Ziquan Lv Yuebin Ke |
| author_facet | Tingting Cao Yajie Guo Dan Wang Zhiyang Liu Suli Huang Changfeng Peng Shaolin Wang Yang Wang Qi Lu Fan Xiao Zhaoyi Liang Sijia Zheng Jianzhong Shen Yongning Wu Ziquan Lv Yuebin Ke |
| author_sort | Tingting Cao |
| collection | DOAJ |
| description | Phorate is a systemic, broad-spectrum organophosphorus insecticide. Although it is commonly used worldwide, phorate, like other pesticides, not only causes environmental pollution but also poses serious threats to human and animal health. Herein, we measured the blood glucose concentrations of high-fat-diet-fed mice exposed to various concentrations of phorate (0, 0.005, 0.05, or 0.5 mg/kg); we also assessed the blood glucose concentrations of high-fat-diet-fed mice exposed to phorate; we also assessed the distribution characteristics of the resistance genes in the intestinal microbiota of these mice. We found that 0.005 and 0.5 mg/kg of phorate induced obvious hyperglycaemia in the high-fat-diet-fed mice. Exposure to phorate markedly reduced the abundance of <i>Akkermansia muciniphila</i> in the mouse intestine. The resistance genes <i>vanRG, tetW/N/W, acrD</i>, and <i>evgS</i> were significantly upregulated in the test group compared with the control group. Efflux pumping was the primary mechanism of drug resistance in the <i>Firmicutes, Proteobacteria, Bacteroidetes, Verrucomicrobia, Synergistetes, Spirochaetes</i>, and <i>Actinobacteria</i> found in the mouse intestine. Our findings indicate that changes in the abundance of the intestinal microbiota are closely related to the presence of antibiotic-resistant bacteria in the intestinal tract and the metabolic health of the host. |
| first_indexed | 2024-03-09T19:19:25Z |
| format | Article |
| id | doaj.art-ca1edd26f8e74d3f89700f5107fafeef |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T19:19:25Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ca1edd26f8e74d3f89700f5107fafeef2023-11-24T03:28:43ZengMDPI AGAntibiotics2079-63822022-11-011111158410.3390/antibiotics11111584Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal MicrobiotaTingting Cao0Yajie Guo1Dan Wang2Zhiyang Liu3Suli Huang4Changfeng Peng5Shaolin Wang6Yang Wang7Qi Lu8Fan Xiao9Zhaoyi Liang10Sijia Zheng11Jianzhong Shen12Yongning Wu13Ziquan Lv14Yuebin Ke15Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaThe Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, ChinaSchool of Public Health, Southern Medical University, Guangzhou 510515, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100091, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100091, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100091, ChinaFood Safety Research Unit (2019RU014), Chinese Academy of Medical Science, NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaPhorate is a systemic, broad-spectrum organophosphorus insecticide. Although it is commonly used worldwide, phorate, like other pesticides, not only causes environmental pollution but also poses serious threats to human and animal health. Herein, we measured the blood glucose concentrations of high-fat-diet-fed mice exposed to various concentrations of phorate (0, 0.005, 0.05, or 0.5 mg/kg); we also assessed the blood glucose concentrations of high-fat-diet-fed mice exposed to phorate; we also assessed the distribution characteristics of the resistance genes in the intestinal microbiota of these mice. We found that 0.005 and 0.5 mg/kg of phorate induced obvious hyperglycaemia in the high-fat-diet-fed mice. Exposure to phorate markedly reduced the abundance of <i>Akkermansia muciniphila</i> in the mouse intestine. The resistance genes <i>vanRG, tetW/N/W, acrD</i>, and <i>evgS</i> were significantly upregulated in the test group compared with the control group. Efflux pumping was the primary mechanism of drug resistance in the <i>Firmicutes, Proteobacteria, Bacteroidetes, Verrucomicrobia, Synergistetes, Spirochaetes</i>, and <i>Actinobacteria</i> found in the mouse intestine. Our findings indicate that changes in the abundance of the intestinal microbiota are closely related to the presence of antibiotic-resistant bacteria in the intestinal tract and the metabolic health of the host.https://www.mdpi.com/2079-6382/11/11/1584phoratehyperglycaemiaintestinal microbiotaresistance genes |
| spellingShingle | Tingting Cao Yajie Guo Dan Wang Zhiyang Liu Suli Huang Changfeng Peng Shaolin Wang Yang Wang Qi Lu Fan Xiao Zhaoyi Liang Sijia Zheng Jianzhong Shen Yongning Wu Ziquan Lv Yuebin Ke Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota Antibiotics phorate hyperglycaemia intestinal microbiota resistance genes |
| title | Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota |
| title_full | Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota |
| title_fullStr | Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota |
| title_full_unstemmed | Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota |
| title_short | Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota |
| title_sort | effect of phorate on the development of hyperglycaemia in mouse and resistance genes in intestinal microbiota |
| topic | phorate hyperglycaemia intestinal microbiota resistance genes |
| url | https://www.mdpi.com/2079-6382/11/11/1584 |
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