Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis
Interleukin-6 (IL-6) plays a crucial role in autoimmunity and chronic inflammation. This study aims to develop a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical point-of-care device for the rapid detection of IL-6 in patients with psoriasis. Precisely, a sandwich-based...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1251360/full |
| _version_ | 1797629518194671616 |
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| author | Rocco Cancelliere Terenzio Cosio Terenzio Cosio Elena Campione Martina Corvino Maria Pia D’Amico Laura Micheli Emanuela Signori Giorgio Contini Giorgio Contini |
| author_facet | Rocco Cancelliere Terenzio Cosio Terenzio Cosio Elena Campione Martina Corvino Maria Pia D’Amico Laura Micheli Emanuela Signori Giorgio Contini Giorgio Contini |
| author_sort | Rocco Cancelliere |
| collection | DOAJ |
| description | Interleukin-6 (IL-6) plays a crucial role in autoimmunity and chronic inflammation. This study aims to develop a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical point-of-care device for the rapid detection of IL-6 in patients with psoriasis. Precisely, a sandwich-based format immunosensor was developed using two primary antibodies (mAb-IL6 clone-5 and clone-7) and screen-printed electrodes modified with an inexpensive recycling electrochemical enhancing material, called biochar. mAb-IL6 clone-5 was used as a covalently immobilized capture bioreceptor on modified electrodes, and mAb-IL6 clone-7 was used to recognize the immunocomplex (Anti-IL6 clone-5 and IL-6) and form the sandwich. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to conduct electrochemical characterization of the layer-by-layer assembly of the immunosensor, while square wave voltammetry (SWV) was used to perform the sensing. The developed immunosensor demonstrated robust analytical performance in buffer solution, with a wide linear range (LR) by varying from 2 to 250 pg/mL, a good limit of detection (LOD) of 0.78 pg/mL and reproducibility (RSD<7%). In addition, a spectrophotometric ELISA kit was employed to validate the results obtained with the label-free device by analyzing twenty-five serum samples from control and patients affected by psoriasis. A strong correlation in terms of pg/mL concentration of IL-6 was found comparing the two methods, with the advantage for our label-free biosensor of an ease use and a quicker detection time. Based on IL-6 levels, the proposed immunosensor is a dependable, non-invasive screening device capable of predicting disease onset, progression, and treatment efficacy. |
| first_indexed | 2024-03-11T10:54:35Z |
| format | Article |
| id | doaj.art-ca2344e606ac4b75b812692916faeef5 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-03-11T10:54:35Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-ca2344e606ac4b75b812692916faeef52023-11-13T10:59:29ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-11-011110.3389/fchem.2023.12513601251360Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasisRocco Cancelliere0Terenzio Cosio1Terenzio Cosio2Elena Campione3Martina Corvino4Maria Pia D’Amico5Laura Micheli6Emanuela Signori7Giorgio Contini8Giorgio Contini9Department of Chemical Science and Technologies, University of Rome Tor Vergata, Roma, ItalyDermatology Unit, Department of Systems Medicine, University of Rome Tor Vergata, Roma, ItalyDepartment of Experimental Medicine, University of Rome Tor Vergata, Roma, ItalyDermatology Unit, Department of Systems Medicine, University of Rome Tor Vergata, Roma, ItalyDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, Roma, ItalyDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, Roma, ItalyDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, Roma, ItalyIstituto di Farmacologia Traslazionale-CNR (IFT-CNR), Roma, ItalyIstituto di Struttura Della Materia-CNR (ISM-CNR), Roma, ItalyDepartment of Physics, University of Rome Tor Vergata, Roma, ItalyInterleukin-6 (IL-6) plays a crucial role in autoimmunity and chronic inflammation. This study aims to develop a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical point-of-care device for the rapid detection of IL-6 in patients with psoriasis. Precisely, a sandwich-based format immunosensor was developed using two primary antibodies (mAb-IL6 clone-5 and clone-7) and screen-printed electrodes modified with an inexpensive recycling electrochemical enhancing material, called biochar. mAb-IL6 clone-5 was used as a covalently immobilized capture bioreceptor on modified electrodes, and mAb-IL6 clone-7 was used to recognize the immunocomplex (Anti-IL6 clone-5 and IL-6) and form the sandwich. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to conduct electrochemical characterization of the layer-by-layer assembly of the immunosensor, while square wave voltammetry (SWV) was used to perform the sensing. The developed immunosensor demonstrated robust analytical performance in buffer solution, with a wide linear range (LR) by varying from 2 to 250 pg/mL, a good limit of detection (LOD) of 0.78 pg/mL and reproducibility (RSD<7%). In addition, a spectrophotometric ELISA kit was employed to validate the results obtained with the label-free device by analyzing twenty-five serum samples from control and patients affected by psoriasis. A strong correlation in terms of pg/mL concentration of IL-6 was found comparing the two methods, with the advantage for our label-free biosensor of an ease use and a quicker detection time. Based on IL-6 levels, the proposed immunosensor is a dependable, non-invasive screening device capable of predicting disease onset, progression, and treatment efficacy.https://www.frontiersin.org/articles/10.3389/fchem.2023.1251360/fullinterleukin-6sandwich-based immunosensorbiocharserum samplespsoriasispoint-ofcare |
| spellingShingle | Rocco Cancelliere Terenzio Cosio Terenzio Cosio Elena Campione Martina Corvino Maria Pia D’Amico Laura Micheli Emanuela Signori Giorgio Contini Giorgio Contini Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis Frontiers in Chemistry interleukin-6 sandwich-based immunosensor biochar serum samples psoriasis point-ofcare |
| title | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
| title_full | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
| title_fullStr | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
| title_full_unstemmed | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
| title_short | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
| title_sort | label free electrochemical immunosensor as a reliable point of care device for the detection of interleukin 6 in serum samples from patients with psoriasis |
| topic | interleukin-6 sandwich-based immunosensor biochar serum samples psoriasis point-ofcare |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1251360/full |
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