High particle variability across siliconized and oil-free syringes and needles from the same lots
Abstract Previous studies have reported silicone oil (SO) applied to needles and syringes in the vitreous of patients after intravitreal injections. We evaluated four syringes (SR 1-mL insulin, Saldanha-Rodrigues; BD 1-mL Tuberculin Slip Tip, Becton–Dickinson; BD Ultra-Fine 0.3 mL, HSW Norm-Ject Tub...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84158-0 |
| _version_ | 1818998128646291456 |
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| author | Lydianne Lumack do Monte Agra Natasha Ferreira Santos da Cruz Vaida Linkuviene John F. Carpenter Michel Eid Farah Gustavo Barreto Melo Maurício Maia |
| author_facet | Lydianne Lumack do Monte Agra Natasha Ferreira Santos da Cruz Vaida Linkuviene John F. Carpenter Michel Eid Farah Gustavo Barreto Melo Maurício Maia |
| author_sort | Lydianne Lumack do Monte Agra |
| collection | DOAJ |
| description | Abstract Previous studies have reported silicone oil (SO) applied to needles and syringes in the vitreous of patients after intravitreal injections. We evaluated four syringes (SR 1-mL insulin, Saldanha-Rodrigues; BD 1-mL Tuberculin Slip Tip, Becton–Dickinson; BD Ultra-Fine 0.3 mL, HSW Norm-Ject Tuberculin, Henke Sass Wolf) and 10 needles (BD PrecisionGlide 27- and 30-gauge (G); BD Eclipse and JBP Nanoneedle 27-, 30-, 33- and 34-G; TSK Invisible Needle and 27 and 30-G Steriject Control Hub). The protein-free buffer samples injected into the syringes and needles under study were collected in an Eppendorf tube and taken to Flow imaging microscopy, that characterized the concentration and morphology of the microsized particles. The number of particles was analyzed. The coefficients of variation (CV) were the primary outcome. The Feltz and Miller test compared the CVs. The significance level was 5%. Numerous particles and high CVs were associated with both devices, needles and syringes; the comparisons among them did not reach significance. The BD Ultrafine 0.3 mL syringe (149.7%) had the highest CV and the SO-free HSW Norm-Ject (66.4%) syringe the lowest, and the TSK Invisible needle (149.5%) had the highest and the BD Precision Glide 30G needle (35.9%) needle the lowest. In conclusion, particle release, including those with SO morphology, varied greatly among instruments, even from the same lots, which is relevant considering that fewer particles are injected into some eyes compared with others. |
| first_indexed | 2024-12-20T21:56:36Z |
| format | Article |
| id | doaj.art-ca280611157a482a9e6e6b7b5bccca36 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:56:36Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ca280611157a482a9e6e6b7b5bccca362022-12-21T19:25:27ZengNature PortfolioScientific Reports2045-23222021-02-011111610.1038/s41598-021-84158-0High particle variability across siliconized and oil-free syringes and needles from the same lotsLydianne Lumack do Monte Agra0Natasha Ferreira Santos da Cruz1Vaida Linkuviene2John F. Carpenter3Michel Eid Farah4Gustavo Barreto Melo5Maurício Maia6Hospital de Olhos de SergipeDepartment of Ophthalmology, Federal University of São PauloDepartment of Pharmaceutical Sciences, University of ColoradoDepartment of Pharmaceutical Sciences, University of ColoradoDepartment of Ophthalmology, Federal University of São PauloHospital de Olhos de SergipeDepartment of Ophthalmology, Federal University of São PauloAbstract Previous studies have reported silicone oil (SO) applied to needles and syringes in the vitreous of patients after intravitreal injections. We evaluated four syringes (SR 1-mL insulin, Saldanha-Rodrigues; BD 1-mL Tuberculin Slip Tip, Becton–Dickinson; BD Ultra-Fine 0.3 mL, HSW Norm-Ject Tuberculin, Henke Sass Wolf) and 10 needles (BD PrecisionGlide 27- and 30-gauge (G); BD Eclipse and JBP Nanoneedle 27-, 30-, 33- and 34-G; TSK Invisible Needle and 27 and 30-G Steriject Control Hub). The protein-free buffer samples injected into the syringes and needles under study were collected in an Eppendorf tube and taken to Flow imaging microscopy, that characterized the concentration and morphology of the microsized particles. The number of particles was analyzed. The coefficients of variation (CV) were the primary outcome. The Feltz and Miller test compared the CVs. The significance level was 5%. Numerous particles and high CVs were associated with both devices, needles and syringes; the comparisons among them did not reach significance. The BD Ultrafine 0.3 mL syringe (149.7%) had the highest CV and the SO-free HSW Norm-Ject (66.4%) syringe the lowest, and the TSK Invisible needle (149.5%) had the highest and the BD Precision Glide 30G needle (35.9%) needle the lowest. In conclusion, particle release, including those with SO morphology, varied greatly among instruments, even from the same lots, which is relevant considering that fewer particles are injected into some eyes compared with others.https://doi.org/10.1038/s41598-021-84158-0 |
| spellingShingle | Lydianne Lumack do Monte Agra Natasha Ferreira Santos da Cruz Vaida Linkuviene John F. Carpenter Michel Eid Farah Gustavo Barreto Melo Maurício Maia High particle variability across siliconized and oil-free syringes and needles from the same lots Scientific Reports |
| title | High particle variability across siliconized and oil-free syringes and needles from the same lots |
| title_full | High particle variability across siliconized and oil-free syringes and needles from the same lots |
| title_fullStr | High particle variability across siliconized and oil-free syringes and needles from the same lots |
| title_full_unstemmed | High particle variability across siliconized and oil-free syringes and needles from the same lots |
| title_short | High particle variability across siliconized and oil-free syringes and needles from the same lots |
| title_sort | high particle variability across siliconized and oil free syringes and needles from the same lots |
| url | https://doi.org/10.1038/s41598-021-84158-0 |
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