A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses
The detection of megalocytiviruses, especially the infectious spleen and kidney necrosis virus (ISKNV), in ornamental fish has increased with the rapid growth of the ornamental fish industry. In this study, dwarf gourami fin (DGF) cells derived from the caudal fin of the dwarf gourami (<i>Tric...
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/6/829 |
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| author | Ye-Jin Jeong Kwang-Il Kim |
| author_facet | Ye-Jin Jeong Kwang-Il Kim |
| author_sort | Ye-Jin Jeong |
| collection | DOAJ |
| description | The detection of megalocytiviruses, especially the infectious spleen and kidney necrosis virus (ISKNV), in ornamental fish has increased with the rapid growth of the ornamental fish industry. In this study, dwarf gourami fin (DGF) cells derived from the caudal fin of the dwarf gourami (<i>Trichogaster lalius</i>), which is highly susceptible to red sea bream iridovirus (RSIV) and ISKNV, were established and characterized. The DGF cells were grown at temperatures ranging from 25 °C to 30 °C in Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum and were subcultured for more than 100 passages, predominantly with epithelial-like cells. DGF cells had a diploid chromosome number of 2<i>n</i> = 44. Although the initial purpose of this study was to establish a cell line for the causative agents of red sea bream iridoviral disease (RSIV and ISKNV), DGF cells were also susceptible to rhabdoviruses (viral hemorrhagic septicemia virus, hirame rhabdovirus, and spring viraemia of carp virus), exhibiting a significant cytopathic effect characterized by cell rounding and lysis. Additionally, viral replication and virion morphology were confirmed using virus-specific conventional polymerase chain reaction and transmission electron microscopy. Furthermore, both RSIV and ISKNV were replicated at high concentrations in DGF cells compared to other cell lines. Notably, the DGF cells maintained a monolayer during ISKNV infection, indicating the possibility of persistent infection. Thus, DGF can be used for viral diagnosis and may play a critical role in advancing our understanding of ISKNV pathogenesis. |
| first_indexed | 2024-03-11T02:45:06Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T02:45:06Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-ca2bbf7c1a0c42d0a3aff5744c552a8b2023-11-18T09:23:08ZengMDPI AGBiology2079-77372023-06-0112682910.3390/biology12060829A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish VirusesYe-Jin Jeong0Kwang-Il Kim1Department of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of KoreaThe detection of megalocytiviruses, especially the infectious spleen and kidney necrosis virus (ISKNV), in ornamental fish has increased with the rapid growth of the ornamental fish industry. In this study, dwarf gourami fin (DGF) cells derived from the caudal fin of the dwarf gourami (<i>Trichogaster lalius</i>), which is highly susceptible to red sea bream iridovirus (RSIV) and ISKNV, were established and characterized. The DGF cells were grown at temperatures ranging from 25 °C to 30 °C in Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum and were subcultured for more than 100 passages, predominantly with epithelial-like cells. DGF cells had a diploid chromosome number of 2<i>n</i> = 44. Although the initial purpose of this study was to establish a cell line for the causative agents of red sea bream iridoviral disease (RSIV and ISKNV), DGF cells were also susceptible to rhabdoviruses (viral hemorrhagic septicemia virus, hirame rhabdovirus, and spring viraemia of carp virus), exhibiting a significant cytopathic effect characterized by cell rounding and lysis. Additionally, viral replication and virion morphology were confirmed using virus-specific conventional polymerase chain reaction and transmission electron microscopy. Furthermore, both RSIV and ISKNV were replicated at high concentrations in DGF cells compared to other cell lines. Notably, the DGF cells maintained a monolayer during ISKNV infection, indicating the possibility of persistent infection. Thus, DGF can be used for viral diagnosis and may play a critical role in advancing our understanding of ISKNV pathogenesis.https://www.mdpi.com/2079-7737/12/6/829dwarf gouramiprimary cellinfectious spleen and kidney necrosis virusred sea bream iridovirus |
| spellingShingle | Ye-Jin Jeong Kwang-Il Kim A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses Biology dwarf gourami primary cell infectious spleen and kidney necrosis virus red sea bream iridovirus |
| title | A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses |
| title_full | A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses |
| title_fullStr | A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses |
| title_full_unstemmed | A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses |
| title_short | A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (<i>Trichogaster lalius</i>) and Its Susceptibility to Fish Viruses |
| title_sort | new cell line derived from the caudal fin of the dwarf gourami i trichogaster lalius i and its susceptibility to fish viruses |
| topic | dwarf gourami primary cell infectious spleen and kidney necrosis virus red sea bream iridovirus |
| url | https://www.mdpi.com/2079-7737/12/6/829 |
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